A simple and rapid nucleic acid preparation method for reverse transcription polymerase chain reaction (RTPCR) in dormant potato tubers

In order to shorten and facilitate the preparation of nucleic acid (without using tuber slicer, santurugation, vacuum devices and nanocalorimeter (NCM)) for reverse transcription polymerase chain reaction (RT-PCR), pieces of tuber were placed directly into eppendorf tubes containing 30 ìl ofdetergent (0.5% Triton XL-80 N) buffers, vortexed for 3 min and then 2.5 ìl of this aliquots was used in RT-PCR. Alternatively, evaluation of the efficiency of different buffers (sodium sulphite buffer, 0.1 M HCl, 0.1 M NaOH, 0.1 M Tris, 0.1 M EDTA, 0.1 M phosphate buffer, 0.1 M LiCl, 0.1 M KCl, 1 M NaCl and water (H2O)) was carried out. The strongest band was obtained from sodium chloride (NaCl), potassium chloride (KCl), sodium sulphite buffer (SSB), phosphate buffer and lithium chloride (LiCl), respectively. To determine the minimum concentration and delectability of potato leafroll virus (PLRV) and potato virus S (PVS) that can be assessed by RT-PCR, the sap obtained from tubers known to be infected withPLRV and PVS were diluted (1:1, 1:2, 1: 4, 1:8, 1:16 1:32 and 1:64) and tested in RT-PCR. PLRV and PVS bands were observed up to 1:8 in NaCl and phosphate buffer, 1:4 in KCl, SSB and LiCl. However, exceptNaCl, no difference between the intensity of band obtained from other buffers was observed. On the other hand, increasing the cut surfaces of tuber pieces increased the dilution rate of nucleic acid. To determine the efficiency of the NaCl buffer, when a total of 60 dormant tubers (60 days after the harvest) were tested, PLRV and PVS were detected at the rate of 57/60 and 54/60, respectively

Ku Band Rotary Joint Design for SNG Vehicles

A wideband I-type rectangular waveguide rotary joint (RJ) is designed, simulated and built. It has an excellent performance over the whole Ku Band (10.7-14.5 GHz) where the return loss is less than -23 dB at its highest and the insertion loss is below 0.4 dB. The rotary joint is specifically designed for satellite news gathering (SNG) vehicles providing elevation and azimuthal movement to the antenna and matching polarization when it is needed at the feed. It can also be used in other high power microwave applications,where rotation ability of the antenna is a must during the transmission such as radars

Occurrence of Pospiviroid in potato, tomato and some ornamental plants in Turkey

In order to investigate the Pospiviroid in potato, tomato and some ornamental plants in Turkey, a survey study was carried out from 2006 to 2009. During this survey study, a total of 1766 samples (1047potato samples, 258 tomato leaves and 461 ornamental plants) were tested by reverse transcriptionpolymerase chain reaction (RT-PCR) and, the samples found to be positive in RT-PCR were also checked in return polyacrylamide gel electrophoresis (R-PAGE). Of these samples, potato tubers were collected from markets, leaf samples from potato fields (Erzurum), tomato samples from field (Tokat, Amasya, Balýkesir and Bursa) and greenhouses (Antalya and Mula), leaves and vines of ornamental plants from greenhouses (Yalova) and florists (Erzurum) and recreation areas (Ankara, zmir and Trabzon). In the result of RT-PCR test, PSTVd was detected in 6 out of 891 potato tubers and CSVd in 2out of 154 chrysanthemums. On the other side, tomato, citrus and most ornamental plants belonging to different families were found to be free from pospiviroid. This is the first report of CSVd in Turkey

Half-life of the I Π = 4 - intruder state in 34 P: M 2 transition strengths approaching the island of inversion

The half-life of the 2305-keV, I?=4 (-) intruder state in 34P has been measured as t 1/2=2.0(1) ns using ?-ray coincidence, fast-timing techniques with the Bucharest HPGe and LaBr 3:Ce detector array. Excited states in 34P were populated using the 18O (18O,pn )34P fusion-evaporation reaction at a beam energy of 36 MeV. Reduced transition probabilities have been calculated for different values of the M2/E3 mixing ratio, ? E3/M2, and compared with shell-model estimates. For small values of the mixing ratio, the B(M2) value was found to be consistent with similar transitions associated with the occupation of neutron f 7/2 configurations in this mass region. � 2012 American Physical Society

Holonomic functions of several complex variables and singularities of anisotropic Ising n-fold integrals

Lattice statistical mechanics, often provides a natural (holonomic) framework to perform singularity analysis with several complex variables that would, in a general mathematical framework, be too complex, or could not be defined. Considering several Picard-Fuchs systems of two-variables "above" Calabi-Yau ODEs, associated with double hypergeometric series, we show that holonomic functions are actually a good framework for actually finding the singular manifolds. We, then, analyse the singular algebraic varieties of the n-fold integrals $\chi^{(n)}$, corresponding to the decomposition of the magnetic susceptibility of the anisotropic square Ising model. We revisit a set of Nickelian singularities that turns out to be a two-parameter family of elliptic curves. We then find a first set of non-Nickelian singularities for $\chi^{(3)}$ and $\chi^{(4)}$, that also turns out to be rational or ellipic curves. We underline the fact that these singular curves depend on the anisotropy of the Ising model. We address, from a birational viewpoint, the emergence of families of elliptic curves, and of Calabi-Yau manifolds on such problems. We discuss the accumulation of these singular curves for the non-holonomic anisotropic full susceptibility.Comment: 36 page

New Results for the Correlation Functions of the Ising Model and the Transverse Ising Chain

In this paper we show how an infinite system of coupled Toda-type nonlinear differential equations derived by one of us can be used efficiently to calculate the time-dependent pair-correlations in the Ising chain in a transverse field. The results are seen to match extremely well long large-time asymptotic expansions newly derived here. For our initial conditions we use new long asymptotic expansions for the equal-time pair correlation functions of the transverse Ising chain, extending an old result of T.T. Wu for the 2d Ising model. Using this one can also study the equal-time wavevector-dependent correlation function of the quantum chain, a.k.a. the q-dependent diagonal susceptibility in the 2d Ising model, in great detail with very little computational effort.Comment: LaTeX 2e, 31 pages, 8 figures (16 eps files). vs2: Two references added and minor changes of style. vs3: Corrections made and reference adde

Meta-analysis: COVID-19 diagnosis in chest CT�master key for radiologists

Background: COVID-19 was discovered in February in China. Due to the high prevalence of the disease, early detection and rapid isolation of patients are the vital points for controlling the outbreak. The purpose of this study was to determine the correct location of chest CT scan in the diagnosis of COVID-19. Main text: The current study is a systematic review and meta-analysis. 2959 papers were found in all national and international databases. The study has been reported based on the PRISMA checklist. All analyses were done by CMA Ver. 2 software. The statistical analysis results show that the GGO observation level in the available shape was 46 in CT scan results, and the consolidation observation level in the general form was 33 in CT scan results. Pleural effusion was 7, and linear opacity observation level was 24 in CT scan results in the general form. The CT scan test sensitivity level was gained 94.7, and PCR test sensitivity level was achieved as 94.8. This level was 89 in the early stage. Conclusion: The chest CT has about 24 higher diagnostic sensitivity than the PCR test, in the early stage. GGO revealed a declining process and also indicates that GGO is an early symptom of the disease in CT scan. Linear opacity is the reason behind the initial dyspnea in coronavirus suffering patients referring to the medical centers. The extra-pulmonary lesions increase in the last stage of the disease that makes the patient�s worse. © 2021, The Author(s)