On the Cahn-Hilliard-Brinkman system

We consider a diffuse interface model for phase separation of an isothermal incompressible binary fluid in a Brinkman porous medium. The coupled system consists of a convective Cahn-Hilliard equation for the phase field $\phi$, i.e., the difference of the (relative) concentrations of the two phases, coupled with a modified Darcy equation proposed by H.C. Brinkman in 1947 for the fluid velocity $\mathbf{u}$. This equation incorporates a diffuse interface surface force proportional to $\phi \nabla \mu$, where $\mu$ is the so-called chemical potential. We analyze the well-posedness of the resulting Cahn-Hilliard-Brinkman (CHB) system for $(\phi,\mathbf{u})$. Then we establish the existence of a global attractor and the convergence of a given (weak) solution to a single equilibrium via {\L}ojasiewicz-Simon inequality. Furthermore, we study the behavior of the solutions as the viscosity goes to zero, that is, when the CHB system approaches the Cahn-Hilliard-Hele-Shaw (CHHS) system. We first prove the existence of a weak solution to the CHHS system as limit of CHB solutions. Then, in dimension two, we estimate the difference of the solutions to CHB and CHHS systems in terms of the viscosity constant appearing in CHB

Systematic numerical investigation of the role of hierarchy in heterogeneous bio-inspired materials

It is well known that hierarchical structure is an important feature in biological materials to optimise various properties, including mechanical ones. It is however still unclear how these hierarchical architectures can improve material characteristics, for example strength. Also, the transposition of these structures from natural to artificial bioinspired materials remains to be perfected. In this paper, we introduce a numerical method to evaluate the strength of fibre-based heterogeneous biological materials and systematically investigate the role of hierarchy. Results show that hierarchy indeed plays an important role and that it is possible to “tune” the strength of bio-inspired materials in a wide range of values, in some cases improving the strength of non-hierarchical structures considerably

Gene autoregulation via intronic microRNAs and its functions

Background: MicroRNAs, post-transcriptional repressors of gene expression, play a pivotal role in gene regulatory networks. They are involved in core cellular processes and their dysregulation is associated to a broad range of human diseases. This paper focus on a minimal microRNA-mediated regulatory circuit, in which a protein-coding gene (host gene) is targeted by a microRNA located inside one of its introns. Results: Autoregulation via intronic microRNAs is widespread in the human regulatory network, as confirmed by our bioinformatic analysis, and can perform several regulatory tasks despite its simple topology. Our analysis, based on analytical calculations and simulations, indicates that this circuitry alters the dynamics of the host gene expression, can induce complex responses implementing adaptation and Weber's law, and efficiently filters fluctuations propagating from the upstream network to the host gene. A fine-tuning of the circuit parameters can optimize each of these functions. Interestingly, they are all related to gene expression homeostasis, in agreement with the increasing evidence suggesting a role of microRNA regulation in conferring robustness to biological processes. In addition to model analysis, we present a list of bioinformatically predicted candidate circuits in human for future experimental tests. Conclusions: The results presented here suggest a potentially relevant functional role for negative self-regulation via intronic microRNAs, in particular as a homeostatic control mechanism of gene expression. Moreover, the map of circuit functions in terms of experimentally measurable parameters, resulting from our analysis, can be a useful guideline for possible applications in synthetic biology.Comment: 29 pages and 7 figures in the main text, 18 pages of Supporting Informatio

A combination of transcriptional and microRNA regulation improves the stability of the relative concentrations of target genes

It is well known that, under suitable conditions, microRNAs are able to fine tune the relative concentration of their targets to any desired value. We show that this function is particularly effective when one of the targets is a Transcription Factor (TF) which regulates the other targets. This combination defines a new class of feed-forward loops (FFLs) in which the microRNA plays the role of master regulator. Using both deterministic and stochastic equations we show that these FFLs are indeed able not only to fine-tune the TF/target ratio to any desired value as a function of the miRNA concentration but also, thanks to the peculiar topology of the circuit, to ensures the stability of this ratio against stochastic fluctuations. These two effects are due to the interplay between the direct transcriptional regulation and the indirect TF/Target interaction due to competition of TF and target for miRNA binding (the so called "sponge effect"). We then perform a genome wide search of these FFLs in the human regulatory network and show that they are characterizedby a very peculiar enrichment pattern. In particular they are strongly enriched in all the situations in which the TF and its target have to be precisely kept at the same concentration notwithstanding the environmental noise. As an example we discuss the FFL involving E2F1 as Transcription Factor, RB1 as target and miR-17 family as master regulator. These FFLs ensure a tight control of the E2F/RB ratio which in turns ensures the stability of the transition from the G0/G1 to the S phase in quiescent cells.Comment: 23 pages, 10 figure

Leonardo Mosso: the poetry of structure

Leonardo Mosso (1926-2020) was able to combine art and architecture. He was a collector and an interpreter of 20th century culture that he shared and passed on to succeeding generations of students and collaborators who attended the Alvar Aalto Institute. For many, he was an unsung Maestro, a generous polymath, who maintained an extraordinary curiosity and child-like enthusiasm throughout his long life

Progetto locale ecocompatibile di filiera di prodotto:pannelli isolanti innovativi in lana di pecora. A sustainable building product: advanced insulation panels obtained by recycling regional sheep's wool

The article deal with an ongoing research aimed at developing an advanced self-bearing panel, fitted for thermal and acoustic insulation of buildings, derived from the reuse and recycling of local sheep wool. The development of a supply chain of environmentally friendly products (a self bearing panel made of 100% wool) encourages, on the one hand, the use of a material so far classified in Italy as special waste and, on the one other, provides new opportunities for a sheep farming that it is now going through an economic recession, with positive effects on the mountain and the hill landscap