Reaction of Bcl-2+ cells of the parietal lobe cortex of rats with experimental diabetes mellitus to ischemia-reperfusion

The peculiarities of anti-apoptotic mechanisms in the nerve and glial cells of the parietal lobe cortex are studied by the density changes of Bcl-2+-cells location and concentration of Bcl-2 protein in them in rats with diabetes mellitus in the dynamics of ischemic-reperfusion cerebral injury. Bilateral carotid ischemia-reperfusion is found to inhibit anti-apoptotic reaction of nerve cells of the parietal lobe cortex in the early and late post-ischemic periods at the expense of decreased number of Bcl-2+-cells and concentration of Bcl-2 protein in them. In glial cells during the early period of ischemic-reperfusion injury the activity of anti-apoptotic processes intensifies to some extent by means of increased concentration of Bcl-2 protein in them, and on the 12th day the activity of anti-apoptotic processes decrease at the expense of reduced amount of Bcl-2+-cells and the content of Bcl-2 protein in them. Three-month streptozotocin-induced diabetes increases the location density of positive by protein Bcl-2 nerve cells in the parietal lobe cortex without influencing on the location density of Bcl-2+-glial cells and concentration of Bcl-2 protein in them. 20-minute ischemia with one-hour reperfusion in animals with diabetes mellitus considerably enhances anti-apoptotic potential of glial cells in the parietal lobe cerebral cortex at the expense of increased amount of Bcl-2+-cells and the concentration of Bcl-2 protein in them without reliable effect on these indices in nerve cells. On the 12th day of ischemic-reperfusion period in rats with diabetes mellitus, the intensity of anti-apoptotic processes in general decreases both in nerve and glial cells of the parietal lobe cortex

Changes of diurnal organization of free-radical modification of macromolecules, antioxidant defense in healthy people exposed to prolonged photoperiod

Prolonged artificial light period as a consequence of civilisation, as proven, may disturb some physiological circadian patterns. This may be a background for illnesses development. The aim of investigation was to compare daily profiles of free radical oxidation products and antioxidant patterns in people with usual and disturbed sleeping regimen. 41 healthy volunteers were examined. 6 blood samples were taken during 24 h. 24 people had stable sleep regimen from 11.00 p.m. to 7.00 a.m. (group 1); rest went to bed systematically around 2.00-3.00 a.m. at least 3-4 days per week (group 2). Free radical oxidation products (malonyc dialdehyde, products of free radical protein modification) and antioxidants (restored glutathione content, activity of catalase, gluthathio-neperoxidase, glutathione transferase) were measured by standard biochemical methods. All results were approved statistically. Maximal values of free radical oxidation metabolytes were inverse to antioxidant activity profiles in both groups. But, we established that daily profiles of all investigated parameters were violated in people with late falling asleep. They had lower daily average levels of antioxidants and higher - of products of macromolecules' free radical modification. In addition, shift of acrophase of free radical oxidation

SYNTROPY OF UNCLASSIFIED COMPLEXES OF MULTIPLE CONGENITAL MALFORMATIONS

Statistical analysis of unclassified complexes multiple of congenital malformations was performed . Frequency of the most frequent combination of defects consisting of multiple congenital malformations was determined , syntropy index was defined. "Watching congenital malformations" (anus atresia, cleft lip/palate, congenital umbilical hernia, clubfoot) according to the analysis of association and syntropy index can be used as a marker as to congenital heart defects, central nervous system and kidney

Morphological, morphometric and laser polarimetry characteristics of epithelial tissue of kidneys that have been structurally reorganized

The article deals with the results of study of structural organization of epithelial tissue of the kidneys that were exposed to the combined influence of stress factor and metal salts. It has been determined, that of all the methods, used in the research, morphological changes are characterized by the method of laser polarimetry in full measure

Evolution of Mueller Matrix Images of the Myometrium for the Optical Anisotropy Oncological Changes

The optical model of polycrystalline networks of myometium is suggested. The results of investigating the interrelation between the values correlation (correlation area, asymmetry coefficient and autocorrelation function excess) and fractal (dispersion of logarithmic dependencies of power spectra) parameters are presented. They characterize the distributions of Mueller matrix elements in the points of laser images of myometrium histological sections. The criteria of differentiation of death coming reasons are determined

