Molecular diversification and preliminary evaluations of some satsuma selections’ performance under mediterranean conditions

Satsuma (Citrus unshiu Marc.) has been cultivated for a long time in Turkey, and therefore variations for agronomical traits are likely among cultivated satsumas due to bud mutations. The objectives of this study were to determine variations for some selected agronomical traits and genetic markers among 21 new satsumas derived from selections. Fruit yield, fruit quality and molecular diversification of these clones were determined. The clones of 62 Adana, 4/2 Izmir and 11/1 Izmir indicated the highest yield. The fruits obtained from all clones were heavier than the control (Owari Satsuma). Molecular analysis, as assessed with 9 random amplification of polymorphic DNA (RAPD) and 14 simple sequence repeats (SSR) primers, indicated that satsuma clones showed a narrow genetic base suggesting that the observed morphological polymorphism within the group must be associated with  somatic mutations which were not detected by these molecular markers.Key words: Mandarins, clonal selection, citrus

Genetic characterization of heat tolerant tomato (Solanum lycopersicon) genotypes by SRAP and RAPD markers

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Development Center, and a sample of S. pimpinellifolium were genotyped with RAPD and SRAP markers. Eleven SRAP primer combinations were used and 66 bands were scored. The number of bands scored per primer combination ranged from three to 12, with a mean of six alleles per primer combination. All fragments scored for each primer combination were polymorphic. The percentage of polymorphic products ranged from 25 to 80%. The 15 tomato genotypes were screened for RAPD markers using 50 primers in a PCR-based DNA amplification procedure; 46 primers produced clear and good amplification. Ten of these 46 primers amplified monomorphic fragments in the tomato genotypes. A dendrogram was constructed by combining data from the RAPD and SRAP analyses. Similarity ratios of genotypes ranged from 0.87 to 0.99. The dendrogram was divided into two branches; the first main branch included only genotype CL 5915, and the second main branch included all the other genotypes

Genetic characterization of heat tolerant tomato (Solanum lycopersicon) genotypes by SRAP and RAPD markers

WOS: 000285718400001PubMed ID: 21128206We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Development Center, and a sample of S. pimpinellifolium were genotyped with RAPD and SRAP markers. Eleven SRAP primer combinations were used and 66 bands were scored. The number of bands scored per primer combination ranged from three to 12, with a mean of six alleles per primer combination. All fragments scored for each primer combination were polymorphic. The percentage of polymorphic products ranged from 25 to 80%. The 15 tomato genotypes were screened for RAPD markers using 50 primers in a PCR-based DNA amplification procedure; 46 primers produced clear and good amplification. Ten of these 46 primers amplified monomorphic fragments in the tomato genotypes. A dendrogram was constructed by combining data from the RAPD and SRAP analyses. Similarity ratios of genotypes ranged from 0.87 to 0.99. The dendrogram was divided into two branches; the first main branch included only genotype CL 5915, and the second main branch included all the other genotypes

Molecular diversification and preliminary evaluations of some satsuma selections' performance under mediterranean conditions

Satsuma (Citrus unshiu Marc.) has been cultivated for a long time in Turkey, and therefore variations for agronomical traits are likely among cultivated satsumas due to bud mutations. The objectives of this study were to determine variations for some selected agronomical traits and genetic markers among 21 new satsumas derived from selections. Fruit yield, fruit quality and molecular diversification of these clones were determined. The clones of 62 Adana, 4/2 Izmir and 11/1 Izmir indicated the highest yield. The fruits obtained from all clones were heavier than the control (Owari Satsuma). Molecular analysis, as assessed with 9 random amplification of polymorphic DNA (RAPD) and 14 simple sequence repeats (SSR) primers, indicated that satsuma clones showed a narrow genetic base suggesting that the observed morphological polymorphism within the group must be associated with somatic mutations which were not detected by these molecular markers. © 2011 Academic Journals

In vitro plant regeneration of 'Carrizo' citrange and 'Cleopatra' mandarin by organogenesis

In vitro organogenesis of 'Carrizo' citrange (Citrus sinensis L. × Poncirus trifoliate Raf.) and 'Cleopatra' mandarin (Citrus reshni Hort. ex. Tan.) was studied considering three explant types: cotyledon, epicotyl and root explants. The explants were cultured in MS (Murashige and Skoog, 1962) medium supplemented with 0.0, 2.5, 5.0 mg L-1 6-benzyl-aminopurine (BAP) with 0.5 mg L-1 naphthalene acetic acid (NAA). Compared with cotyledon and epicotyl explants, a higher percentage responded and a higher number of shoots per explant were obtained with root explants, regardless of the 2.5 mg L-1 BAP and 0.5 mg L-1 NAA concentration (4.9 shoots/explant) in 'Carrizo' citrange. Cotyledon explants for both genotypes were not responsive in all plant growth regulator (PGR) concentrations and combinations studied. Only callus formation was obtained from root explants of 'Cleopatra' mandarin in all different PGR concentrations and combinations. 0.5 mg L-1 NAA without BAP gave the best results for shoot formation of 'Cleopatra' mandarin

Determination of self-incompatibility status of some clementine (Citrus clementina) genotypes by histological analysis

Clementine mandarins, is a very important fruit group due the production of high quality fruits that can be seedless as consequence to their sexual self-incompatibility. In this study, the self-compatible/incompatible status of 40 clementine mandarins provided by the Cukurova University collection was determined by histological analysis. For this purpose, self-pollinations were performed and pistil samples were taken 3, 5, 7, 9, 11, 13, 15 and 20 days after pollination. All samples were fixed in FPA70 solution and stored at 4°C. Pistil samples were stained with aniline blue and observed by fluorescence microscope following the staining and squashing. All pollen tubes of the genotypes arrested growth in the upper or middle part of style and none reached the base of the style. As a result, all the 40 clementine cultivars tested have been identified as self-incompatible