Interpretation of Solar Magnetic Field Strength Observations

This study based on longitudinal Zeeman effect magnetograms and spectral line scans investigates the dependence of solar surface magnetic fields on the spectral line used and the way the line is sampled in order to estimate the magnetic flux emerging above the solar atmosphere and penetrating to the corona from magnetograms of the Mt. Wilson 150-foot tower synoptic program (MWO). We have compared the synoptic program \lambda5250\AA line of Fe I to the line of Fe I at \lambda5233\AA since this latter line has a broad shape with a profile that is nearly linear over a large portion of its wings. The present study uses five pairs of sampling points on the $\lambda5233$\AA line. We recommend adoption of the field determined with a line bisector method with a sampling point as close as possible to the line core as the best estimate of the emergent photospheric flux. The combination of the line profile measurements and the cross-correlation of fields measured simultaneously with \lambda5250\AA and \lambda5233\AA yields a formula for the scale factor 1/\delta that multiplies the MWO synoptic magnetic fields. The new calibration shows that magnetic fields measured by the MDI system on the SOHO spacecraft are equal to 0.619+/-0.018 times the true value at a center-to-limb position 30 deg. Berger and Lites (2003) found this factor to be 0.64+/-0.013 based on a comparison the the Advanced Stokes Polarimeter.Comment: Accepted by Solar Physic

Photospheric Velocities Measured at Mt. Wilson Show Zonal and Sectoral Flows Compose the Torsional Oscillations

The methods for reducing the observations from the 150-foot Tower Telescope on Mt.~Wilson are reviewed and a new method for determining the North/South (sectoral) and the East/West (zonal) velocity components is described and applied. Due to a calibration problem with the data prior to 1983, only observations between 1983 and 2013 are presented at this time. After subtraction of latitude dependent averages over the 30-year period of observation the residual deviations in the sectoral and zonal flow velocities are well synchronized and correspond to what is widely recognized as the Torsional Oscillations. Both flow components need to be included in any model that replicates the Torsional Oscillations.Comment: 15 pages, 5 figures, 1 table. Submitted to Solar Physics 7/12/202

Automated whole-cell patch-clamp electrophysiology of neurons in vivo

Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.National Institutes of Health (U.S.) (NIH EUREKA Award program (1R01NS075421))National Institutes of Health (U.S.) ((NIH) Director′s New Innovator Award (DP2OD002002)National Science Foundation (U.S.) ((NSF) CAREER award (CBET 1053233))New York Stem Cell Foundation (Robertson Neuroscience Award)Dr. Gerald Burnett and Marjorie BurnettNational Science Foundation (U.S.) (grant CISE 1110947)National Science Foundation (U.S.) (grant EHR 0965945)American Heart Association (10GRNT4430029

Diagnosis and Management of Complex Reentrant Arrhythmias Involving the His-Purkinje System

The His-Purkinje system is a network of bundles and fibres comprised of specialised cells that allow for coordinated, synchronous activation of the ventricles. Although the histology and physiology of the His-Purkinje system have been studied for more than a century, its role in ventricular arrhythmias has recently been discovered with the ongoing elucidation of the mechanisms leading to both benign and life-threatening arrhythmias. Studies of Purkinje-cell electrophysiology show multiple mechanisms responsible for ventricular arrhythmias, including enhanced automaticity, triggered activity and reentry. The variation in functional properties of Purkinje cells in different areas of the His-Purkinje system underlie the propensity for reentry within Purkinje fibres in structurally normal and abnormal hearts. Catheter ablation is an effective therapy in nearly all forms of reentrant arrhythmias involving Purkinje tissue. However, identifying those at risk of developing fascicular arrhythmias is not yet possible. Future research is needed to understand the precise molecular and functional changes resulting in these arrhythmias

Low-Speed Wind Tunnel Tests of Two Waverider Configuration Models

A definitive measurement of the low-speed flight characteristics of waverider-based aircraft is required to augment the overall design database for this important class of vehicles which have great potential for efficient high-speed flight. Two separate waverider-derived vehicles were tested; one in the 14- by 22-Foot Tunnel and the other in the 12-Foot Low Speed Tunnel at Langley Research Center. These tests provided measurements of moments and forces about all three axes, control effectiveness, flow field characteristics and the effects of configuration changes. The results of these tunnel tests are summarized and the subsonic aerodynamic characteristics of the two configurations are shown

Violence and vulnerability:Children’s strategies and the logic of violence in Burundi