POLARIZATION PROPERTIES OF TISSUES OF SOME ENDOCRINE GLANDS

The polarization properties of the tissue of the endocrine glands based on histological section were studied in the paper. An investigation by means of the method of laser polarimetry of the thyroid, suprarenal and prostate tissue demonstrated the polarizationproperties of the glands of intact rats in health

Polarization Selection of Birefringence Polycrystalline Networks of Blood Plasma Layers

The principles of optical model of human bile polycrystalline structure are described. The results of investigating the interrelation between the values of statistical, c orrelation and fractal parameters are presented. They characterize the coordinate distributions of mutual polarization degree of the points of laser images of bile smears of cholelithiasis patients in combination with other pathologies. The diagnostic criteria of the cholelithiasis nascency and its severity degree differentiation are determined

Енергії заміщення аніонів та катіонів у кадмій та цинк телуридах

Energy substitution of cations and anions in ZnTe i CdTe by atoms from first and seventh group of the periodic table were calculated using the method of bonding orbitals. Based on the results conclusions about the most likely types of defects by doping.Методом зв’язуючих орбіталей розраховані енергії заміщення катіонів та аніонів у ZnTe i CdTe атомами першої та сьомої груп Періодичної таблиці. На основі отриманих результатів зроблено висновки про найімовірніші типи дефектів при легуванні

Leading medical and scientific center of the Chernivtsi region (1944-1954)

В представленном вниманию читателя исследовании предложен анализ процесса становления и развития Черновицкого медицинского института в период 1944-1954 годов. Работа основана на целом корпусе нового архивного материала с глубоким анализом всех процессов жизнедеятельности учебного заведения. Статья оснащена таблицами с цифровыми данными, фотографиями, часть из которых впервые печатается, биографической информацией про отдельных сотрудниках института. Доказательная база проведенного исследования дает полное право утверждать, что Черновицкий медицинский институт в период 1944-1954 гг. стал ведущим лечебным и научным центром медицины в Черновицкой области. The study presented to the reader’s attention suggests an analysis of the foundation and development of the Chernivtsi Medical Institute in the period of 1944-1954. The work is based on archival and museum materials from the funds of the State Archives of the Chernivtsi Region (SACR), the Scientific Archives of the Bukovinian State Medical University, the History and Medical Museum of the University, a number of works on the history of Bukovina (including the History of Medicine) and local studies of Ukrainian, Austrian, Romanian researchers of the second half of the 19th and 20th centuries, publications in the local press about the state of medicine and the achievement of the CDMI. Most of these materials are published for the first time. The process of establishment of the institution, complex economic, social, cultural, sanitary-hygienic, epidemiological postwar conditions of the period of the 1940s was subjected to thorough analysis. The main premises of the Institute, representing historical and architectural value, are described. The activity of workers of the CDMI during the investigation of the causes and elimination of the consequences of the foci of goiter endemy, skin and venereal diseases, the fight against tuberculosis, infectious diseases (epidemic and recurrent typhus, typhoid, paratyphoid), malaria, dysentery, diphtheria, scarlet fever, meningitis, measles, influenza. The technique of expedition works is clarified, scientific work is described for revealing the causes of diseases and their effective treatment, the most important publications of scientific research results. Among the inherent diseases of this territory, Bukovina hemorrhagic fever was found, methods of its treatment were developed. There presented an interesting analysis of new tools and methods of treatment of patients proposed by the Institute's staff. Among them, the method of research with radioisotopes (Labeled Atoms) at the Department of Biochemistry, therapeutic physical culture in the clinics of the CDMI, novocain blockade in the clinic of ear, nose, throat, tissue therapy in the obstetric-gynecological clinic. The researchers paid attention to the consultations and surgery made in the districts of the region and medical help given to the population of neighboring regions, the process of improving the skills of medical workers of the whole region. Quite interesting is the description of research by Professor BP. Aleksandrovsky in the history of medicine. The work is equipped with tables containing statistic data in various spheres of the institution activity, photographs that are first published in scientific work, objective biographical information about individual scientists. In the conclusions the authors insist that the evidence based on the presented research entitles to assert that the staff of the Chernivtsi State Medical Institute in the period 1944-1954 managed to turn the institution into a leading medical and scientific center of medicine in the Chernivtsi region