Voltage-temperature charge verification testing of 34 ampere-hour nickel-cadmium cells

This testing was designed to evaluate various voltage-temperature (V-T) charge curves for use in low-Earth-orbit (LEO) applications of nickel-cadmium battery cells. The trends established relating V-T level to utilizable capacity were unexpected. The trends toward lower capacity at higher V-T levels was predominant in this testing. This effect was a function of the V-T level, the temperature, and the cell history. This effect was attributed to changes occurring in the positive plate. The results imply that for some applications, the use of even lower V-T levels may be warranted. The need to limit overcharge, especially in the early phases of missions, is underlined by this test program

Human osteoarthritis synovium contains an alternatively spliced transcript of ADAMTS4

Purpose: The characterization of an alternatively spliced transcript of the ADAMTS4 aggrecanase. Methods: In human OA synovial cell cultures, RT-PCR was performed using oligonucleotide primers designed to amplify across the exon 8/9 region of human ADAMTS4. The PCR products were purified using a QIAquick purification kit (Qiagen) and sequenced using in house facilities. A pCEP4 (Invitrogen) mammalian expression vector containing ADAMTS4 plus a FLAG epitope was mutated using the QuikChange II site directed mutagenesis kit (Stratagene) to contain the ADAMTS4 splice variant plus a FLAG epitope. The recombinant proteins were purified from HEK293 transfected cells using Anti-FLAG M2 affinity gel (Sigma). Polyclonal antibodies were raised against synthetic peptides representing sequences within the C-terminal region of the splice variant of ADAMTS4 and the raised antibodies were characterized using the recombinant splice variant of ADAMTS4. The antibodies were used in immunohistochemical analysis of human osteoarthritic synovium. The proteolysis of aggrecan and other proteoglycans by the recombinant spice variant of ADAMTS4 was investigated. Results: The degradation of aggrecan is mainly mediated by the aggrecanases, of which ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2) are the best known. We here characterize an alternative splice variant of ADAMTS4. RT-PCR performed as described above resulted in the amplification of normal ADAMTS4, and also a smaller product missing 161 base pairs from the 5’ end of exon 9, the result of alternative splicing in which exon 8 joins to a cryptic 3’ splice site within exon 9. The protein produced by this alternative splicing would lack the spacer domain and have a C-terminus lacking any homologies with the normal ADAMTS4 spacer domain. The alternatively spliced transcript of ADAMTS4 was found in cultured OA synovial cells and in freshly digested OA synovium, but not in human brain, cervix or lung, or in normal bovine synovium. The protein synthesized from this alternatively spliced transcript of ADAMTS4 would lose functions dependent on its spacer domain, like substrate and matrix binding, and inhibition through fibronectin. Removal of the spacer domain from ADAMTS4 has been reported to increase its ability to cleave aggrecan at the Glu373-Ala374 bond, and it may well be that the alternatively spliced transcript produces a protein that is secreted in a more active form. HEK293 cells transfected with a pCEP4 vector containing the cDNA sequence of the splice variant of ADAMTS4 produced the corresponding protein in both the pro and active form. This protein could be found in the media, but mostly associated with the cells, as confirmed using antibodies specific for the splice variant that were produced using synthetic peptides. Immunohistochemical analysis of osteoarthritic synovium using these antibodies showed staining of cells within the synovium. Proteins purified by immunoprecipitation by Anti-FLAG M2 affinity gel from transfected and untransfected HEK293 cells were analysed using the ANASpec SensoLyte 520 Aggrecanase I assay kit. The splice variant had aggrecanase activity comparable to a commercially available ADAMTS4. The splice variant cleaved aggrecan at the G1u373-A1a374 site, as assessed by the neoepitope monoclonal antibody BC3, with activity comparable to ADAMTS4. Conclusions: ADAMTS4 is regulated at multiple levels through control of gene expression, mRNA splicing and protein processing, as well as the expression of naturally occurring inhibitors. We here describe the characteristics of the first known splice variant of ADAMTS4. This alternative splice transcript of ADAMTS4 is expressed as a protein in vivo and can be found in the synovium. It can be speculated that the changes in the C-terminal domain of the protein resulting from this alternatively spliced transcript would have changes in its substrate specificity. The protein produced by the alternative spliced transcript of ADAMTS4 has aggrecanase activity, and the release of low levels of this fully active variant of ADAMTS4 might be a factor in the slow process of superficial zone aggrecan loss in osteoarthritis

