The potential of postharvest silicon dips to regulate phenolics in citrus peel as a method to mitigate chilling injury in lemons

This study investigated the ability of silicon dips to enhance the phenolic content in order to reduce the incidence of chilling injury in lemon fruit. Fruits were obtained from two farms and dipped in 0, 50, 150 and 250 mg L-1 solutions of K2SiO3 for 30 min and afterward, fruit were air dried and waxed. Thereafter, fruits were stored at -0.5°C and sampled after 28 days for evaluation of phenolic content and chilling injury symptoms. Chilling susceptible fruit sourced from Ithala farm had significantly lower phenolics and flavonoids concentration when compared with chilling resistant lemons from Ukulinga farm. Phenolic and flavonoids content was improved by dipping fruit in silicon for almost all the concentrations. Moreover, 50 mg L-1 reduced the occurrence of chilling injury symptoms whilst high silicon concentrations increased chilling injury. In conclusion, silicon dips have an ability to reduce chilling injury symptoms in lemons; however, low concentrations should be used.Keywords: Silicon, lemon, antioxidants, phenolics, chilling injuryAfrican Journal of Biotechnology, Vol. 12(13), pp. 1482-148

GPAQ-R: development and psychometric properties of a version of the general practice assessment questionnaire for use for revalidation by general practitioners in the UK.

BACKGROUND: The General Practice Assessment Questionnaire (GPAQ) has been widely used to assess patient experience in general practice in the UK since 2004. In 2013, new regulations were introduced by the General Medical Council (GMC) requiring UK doctors to undertake periodic revalidation, which includes assessment of patient experience for individual doctors. We describe the development of a new version of GPAQ - GPAQ-R which addresses the GMC's requirements for revalidation as well as additional NHS requirements for surveys that GPs may need to carry out in their own practices. METHODS: Questionnaires were given out by doctors or practice staff after routine consultations in line with the guidance given by the General Medical Council for surveys to be used for revalidation. Data analysis and practice reports were provided independently. RESULTS: Data were analysed for questionnaires from 7258 patients relating to 164 GPs in 29 general practices. Levels of missing data were generally low (typically 4.5-6%). The number of returned questionnaires required to achieve reliability of 0.7 were around 35 for individual doctor communication items and 29 for a composite score based on doctor communication items. This suggests that the responses to GPAQ-R had similar reliability to the GMC's own questionnaire and we recommend 30 completed GPAQ-R questionnaires are sufficient for revalidation purposes. However, where an initial screen raises concern, the survey might be repeated with 50 completed questionnaires in order to increase reliability. CONCLUSIONS: GPAQ-R is a development of a well-established patient experience questionnaire used in general practice in the UK since 2004. This new version can be recommended for use in order to meet the UK General Medical Council's requirements for surveys to be used in revalidation of doctors. It also meets the needs of GPs to ask about patient experience relating to aspects of practice care that are not specific to individual general practitioners (e.g. receptionists, telephone access) which meet other survey requirements of the National Health Service in England. Use of GPAQ-R has the potential to reduce the number of surveys that GPs need to carry out in their practices to meet the various regulatory requirements which they face

A Very Hot, High Redshift Cluster of Galaxies: More Trouble for Omega_0 = 1

We have observed the most distant (z=0.829) cluster of galaxies in the Einstein Extended Medium Sensitivity Survey, with the ASCA and ROSAT satellites. We find an X-ray temperature of 12.3 +3.1/-2.2 keV for this cluster, and the ROSAT map reveals significant substructure. The high temperature of MS1054-0321 is consistent with both its approximate velocity dispersion, based on the redshifts of 12 cluster members we have obtained at the Keck and the Canada-France-Hawaii telescopes, and with its weak lensing signature. The X-ray temperature of this cluster implies a virial mass ~ 7.4 x 10^14 h^-1 solar masses, if the mean matter density in the universe equals the critical value, or larger if Omega_0 < 1. Finding such a hot, massive cluster in the EMSS is extremely improbable if clusters grew from Gaussian perturbations in an Omega_0 = 1 universe. Combining the assumptions that Omega_0 = 1 and that the intial perturbations were Gaussian with the observed X-ray temperature function at low redshift, we show that the probability of this cluster occurring in the volume sampled by the EMSS is less than a few times 10^{-5}. Nor is MS1054-0321 the only hot cluster at high redshift; the only two other $z > 0.5$ EMSS clusters already observed with ASCA also have temperatures exceeding 8 keV. Assuming again that the initial perturbations were Gaussian and Omega_0 = 1, we find that each one is improbable at the < 10^{-2} level. These observations, along with the fact that these luminosities and temperatures of the high-$z$ clusters all agree with the low-z L_X-T_X relation, argue strongly that Omega_0 < 1. Otherwise, the initial perturbations must be non-Gaussian, if these clusters' temperatures do indeed reflect their gravitational potentials.Comment: 20 pages, 4 figures, To appear in 1 Aug 1998 ApJ (heavily revised version of original preprint

The slope of the black-hole mass versus velocity dispersion correlation

Observations of nearby galaxies reveal a strong correlation between the mass of the central dark object M and the velocity dispersion sigma of the host galaxy, of the form log(M/M_sun) = a + b*log(sigma/sigma_0); however, published estimates of the slope b span a wide range (3.75 to 5.3). Merritt & Ferrarese have argued that low slopes (<4) arise because of neglect of random measurement errors in the dispersions and an incorrect choice for the dispersion of the Milky Way Galaxy. We show that these explanations account for at most a small part of the slope range. Instead, the range of slopes arises mostly because of systematic differences in the velocity dispersions used by different groups for the same galaxies. The origin of these differences remains unclear, but we suggest that one significant component of the difference results from Ferrarese & Merritt's extrapolation of central velocity dispersions to r_e/8 (r_e is the effective radius) using an empirical formula. Another component may arise from dispersion-dependent systematic errors in the measurements. A new determination of the slope using 31 galaxies yields b=4.02 +/- 0.32, a=8.13 +/- 0.06, for sigma_0=200 km/s. The M-sigma relation has an intrinsic dispersion in log M that is no larger than 0.3 dex. In an Appendix, we present a simple model for the velocity-dispersion profile of the Galactic bulge.Comment: 37 pages, 9 figure

