150 research outputs found
Programming of enzyme specificity by substrate mimetics: investigations on the Glu-specific V8 protease reveals a novel general principle of biocatalysis1Dedicated to Prof. Dr. H.-D. Jakubke on the occasion of his 65th birthday.1
AbstractIn this paper the universal validity of the substrate mimetic concept in enzymatic C-N ligations was expanded to anionic leaving groups based on the specificity determinants of Glu-specific endopeptidase from Staphylococcus aureus (V8 protease). In an empirical way a specific mimetic moiety was designed from simple structure-function relationship studies. The general function of the newly developed substrate mimetics to serve as an artificial recognition site for V8 protease have been examined by hydrolysis kinetic studies. Enzymatic peptide syntheses qualify the strategy of substrate mimetics as a powerful concept for programming the enzyme specificity in the direction of a more universal application of enzymes in the general area of biocatalysis
Nonconventional amide bond formation catalysis: programming enzyme specificity with substrate mimetics
Computer-Aided Solvent Screening for Biocatalysis
A computer-aidedsolventscreening methodology is described and tested for biocatalytic systems composed of enzyme, essential water and substrates/products dissolved in a solvent medium, without cells. The methodology is computationally simple, using group contribution methods for calculating constrained properties related to chemical reaction equilibrium, substrate and product solubility, water solubility, boiling points, toxicity and others. Two examples are provided, covering the screening of solvents for lipase-catalyzed transesterification of octanol and inulin with vinyl laurate. Esterification of acrylic acid with octanol is also addressed. Solvents are screened and candidates identified, confirming existing experimental results. Although the examples involve lipases, the method is quite general, so there seems to be no preclusion against application to other biocatalyst
Studies on the formation and synthetic mechanism of related substance G in potassium clavulanate production
Substrate mimetics in protease catalysis: Characteristics, kinetics, and synthetic utility
ChemInform Abstract: Protease-Catalyzed Peptide Synthesis from N- to C-Terminus: An Advantageous Strategy.
ChemInform Abstract: Nonconventional Amide Bond Formation Catalysis: Programming Enzyme Specificity with Substrate Mimetics
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