A Collaborative Workflow for the Digitization of Unique Materials

This paper examines the experience of one institution, the University of Maryland Libraries, as it made organizational efforts to harness existing workflows and to capture digitization done in the course of responding to patron requests. By examining the way this organization adjusted its existing workflows to put in place more systematic methods for digital capture of unique collections, the authors hope to provide insight into the benefits and pitfalls of one model for scaling up digitization

Brachiaria brizantha cv. marandu.

Características agronômicas. Exigências e respostas a corretivos e fertilizantes. Produção de matéria seca. Valor nutritivo. Estabelecimento. Produção de sementes. Pragas. Desempenho animal.bitstream/item/104682/1/Brachiaria-brizantha.pd

Potencial forrageiro da Brachiaria brizantha cv. Marandu sob diferentes cargas animal e dosificacao com anti-helmitico em solo de cerrado.

bitstream/item/137570/1/PESQ-EM-ANDAMENTO-25.pdfCNPGC

Modelo estocástico para simulação da precipitação pluviométrica diária de uma região.

Desenvolveu-se um modelo estocastico de precipitacao diaria, em linguagem FORTRAN, para a regiao de Cerrados do Brasil. O modelo foi construido usando-se basicamente duas variaveis: (1) a probabilidade de ocorrencia de chuva em cada dia do mes, e (2) a probabilidade de ocorrencia dessa chuva dentro de certa magnitude. Definiu-se a primeira variavel por uma probabilidade de transicao, que considera a serie historica de precipitacao com base no processo de Markov, onde o sistema passa de um estado a outro, em ensaios sucessivos, considerando o que sucedeu no estado anterior. A segunda variavel foi gerada consecutivamente para cada "dia chuvoso", conforme as probabilidades mensais reais de ocorrencia nas classes. Fizeram-se tres diferentes simulacoes para se avaliar o modelo, e os resultados foram comparados aos dados historicos. As medias simuladas de precipitacao anual foram maiores que as da seire historica, sendo os desvios de apenas 0,9 a 3,6%. As medias mensais e seus desvios-padrao (DP) tambem estiveram relativamente proximos das medias da serie historica, embora os DP das simulacoes de dezembro tenham sido subestimados. Outras variaveis usadas para avaliar o ajuste das simulacoes foram: (1) numero medio de dias de chuva por mes (2) frequencia mensal de precipitacoes segundo a magnitude, (3) frequencia mensal de dias consecutivos de chuva, e (4) frequencia mensal de dias consecutivos sem chuva. Todas as comparacoes mostraram bom ajuste aos dados reais.Título em inglês: Astochastic simulation model of daily precipitation for a region

The ribosomal exit tunnel as a target for optimizing protein expression in Escherichia coli

The folding of many cellular proteins occurs co-translationally immediately outside the ribosome exit tunnel, where ribosomal proteins and other associated factors coordinate the synthesis and folding of newly translated polypeptides. Here, we show that the large subunit protein L29, which forms part of the exit tunnel in Escherichia coli, is required for the productive synthesis of an array of structurally diverse recombinant proteins including the green fluorescent protein (GFP) and an intracellular single-chain Fv antibody. Surprisingly, the corresponding mRNA transcript level of these proteins was markedly less abundant in cells lacking L29, suggesting an unexpected regulatory mechanism that links defects in the exit tunnel to the expression of genetic information. To further highlight the importance of L29 in maintaining protein expression, we used mutagenesis and selection to obtain L29 variants that enhanced GFP expression. Overall, our results suggest that the ribosomal exit tunnel proteins may be key targets for optimizing the overproduction of active, structurally complex recombinant proteins in bacterial cells

“It Is Me Who Endures but My Family That Suffers”: Social Isolation as a Consequence of the Household Cost Burden of Buruli Ulcer Free of Charge Hospital Treatment

Despite free of charge biomedical treatment, the cost burden of Buruli ulcer disease (Bu) hospitalisation in Central Cameroon accounts for 25% of households' yearly earnings, surpassing the threshold of 10%, which is generally considered catastrophic for the household economy, and calling into question the sustainability of current Bu programmes. The high non-medical costs and productivity loss for Bu patients and their households make household involvement in the healing process unsustainable. 63% of households cease providing social and financial support for patients as a coping strategy, resulting in the patient's isolation at the hospital. Social isolation itself was cited by in-patients as the principal cause for abandonment of biomedical treatment. These findings demonstrate that further research and investment in Bu are urgently needed to evaluate new intervention strategies that are socially acceptable and appropriate in the local context

6′′‐Thioether Tobramycin Analogues: Towards Selective Targeting of Bacterial Membranes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/91322/1/ange_201200761_sm_miscellaneous_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/91322/2/5750_ftp.pd

Phase Change Material for Thermotherapy of Buruli Ulcer: A Prospective Observational Single Centre Proof-of-Principle Trial

Buruli ulcer is an infection of the subcutaneous tissue leading to chronic necrotizing skin ulcers. The causative pathogen, Mycobacterium ulcerans, grows best at 30°C–33°C and not above 37°C, and this property makes the application of heat a treatment option. We achieved a breakthrough in heat treatment of Buruli ulcer by employing the phase change material sodium acetate trihydrate as a heat application system for thermotherapy, which is widely used in commercial pocket heat pads. It is easy to apply, rechargeable in hot water, non-toxic and non-hazardous to the environment. Six laboratory reconfirmed patients with ulcerative Buruli lesions were included in the proof-of-principle study and treated for four to six weeks. In patients with small ulcers, wounds healed completely without further intervention. Patients with large defects had skin grafting after successful heat treatment. Heat treatment was not associated with marked increases in local inflammation or the development of ectopic lymphoid tissue. One and a half years after completion of treatment, all patients are relapse-free. The reusable phase change material–based heat application device appears perfectly suited for use in remote Buruli ulcer–endemic areas of countries with limited resources and infrastructure