6,340 research outputs found

    Precocious Locomotor Behavior Begins in the Egg: Development of Leg Muscle Patterns for Stepping in the Chick

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    BACKGROUND:The chicken is capable of adaptive locomotor behavior within hours after hatching, yet little is known of the processes leading to this precocious skill. During the final week of incubation, chick embryos produce distinct repetitive limb movements that until recently had not been investigated. In this study we examined the leg muscle patterns at 3 time points as development of these spontaneous movements unfolds to determine if they exhibit attributes of locomotion reported in hatchlings. We also sought to determine whether the deeply flexed posture and movement constraint imposed by the shell wall modulate the muscle patterns. METHODOLOGY/PRINCIPAL FINDINGS:Synchronized electromyograms for leg muscles, force and video were recorded continuously from embryos while in their naturally flexed posture at embryonic day (E) 15, E18 and E20. We tested for effects of leg posture and constraint by removing shell wall anterior to the foot. Results indicated that by E18, burst onset time distinguished leg muscle synergists from antagonists across a 10-fold range in burst frequencies (1-10 Hz), and knee extensors from ankle extensors in patterns comparable to locomotion at hatching. However, burst durations did not scale with step cycle duration in any of the muscles recorded. Despite substantially larger leg movements after shell removal, the knee extensor was the only muscle to vary its activity, and extensor muscles often failed to participate. To further clarify if the repetitive movements are likely locomotor-related, we examined bilateral coordination of ankle muscles during repetitive movements at E20. In all cases ankle muscles exhibited a bias for left/right alternation. CONCLUSIONS/SIGNIFICANCE:Collectively, the findings lead us to conclude that the repetitive leg movements in late stage embryos are locomotor-related and a fundamental link in the establishment of precocious locomotor skill. The potential importance of differences between embryonic and posthatching locomotion is discussed

    High Yielding Acid-Catalysed Hydrolysis of Cellulosic Polysaccharides and Native Biomass into Low Molecular Weight Sugars in Mixed Ionic Liquid Systems

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    © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. Ionic media comprising 1-butyl-3-methylimidazolium chloride and the acidic deep eutectic solvent choline chloride/oxalic acid as co-solvent-catalyst, very efficiently convert various cellulosic substrates, including native cellulosic biomass, into water-soluble carbohydrates. The optimum reaction systems yield a narrow range of low molecular weight carbohydrates directly from cellulose, lignocellulose, or algal saccharides, in high yields and selectivities up to 98 %. Cellulose possesses significant potential as a renewable platform from which to generate large volumes of green replacements to many petrochemical products. Within this goal, the production of low molecular weight saccharides from cellulosic substances is the key to success. Native cellulose and lignocellulosic feedstocks are less accessible for such transformations and depolymerisation of polysaccharides remains a primary challenge to be overcome. In this study, we identify the catalytic activity associated with selected deep eutectic solvents that favours the hydrolysis of polysaccharides and develop reaction conditions to improve the outcomes of desirable low molecular weight sugars. We successfully apply the chemistry to raw bulk, non-pretreated cellulosic substances

    Mature open cows are rarely persistently infected with bovine viral diarrhea virus

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    Bovine viral diarrhea virus (BVDv) is an immunosuppressive virus affecting cattle in a multitude of ways. The varied presentation makes this disease difficult to identify in cow herds and the signs of a BVD infection may be very subtle. The syndrome causes economic problems by reducing herd fertility and increasing disease rates. The persistently infected (PI) animal is a unique reservoir for BVDv. These cattle are the result of in utero exposure to the noncytopathic biotype of BVDv prior to the development of a competent fetal immune system at about 125 days of gestation. Persistently infected animals are the primary method for the disease to propagate over time. PI cattle consistently shed BVD virus in relatively high levels and this exposure to the breeding herd can result in new PI calves. PI animals propagate BVDv in the herd and decrease pregnancy percentages compared to herds without PI animals. Farms must assess risk and manage for biosecurity when purchasing adult animals with an unknown history of disease exposure. Breeding herds that introduce new animals to the herd face the risk of importing a BVD PI animal. To mitigate this risk, PI animals must be accurately identified prior to herd introduction, but visual appraisal is not an accurate method of discovering these animals. Multiple diagnostic tests are available to determine the BVD status of incoming animals and all have an associated cost. Economic feasibility of determining the BVD PI status of animals depends to a large degree on the frequency with which PI animals occur in a population. Previous research has illustrated that PI calves entering the feedyard phase of production are fairly rare (about three per 1,000 calves); however, very little work has been done in mature animals. This project provides an estimate of BVD PI frequency for a specific population. This assessment should allow the formulation of a BVD-specific risk management plan which addresses the economic efficiency of testing mature females upon arrival. The primary objective of this research is to determine the prevalence of BVD PI animals in a population of young (3- to 6-year-old) cows purchased as non-pregnant mature animals. The results can guide biosecurity decisions for producers when purchasing and introducing this class of animal to the herd

    Continuous loading of a magnetic trap

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    We have realized a scheme for continuous loading of a magnetic trap (MT). ^{52}Cr atoms are continuously captured and cooled in a magneto-optical trap (MOT). Optical pumping to a metastable state decouples atoms from the cooling light. Due to their high magnetic moment (6 Bohr magnetons), low-field seeking metastable atoms are trapped in the magnetic quadrupole field provided by the MOT. Limited by inelastic collisions between atoms in the MOT and in the MT, we load 10^8 metastable atoms at a rate of 10^8 atoms/s below 100 microkelvin into the MT. After loading we can perform optical repumping to realize a MT of ground state chromium atoms.Comment: 4 pages, 4 figures, version 2, modified references, included additional detailed information, minor changes in figure 3 and in tex
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