Direct target and non-target analysis of urban aerosol sample extracts using atmospheric pressure photoionisation high-resolution mass spectrometry

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous atmospheric pollutants of high concern for public health. In the atmosphere they undergo oxidation, mainly through reactions with center dot OH and NOx to produce nitro- and oxygenated (oxy-) derivatives. In this study, we developed a new method for the detection of particle-bound PAHs, nitro-PAHs and oxy-PAHs using direct infusion into an atmospheric pressure photoionisation high-resolution mass spectrometer (APPI-HRMS). Method optimisation was done by testing different source temperatures, gas flow rates, mobile phases and dopants. Samples were extracted with methanol, concentrated by evaporation and directly infused in the APPI source after adding toluene as dopant. Acquisition was performed in both polarity modes. The method was applied to target analysis of seasonal PM2.5 samples from an urban background site in Padua (Italy), in the Po Valley, in which a series of PAHs, nitro- and oxy-PAHs were detected. APPI-HRMS was then used for non-target analysis of seasonal PM2.5 samples and results compared with nano-electrospray ionisation (nanoESI) HRMS. The results showed that, when samples were characterised by highly oxidised organic compounds, including S-containing compounds, like in summer samples, APPI did not bring any additional information with respect to nanoESI in negative polarity (nanoESI(-)). Conversely, for winter samples, APPI(-) could detect a series of aromatic and poly-aromatic compounds, mainly oxidised and nitrogenated aromatics, that were not otherwise detected with nanoESI. (C) 2019 Elsevier Ltd. All rights reserved

ENVIRONMENTAL ASSESSMENT OF AN INNOVATIVE PLANT FOR THE WASTEWATER PURIFICATION IN THE BEVERAGE INDUSTRY

Nowadays, efforts to reduce the resource depletion and environmental emissions from the anthropic activities, are mandatory for sustainable development pattern. Among the key resources to save, pure water is as important as critic due to its scarcity and its essential role for life and growth. Furthermore, during the last decades, rising attention from institutions and industries is toward solutions for the water intensity decrease and wastewater recovery. This paper proposes the environmental assessment of an innovative wastewater collection and purification plant tailored to a mid-size beverage industry aiming at locally closing the loop of the water chain, allowing its recirculation and local reuse. After the description of the functional module features, sizes and design, based on a prototype actually working in Italy, the paper follows the ISO 14040 standards to develop an environmental assessment of the industrial system, quantifying the impact rising from the manufacturing and the assembly phases

Uso do ácido indol butírico na estaquia de Tibouchina sellowiana (Cham.) Cogn. no outono e inverno de 2004.

Resumo

Tibouchina sellowiana (Cham.) Cogn.: enraizamento, anatomia e análises bioquímicas nas quatro estações do ano.

O presente trabalho buscou verificar os efeitos da aplicação de diferentes concentrações do ácido indolbutírico (AIB), em talco e em solução, no enraizamento de estacas caulinares de Tibouchina sellowiana (Cham.) Cogn. coletadas nas quatro estações do ano, bem como suas variações anatômicas e bioquímicas. Estacas semilenhosas foram tratadas com 0, 1500 e 3000 mg L-1 de ácido indolbutírico (AIB), em talco e solução 50% alcoólica. Após 65 dias em casa-de-vegetação, foram avaliados a porcentagem de estacas enraizadas, o número e o comprimento das raízes, a porcentagem de estacas com calos e a sobrevivência. O uso do AIB mostrou-se eficiente na indução radicial, sendo indiferente a forma em que foi aplicado. As estações mais promissoras foram a primavera (78,75%) com 3.000 mg L-1 ou mg kg-1 de AIB e o verão (63,12%) com 1.500 mg L-1 ou mg kg-1 de AIB. Das secções feitas na base das estacas, no momento da estaquia, não foi detectada nenhuma possível barreira anatômica ou diferença estrutural que promovesse variações no enraizamento. Análises bioquímicas feitas das estacas revelaram que as maiores concentrações de açúcares totais foram obtidas no inverno (83,21 mg g-1 de tecido) e no outono (72,79 mg g-1), as estações que também apresentaram as maiores concentrações de proteínas (4,69 e 3,95 mg g-1 respectivamente)

Promising Non-cytotoxic Monosubstituted Chalcones to Target Monoamine Oxidase-B

A library of monosubstituted chalcones (1-17) bearing electron-donating and electron-withdrawing groups on both aromatic rings were selected. The cell viability on human tumor cell lines was evaluated first. The compounds unable to induce detectable cytotoxicity (1, 13, and 14) were tested using the monoamine oxidase (MAO) activity assay. Interestingly, they inhibit MAO-B, acting as competitive inhibitors, with 13 and 14 showing the best profiles. In particular, 13 exhibited a potency higher than that of safinamide, taken as a reference. Docking studies and crystallographic analysis showed that in human MAO-B 13 binds with the halogen-substituted aromatic ring in the entrance cavity, similar to safinamide, whereas 14 is accommodated in the opposite way. The main conclusion of this cell biology, biochemistry, and structural study is to highlights 13 as a chalcone derivative that is worth consideration for the development of novel MAO-B-selective inhibitors for the treatment of neurodegenerative diseases

A MULTICENTER, OBSERVATIONAL, AMBISPECTIVE STUDY EVALUATING EFFICACY AND SAFETY OF GENERIC IMATINIB COMPARED TO GLEEVEC IN CHRONIC MYELOGENOUS LEUKEMIA IN CHRONIC PHASE-3 MONTHS RESPONSE ANALYSIS

Univ Estadual Campinas, Hematol & Hemotherapy Ctr, Campinas, SP, BrazilUniv Fed Minas Gerais, Hosp Clin, Belo Horizonte, MG, BrazilInst Nacl Cancer, Rio De Janeiro, BrazilFac Med, Sao Paulo, BrazilUniv Sao Paulo, Sao Paulo, BrazilHemorio, Rio De Janeiro, BrazilHosp Clin Porto Alegre, Porto Alegre, RS, BrazilCtr Pesquisa Oncol Santa Catarina, Florianopolis, SC, BrazilUniv Fed Bahia, Salvador, BA, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilInst Estudos & Pesquisas Sao Lucas, Sao Paulo, BrazilUniv Estadual Campinas, Campinas, SP, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilWeb of Scienc