Gaseous argon time projection chamber with electroluminescence enhanced optical readout

Systematic uncertainties in accelerator oscillation neutrino experiments arise mostly from nuclear models describing neutrino-nucleus interactions. To mitigate these uncertainties, we can study neutrino-nuclei interactions with detectors possessing enhanced hadron detection capabilities at energies below the nuclear Fermi level. Gaseous detectors not only lower the particle detection threshold but also enable the investigation of nuclear effects on various nuclei by allowing for changes in the gas composition. This approach provides valuable insights into the modelling of neutrino-nucleus interactions and significantly reduces associated uncertainties. Here, we discuss the design and first operation of a gaseous argon time projection chamber optically read. The detector operates at atmospheric pressure and features a single stage of electron amplification based on a thick GEM. Here, photons are produced with wavelengths in the vacuum ultraviolet regime. In an optical detector the primary constraint is the light yield. This study explores the possibility of increasing the light yield by applying a low electric field downstream of the ThGEM. In this region, called the electroluminescence gap, electrons propagate and excite the argon atoms, leading to the subsequent emission of photons. This process occurs without any further electron amplification, and it is demonstrated that the total light yield increases up to three times by applying moderate electric fields of the order of 3~kV/cm. Finally, an indirect method is discussed for determining the photon yield per charge gain of a ThGEM, giving a value of 18.3 photons detected per secondary electron

Insight on Glucose and Fructose Absorption and Relevance in the Enterocyte Milieu

Although epidemiological studies indicate a strong correlation between high sugar intake and metabolic diseases, the biological mechanisms underlying this link are still controversial. To further examine the modification and crosstalk occurring in enterocyte metabolism during sugar absorption, in this study we evaluate the diffusion and intestinal metabolism of glucose, fructose and sucrose, which were supplemented in equimolar concentration to Caco-2 cells grown on polyester membrane inserts. At different time points after supplementation, changes in metabolite concentration were evaluated in the apical and basolateral chambers by nuclear magnetic resonance (NMR) and gas-chromatography (GC). Sucrose was only minimally hydrolyzed by Caco-2 cells. Upon supplementation, we observed a faster uptake of fructose than glucose, the pentose sugar being also faster catabolized. Monosaccharide absorption was concomitant to the synthesis/transport of other metabolites, which occurred differently in glucose and fructose supplemented cells. Our results confirm the prominent role of intestinal cells in fructose metabolism and clearance after absorption, representing a further step forward in the understanding of the role of dietary sugars. Future research, including targeted analysis on specific transporters/enzymes and the use of labeled substrates, will be helpful to confirm the present results and their interpretation

Improving spatial landslide prediction with 3d slope stability analysis and genetic algorithm optimization: Application to the oltrepò pavese

In this study, we compare infinite slope and the three-dimensional stability analysis performed by SCOOPS 3D (software to analyze three-dimensional slope stability throughout a digital landscape). SCOOPS 3D is a model proposed by the U. S. Geological Survey (USGS), the potentialities of which have still not been investigated sufficiently. The comparison between infinite slope and 3D slope stability analysis is carried out using the same hydrological analysis, which is performed with TRIGRS (transient rainfall infiltration and grid-based regional slope-stability model)—another model proposed by USGS. The SCOOPS 3D model requires definition of a series of numerical parameters that can have a significant impact on its own performance, for a given set of physical properties. In the study, we calibrate these numerical parameters through a multi-objective optimization based on genetic algorithms to maximize the model predictability performance in terms of statistics of the receiver operating characteristics (ROC) confusion matrix. This comparison is carried out through an application on a real case study, a catchment in the Oltrepò Pavese (Italy), in which the areas of triggered landslides were accurately monitored during an extreme rainfall on 27–28 April 2009. Results show that the SCOOPS 3D model performs better than the 1D infinite slope stability analysis, as the ROC True Skill Statistic increases from 0.09 to 0.37. In comparison to other studies, we find the 1D model performs worse, likely for the availability of less detailed geological data. On the other side, for the 3D model we find even better results than the two other studies present to date in the scientific literature. This is to be attributed to the optimization process we proposed, which allows to have a greater gain of performance passing from the 1D to the 3D simulation, in comparison to the above-mentioned studies, where no optimization has been applied. Thus, our study contributes to improving the performances of landslide models, which still remain subject to many uncertainty factors