This study explores how children in conflict-affected Burundi deal with violence in their everyday lives. Focusing on schools as a context in which children are prepared for further roles in society, child-centred, qualitative and mixed-methods research was conducted at 36 primary schools throughout Burundi. Findings reveal that children use a variety of strategies to deal with violence they encounter. These strategies reflect a learned 'logic of violence', matching dynamics of violence and vulnerability in society at large. Children's strategies are ultimately aimed at reducing vulnerability to (future) violence and indicative of the omnipresence of violence and uncertainty in Burundi. (C) 2019 The Authors. Children & Society published by National Children's Bureau and John Wiley & Sons LtdPublic Health and primary car

Search for Short-Term Periodicities in the Sun's Surface Rotation: A Revisit

The power spectral analyses of the Sun's surface equatorial rotation rate determined from the Mt. Wilson daily Doppler velocity measurements during the period 3 December 1985 to 5 March 2007 suggests the existence of 7.6 year, 2.8 year, 1.47 year, 245 day, 182 day and 158 day periodicities in the surface equatorial rotation rate during the period before 1996. However, there is no variation of any kind in the more accurately measured data during the period after 1995. That is, the aforementioned periodicities in the data during the period before the year 1996 may be artifacts of the uncertainties of those data due to the frequent changes in the instrumentation of the Mt. Wilson spectrograph. On the other hand, the temporal behavior of most of the activity phenomena during cycles 22 (1986-1996) and 23 (after 1997) is considerably different. Therefore, the presence of the aforementioned short-term periodicities during the last cycle and absence of them in the current cycle may, in principle, be real temporal behavior of the solar rotation during these cycles.Comment: 11 pages, 6 figures, accepted for publication in Solar Physic

Rabies screen reveals GPe control of cocaine-triggered plasticity.

Identification of neural circuit changes that contribute to behavioural plasticity has routinely been conducted on candidate circuits that were preselected on the basis of previous results. Here we present an unbiased method for identifying experience-triggered circuit-level changes in neuronal ensembles in mice. Using rabies virus monosynaptic tracing, we mapped cocaine-induced global changes in inputs onto neurons in the ventral tegmental area. Cocaine increased rabies-labelled inputs from the globus pallidus externus (GPe), a basal ganglia nucleus not previously known to participate in behavioural plasticity triggered by drugs of abuse. We demonstrated that cocaine increased GPe neuron activity, which accounted for the increase in GPe labelling. Inhibition of GPe activity revealed that it contributes to two forms of cocaine-triggered behavioural plasticity, at least in part by disinhibiting dopamine neurons in the ventral tegmental area. These results suggest that rabies-based unbiased screening of changes in input populations can identify previously unappreciated circuit elements that critically support behavioural adaptations

Ethical and methodological issues in engaging young people living in poverty with participatory research methods

This paper discusses the methodological and ethical issues arising from a project that focused on conducting a qualitative study using participatory techniques with children and young people living in disadvantage. The main aim of the study was to explore the impact of poverty on children and young people's access to public and private services. The paper is based on the author's perspective of the first stage of the fieldwork from the project. It discusses the ethical implications of involving children and young people in the research process, in particular issues relating to access and recruitment, the role of young people's advisory groups, use of visual data and collection of data in young people's homes. The paper also identifies some strategies for addressing the difficulties encountered in relation to each of these aspects and it considers the benefits of adopting participatory methods when conducting research with children and young people

Network inference analysis identifies an APRR2-like gene linked to pigment accumulation in tomato and pepper fruits

Carotenoids represent some of the most important secondary metabolites in the human diet, and tomato (Solanum lycopersicum) is a rich source of these health-promoting compounds. In this work, a novel and fruit-related regulator of pigment accumulation in tomato has been identified by artificial neural network inference analysis and its function validated in transgenic plants. A tomato fruit gene regulatory network was generated using artificial neural network inference analysis and transcription factor gene expression profiles derived from fruits sampled at various points during development and ripening. One of the transcription factor gene expression profiles with a sequence related to an Arabidopsis (Arabidopsis thaliana) ARABIDOPSIS PSEUDO RESPONSE REGULATOR2-LIKE gene (APRR2-Like) was up-regulated at the breaker stage in wild-type tomato fruits and, when overexpressed in transgenic lines, increased plastid number, area, and pigment content, enhancing the levels of chlorophyll in immature unripe fruits and carotenoids in red ripe fruits. Analysis of the transcriptome of transgenic lines overexpressing the tomato APPR2-Like gene revealed up-regulation of several ripening-related genes in the overexpression lines, providing a link between the expression of this tomato gene and the ripening process. A putative ortholog of the tomato APPR2-Like gene in sweet pepper (Capsicum annuum) was associated with pigment accumulation in fruit tissues. We conclude that the function of this gene is conserved across taxa and that it encodes a protein that has an important role in ripening