Collision and symmetry-breaking in the transition to strange nonchaotic attractors

Strange nonchaotic attractors (SNAs) can be created due to the collision of an invariant curve with itself. This novel ``homoclinic'' transition to SNAs occurs in quasiperiodically driven maps which derive from the discrete Schr\"odinger equation for a particle in a quasiperiodic potential. In the classical dynamics, there is a transition from torus attractors to SNAs, which, in the quantum system is manifest as the localization transition. This equivalence provides new insights into a variety of properties of SNAs, including its fractal measure. Further, there is a {\it symmetry breaking} associated with the creation of SNAs which rigorously shows that the Lyapunov exponent is nonpositive. By considering other related driven iterative mappings, we show that these characteristics associated with the the appearance of SNA are robust and occur in a large class of systems.Comment: To be appear in Physical Review Letter

Optical properties of carbon nanofiber photonic crystals

Carbon nanofibers (CNF) are used as components of planar photonic crystals. Square and rectangular lattices and random patterns of vertically aligned CNF were fabricated and their properties studied using ellipsometry. We show that detailed information such as symmetry directions and the band structure of these novel materials can be extracted from considerations of the polarization state in the specular beam. The refractive index of the individual nanofibers was found to be n_CNF = 4.1.Comment: 10 pages, 4 figure

Toward detailed prominence seismology - II. Charting the continuous magnetohydrodynamic spectrum

Starting from accurate MHD flux rope equilibria containing prominence condensations, we initiate a systematic survey of their linear eigenoscillations. To quantify the full spectrum of linear MHD eigenmodes, we require knowledge of all flux-surface localized modes, charting out the continuous parts of the MHD spectrum. We combine analytical and numerical findings for the continuous spectrum for realistic prominence configurations. The equations governing all eigenmodes for translationally symmetric, gravitating equilibria containing an axial shear flow, are analyzed, along with their flux-surface localized limit. The analysis is valid for general 2.5D equilibria, where either density, entropy, or temperature vary from one flux surface to another. We analyze the mode couplings caused by the poloidal variation in the flux rope equilibria, by performing a small gravity parameter expansion. We contrast the analytical results with continuous spectra obtained numerically. For equilibria where the density is a flux function, we show that continuum modes can be overstable, and we present the stability criterion for these convective continuum instabilities. Furthermore, for all equilibria, a four-mode coupling scheme between an Alfvenic mode of poloidal mode number m and three neighboring (m-1, m, m+1) slow modes is identified, occurring in the vicinity of rational flux surfaces. For realistically prominence equilibria, this coupling is shown to play an important role, from weak to stronger gravity parameter g values. The analytic predictions for small g are compared with numerical spectra, and progressive deviations for larger g are identified. The unstable continuum modes could be relevant for short-lived prominence configurations. The gaps created by poloidal mode coupling in the continuous spectrum need further analysis, as they form preferred frequency ranges for global eigenoscillations.Comment: Accepted by Astronmy & Astrophysics, 21 pages, 15 figure

Giant cutaneous horn in an African woman: a case report

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

Fractal Properties of Robust Strange Nonchaotic Attractors in Maps of Two or More Dimensions

We consider the existence of robust strange nonchaotic attractors (SNA's) in a simple class of quasiperiodically forced systems. Rigorous results are presented demonstrating that the resulting attractors are strange in the sense that their box-counting dimension is N+1 while their information dimension is N. We also show how these properties are manifested in numerical experiments.Comment: 9 pages, 14 figure