71 research outputs found
The consequences of the effects of the chemotherapeutic drug (vincristine) in organs and the influence on the bioavailability of two radio-biocomplexes used for bone evaluations in balb/c female mice
The development of animal model to evaluate the toxicological action of compounds used as pharmaceutical drugs is desired. The model described in this work is based on the capability of drugs to alter the bioavailability of radiopharmaceuticals (radiobiocomplexes) labeled with technetium-99 m(99mTc). There are evidences that the bioavailability or the pharmacokinetic of radiobiocomplexes can be modified by some factors, as drugs, due to their toxicological action in specific organs. Vincristine is anatural product that has been utilized in oncology. The vincristine effect on the bioavailability of the radiobiocomplexes 99mTc- ethylenediphosphonic acid (99mTc-MDP) and 99mTc-pyrophosphate (99mTc- PYP) in Balb/c female mice was evaluated. The fragments of kidney were processed to light microscopy and transmission electron microscopy. The aim of this work was to study at structural and ultrastructural levels the alterations caused by vincristine in organs. One hour after the last dose ofvincristine, 99mTc-PYP or 99mTc-MDP was injected, the animals were sacrificed and the percentage of radioactivity (%ATI) was determined in the isolated organs. Concerning 99mTc-PYP, the %ATI (i) decreased in spleen, thymus, lymph nodes (inguinal and mesentheric), kidney, lung, liver, pancreas, stomach, heart and brain and (ii) increased in bone and thyroid. Concerning 99mTc-MDP, the %ATI (iii) decreased in spleen, thymus, lymph nodes (inguinal and mesentheric), kidney, liver, pancreas,stomach, heart, brain, bone, ovary and uterus. In conclusion, the toxic effect of vincristine in determined organs could be responsible for the alteration of the uptake of the studied radiobiocomplexes
Impact of Systemic Inflammation and Autoimmune Diseases on apoA-I and HDL Plasma Levels and Functions
The cholesterol of high-density lipoproteins (HDLs) and its major proteic component, apoA-I, have been widely investigated as potential predictors of acute cardiovascular (CV) events. In particular, HDL cholesterol levels were shown to be inversely and independently associated with the risk of acute CV diseases in different patient populations, including autoimmune and chronic inflammatory disorders. Some relevant and direct anti-inflammatory activities of HDL have been also recently identified targeting both immune and vascular cell subsets. These studies recently highlighted the improvement of HDL function (instead of circulating levels) as a promising treatment strategy to reduce inflammation and associated CV risk in several diseases, such as systemic lupus erythematosus and rheumatoid arthritis. In these diseases, anti-inflammatory treatments targeting HDL function might improve both disease activity and CV risk. In this narrative review, we will focus on the pathophysiological relevance of HDL and apoA-I levels/functions in different acute and chronic inflammatory pathophysiological conditions
Ruta graveolens L. toxicity in Vampirolepis nana infected mice
Objective: To determine possible toxic effects of Ruta graveolens
hydroalcoholic extract in gastrointestinal parasitic infection.
Materials and Methods: A total of 100 g plant leaves and seeds were
powdered and extracted with 1500 mL alcohol/water and administered by
gavage to Swiss albino mice infected with Vampirolepis nana.
Anti-parasitic evaluation and toxicity assays were carried out in six
groups of ten animals each. Treatments were scheduled with both the
leaves and the seeds′ extracts at doses of 2.5, 5, and 10 mg per
gram body weight. Toxicity was comparatively analyzed to a vehicle
control group (n = 10) and to a Praziquantel® treated. On the
fifth day, all the individuals were killed by euthanasia and parasite
scores were correlated, giving rise to a relative percentage of
elimination to each treatment. Toxicity was achieved by hematology and
by clinical chemistry determinations. Results: The use of the R.
graveolens hydroalcoholic extract to treat V. nana infected mice
resulted in a mild-to-moderate hepatoxicity associated to a poor
anti-parasitic effect. The major proglottids elimination (E%) was
achieved at the lowest crude extract concentration with a mild
anti-parasitic efficacy from the highest dose; that did not cause a
significant elimination of parasites. A decrease of circulating
polymorphonuclear-neutrophils associated with a normochromic-normocytic
anemia was detected as the extract dose was augmented. The blood
aspartate-aminotransferase and alanine-aminotransferase tended be
slightly augmented with 100 mg R. graveolens extract. Conclusion: R.
graveolens is an unsafe natural anti-parasitic medicine as its active
constituents may be poorly extracted by the popular crude herb
infusion. Although it presented a mild anti-parasitic effect in mice,
symptoms of natural-products-induced-liver-disease confirmed that its
self-medication should be avoided
Lower cranial nerves function after surgical treatment of Fisch Class C and D tympanojugular paragangliomas
Redox status regulation and action of extra- and intravascular defense mechanisms are associated with bean resistance against Fusarium oxysporum f. sp. phaseoli
Extensive deep vein thrombosis as a complication of testicular cancer treated with the BEP protocol (bleomycin, etoposide and cisplatin): case report
Pharmacological Approaches to Minimizing Cardiometabolic Side Effects of Mood Stabilizing Medications
Association Mapping of Yield and Yield-related Traits Under Reproductive Stage Drought Stress in Rice (Oryza sativa L.)
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