The ACS Virgo Cluster Survey. XIV. Analysis of Color-Magnitude Relations in Globular Cluster Systems

We examine the correlation between globular cluster (GC) color and magnitude using HST/ACS imaging for a sample of 79 early-type galaxies (-21.7<M_B<-15.2 mag) with accurate SBF distances from the ACS Virgo Cluster Survey. Using the KMM mixture modeling algorithm, we find a highly significant correlation, d(g-z)/dz = -0.037 +- 0.004, between color and magnitude for the subpopulation of blue GCs in the co-added GC color-magnitude diagram of the three brightest Virgo galaxies (M49, M87 and M60): brighter GCs are redder than their fainter counterparts. For the single GC systems of M87 and M60, we find similar correlations; M49 does not appear to show a significant trend. There is no correlation between (g-z) and M_z for GCs of the red subpopulation. The correlation d(g-z)/dg for the blue subpopulation is much weaker than d(g-z)/dz. Using Monte Carlo simulations, we attribute this to the fact that the blue subpopulation in M_g extends to higher luminosities than the red subpopulation, which biases the KMM fits. The correlation between color and M_z thus is a real effect. This conclusion is supported by biweight fits to the same color distributions. We identify two environmental dependencies of the color-magnitude relation: (1) the slope decreases in significance with decreasing galaxy luminosity; and (2) the slope is stronger for GCs at smaller galactocentric distances. We examine several mechanisms that might give rise to the observed color-magnitude relation: (1) presence of contaminators; (2) accretion of GCs from low-mass galaxies; (3) stochastic effects; (4) capture of field stars by individual GCs; and (5) GC self-enrichment. We conclude that self-enrichment and field-star capture, or a combination of these processes, offer the most promising means of explaining our observations.Comment: 15 pages, 12 figures, accepted for publication in the Astrophysical Journal. Uses emulateapj.cl

A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes

Restriction Modification (RM) systems prevent the invasion of foreign genetic material into bacterial cells by restriction and protect the host's genetic material by methylation. They are therefore important in maintaining the integrity of the host genome. RM systems are currently classified into four types (I to IV) on the basis of differences in composition, target recognition, cofactors and the manner in which they cleave DNA. Comparing the structures of the different types, similarities can be observed suggesting an evolutionary link between these different types. This work describes the ‘deconstruction’ of a large Type I RM enzyme into forms structurally similar to smaller Type II RM enzymes in an effort to elucidate the pathway taken by Nature to form these different RM enzymes. Based upon the ability to engineer new enzymes from the Type I ‘scaffold’, an evolutionary pathway and the evolutionary pressures required to move along the pathway from Type I RM systems to Type II RM systems are proposed. Experiments to test the evolutionary model are discussed

The Demography of Massive Dark Objects in Galaxy Centres

We construct dynamical models for a sample of 36 nearby galaxies with Hubble Space Telescope photometry and ground-based kinematics. The models assume that each galaxy is axisymmetric, with a two-integral distribution function, arbitrary inclination angle, a position-independent stellar mass-to-light ratio Upsilon, and a central massive dark object (MDO) of arbitrary mass M_bh. They provide acceptable fits to 32 of the galaxies for some value of M_bh and Upsilon; the four galaxies that cannot be fit have kinematically decoupled cores. The mass-to-light ratios inferred for the 32 well-fit galaxies are consistent with the fundamental plane correlation Upsilon \propto L^0.2, where L is galaxy luminosity. In all but six galaxies the models require at the 95% confidence level an MDO of mass M_bh ~ 0.006 M_bulge = 0.006 Upsilon L. Five of the six galaxies consistent with M_bh=0 are also consistent with this correlation. The other (NGC 7332) has a much stronger upper limit on M_bh. We consider various parameterizations for the probability distribution describing the correlation of the masses of these MDOs with other galaxy properties. One of the best models can be summarized thus: a fraction f ~0.97 of galaxies have MDOs, whose masses are well described by a Gaussian distribution in log (M_bh/M_bulge) of mean -2.27 and width ~0.07.Comment: 28 pages including 13 figures and 4 tables. Submitted to A

A directed enolization strategy enables by-product-free construction of contiguous stereocentres en route to complex amino acids.

Homochiral α-amino acids are widely used in pharmaceutical design as key subunits in chiral catalyst synthesis or as building blocks in synthetic biology. Many synthetic methods have been developed to access rare or unnatural variants by controlling the installation of the α-stereocentre. By contrast, and despite their importance, α-amino acids possessing β-stereocentres are much harder to synthesize. Here we demonstrate an iridium-catalysed protocol that allows the direct upconversion of simple alkenes and glycine derivatives to give β-substituted α-amino acids with exceptional levels of regio- and stereocontrol. Our method exploits the native directing ability of a glycine-derived N-H unit to facilitate Ir-catalysed enolization of the adjacent carbonyl. The resulting stereodefined enolate cross-couples with a styrene or α-olefin to install two contiguous stereocentres. The process offers very high levels of regio- and stereocontrol and occurs with complete atom economy. In broader terms, our reaction design offers a unique directing-group-controlled strategy for the direct stereocontrolled α-alkylation of carbonyl compounds, and provides a powerful approach for the synthesis of challenging contiguous stereocentres