JCV-specific T-cells producing IFN-gamma are differently associated with PmL occurrence in HIV patients and liver transplant recipients

Aim of this work was to investigate a possible correlation between the frequency of JCV-specific T-cells and PML occurrence in HIV-infected subjects and in liver transplant recipients. A significant decrease of JCV-specific T-cells was observed in HIV-PML subjects, highlighting a close relation between JCV-specific T-cell immune impairment and PML occurrence in HIV-subjects. Interestingly, liver-transplant recipients (LTR) showed a low frequency of JCV-specific T-cells, similar to HIV-PML subjects. Nevertheless, none of the enrolled LTR developed PML, suggesting the existence of different immunological mechanisms involved in the maintenance of a protective immune response in LT

Malonic acid: A potential reagent in decontamination processes for Ni-rich alloy surfaces

The ability of malonic acid as a dissolution agent toward synthetic Ni ferrite and Alloy 600 and 800 corrosion products was explored. Its performance in the dissolution kinetics of Ni ferrite powders was compared with the one of oxalic acid. Kinetic parameters were obtained and the dependency on external Fe(II) was modelled. Oxidized samples used in descaling tests were prepared by exposure of coupons of both alloys to lithiated aqueous solutions, under hydrothermal conditions and hydrogen overpressure, simulating PHWR conditions. Oxide layer morphology, the influence of exposure time to corrosive medium and LiOH concentration on its thickness were characterized. Descaling tests consisting on a two-stage method (a first oxidizing step with alkaline permanganate followed by a reducing step with oxalic or malonic acid were carried out). Results were compared to those obtained with a well known chemical cleaning formulation (APAC: Alkaline Permanganate Ammonium Citrate) used in decontamination of several reactors and loops and the competitiveness of malonic acid was demonstrated.Fil: García, D.. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; ArgentinaFil: Bruyre, V. I. E.. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; ArgentinaFil: Bordoni, R.. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaFil: Olmedo, A. M.. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaFil: Morando, Pedro Juan. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Biomaterials in Neurodegenerative Disorders : A Promising Therapeutic Approach

Neurodegenerative disorders (i.e., Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and spinal cord injury) represent a great problem worldwide and are becoming prevalent because of the increasing average age of the population. Despite many studies having focused on their etiopathology, the exact cause of these diseases is still unknown and until now, there are only symptomatic treatments. Biomaterials have become important not only for the study of disease pathogenesis, but also for their application in regenerative medicine. The great advantages provided by biomaterials are their ability to mimic the environment of the extracellular matrix and to allow the growth of different types of cells. Biomaterials can be used as supporting material for cell proliferation to be transplanted and as vectors to deliver many active molecules for the treatments of neurodegenerative disorders. In this review, we aim to report the potentiality of biomaterials (i.e., hydrogels, nanoparticles, self-assembling peptides, nanofibers and carbon-based nanomaterials) by analyzing their use in the regeneration of neural and glial cells their role in axon outgrowth. Although further studies are needed for their use in humans, the promising results obtained by several groups leads us to suppose that biomaterials represent a potential therapeutic approach for the treatments of neurodegenerative disorders

Olive oil by-product as functional ingredient in bakery products. Influence of processing and evaluation of biological effects

Nowadays, the strong demand for adequate nutrition is accompanied by concern about environmental pollution and there is a considerable emphasis on the recovery and recycling of food by-products and wastes. In this study, we focused on the exploitation of olive pomace as functional ingredient in biscuits and bread. Standard and enriched bakery products were made using different flours and fermentation protocols. After characterization, they were in vitro digested and used for supplementation of intestinal cells (Caco-2), which underwent exogenous inflammation. The enrichment caused a significant increase in the phenolic content in all products, particularly in the sourdough fermented ones. Sourdough fermentation also increased tocol concentration. The increased concentration of bioactive molecules did not reflect the anti-inflammatory effect, which was modulated by the baking procedure. Conventionally fermented bread enriched with 4% pomace and sourdough fermented, not-enriched bread had the greatest anti-inflammatory effect, significantly reducing IL-8 secretion in Caco-2 cells. The cell metabolome was modified only after supplementation with sourdough fermented bread enriched with 4% pomace, probably due to the high concentration of tocopherol that acted synergistically with polyphenols. Our data highlight that changes in chemical composition cannot predict changes in functionality. It is conceivable that matrices (including enrichment) and processing differently modulated bioactive bioaccessibility, and consequently functionality

Synthesis of novel structural hybrids between aza-heterocycles and azelaic acid moiety with a specific activity on osteosarcoma cells

Nine compounds bearing pyridinyl (or piperidinyl, benzimidazolyl, benzotriazolyl) groups bound to an azelayl moiety through an amide bond were synthesized. The structural analogy with some histone deacetylase inhibitors inspired their syntheses, seeking new selective histone deacetylase inhibitors (HDACi). The azelayl moiety recalls part of 9-hydroxystearic acid, a cellular lipid showing antiproliferative activity toward cancer cells with HDAC as a molecular target. Azelayl derivatives bound to a benzothiazolyl moiety further proved to be active as HDACi. The novel compounds were tested on a panel of both normal and tumor cell lines. Non-specific induction of cytotoxicity was observed in the normal cell line, while three of them induced a biological effect only on the osteosarcoma (U2OS) cell line. One of them induced a change in nuclear shape and size. Cell-cycle alterations are associated with post-transcriptional modification of both H2/H3 and H4 histones. In line with recent studies, revealing unexpected HDAC7 function in osteoclasts, molecular docking studies on the active molecules predicted their proneness to interact with HDAC7. By reducing side effects associated with the action of the first-generation inhibitors, the herein reported compounds, thus, sound promising as selective HDACi

Genome sequencing of Prototheca zopfii genotypes 1 and 2 provides evidence of a severe reduction in organellar genomes

Abstract Prototheca zopfii (P. zopfii, class Trebouxiophyceae, order Chlorellales, family Chlorellaceae), a non-photosynthetic predominantly free-living unicellular alga, is one of the few pathogens belonging to the plant kingdom. This alga can affect many vertebrate hosts, sustaining systemic infections and diseases such as mastitis in cows. The aim of our work was to sequence and assemble the P. zopfii genotype 1 and genotype 2 mitochondrial and plastid genomes. Remarkably, the P. zopfii mitochondrial (38 Kb) and plastid (28 Kb) genomes are models of compaction and the smallest known in the Trebouxiophyceae. As expected, the P. zopfii genotype 1 and 2 plastid genomes lack all the genes involved in photosynthesis, but, surprisingly, they also lack those coding for RNA polymerases. Our results showed that plastid genes are actively transcribed in P. zopfii, which suggests that the missing RNA polymerases are substituted by nuclear-encoded paralogs. The simplified architecture and highly-reduced gene complement of the P. zopfii mitochondrial and plastid genomes are closer to those of P. stagnora and the achlorophyllous obligate parasite Helicosporidium than to those of P. wickerhamii or P. cutis. This similarity is also supported by maximum likelihood phylogenetic analyses inferences. Overall, the P. zopfii sequences reported here, which include nuclear genome drafts for both genotypes, will help provide both a deeper understanding of the evolution of Prototheca spp. and insights into the corresponding host/pathogen interactions