Seasonal Variability of Forces Controlling Sedimentation in the Sundarbans National Forest, Bangladesh

Southwest Bangladesh, located on the Ganges-Brahmaputra-Meghna delta, is experiencing the impacts of sea level rise (SLR) due to processes at both the local and global scale. In particular, regional alterations of the hydrodynamic network, due to embankment construction, have drastically altered effective SLR, placing millions of inhabitants at risk of prolonged inundation, and threatening the world’s largest continuous mangrove stand, the Sundarbans National Forest (SNF). In order to effectively employ landscape recovery solutions, an understanding of local sediment transport and deposition is critical. This field-based study investigates the sediment dynamics between the mangrove platform and tidal channels of the SNF using data from a variety of instruments and sediment samples collected within a forested sub-basin (∼20 km2) fed by a major tidal channel. We observe profound seasonal variability within the sub-basin, with the wet season exhibiting a deeper and longer inundation of the mangrove platform and greater suspended sediment concentrations (SSC). Further, there exists a trend of decreasing SSC and median grain size from the perimeter of the SNF to the interior, and decreasing SSC from the tidal channel to the platform at both locations. We project seasonal platform sedimentation rates ranging from 0.17 ± 0.16 cm in the dry season to 1.8 ± 0.35 cm in the wet season. Importantly, the annual deposition rate measured at either location is sufficiently rapid to keep pace with observed rates of effective SLR published in other studies (∼1.0–1.7 cm/year). Based on our results, it appears that many controls on sedimentation are both covariant and of similar importance to land aggradation in the SNF. While inundation depth and frequency will likely increase under future SLR scenarios, sediment supply is threatened by India’s proposed River Linking Project, which could decrease the sediment loads of the Ganges and Brahmaputra Rivers by as much as 75 and 25%, respectively. These rivers provide the sediment for the entire delta, and we predict that with decreasing SSC, some regions—particularly interior sediment-depleted regions—may begin to deteriorate and become submerged, including within the SNF

Proton acceleration by irradiation of isolated spheres with an intense laser pulse

We report on experiments irradiating isolated plastic spheres with a peak laser intensity of 2-3 x 10(20) W cm(-2). With a laser focal spot size of 10 mu m full width half maximum (FWHM) the sphere diameter was varied between 520 nm and 19.3 mu m. Maximum proton energies of similar to 25 MeV are achieved for targets matching the focal spot size of 10 mu m in diameter or being slightly smaller. For smaller spheres the kinetic energy distributions of protons become nonmonotonic, indicating a change in the accelerating mechanism from ambipolar expansion towards a regime dominated by effects caused by Coulomb repulsion of ions. The energy conversion efficiency from laser energy to proton kinetic energy is optimized when the target diameter matches the laser focal spot size with efficiencies reaching the percent level. The change of proton acceleration efficiency with target size can be attributed to the reduced cross-sectional overlap of subfocus targets with the laser. Reported experimental observations are in line with 3D3V particle in cell simulations. They make use of well-defined targets and point out pathways for future applications and experiments.DFG via the Cluster of Excellence Munich-Centre for Advanced Photonics (MAP) Transregio SFB TR18NNSA DE-NA0002008Super-MUC pr48meIvo CermakCGC Instruments in design and realization of the Paul trap systemIMPRS-APSLMUexcellent Junior Research FundDAAD|ToIFEEuropean Union's Horizon research and innovation programme 633053Physic

Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40

Tom40 is an integral protein of the mitochondrial outer membrane, which as the central component of the Translocase of the Outer Membrane (TOM) complex forms a channel for protein import. We characterize the diffusion properties of individual Tom40 molecules fused to the photoconvertable fluorescent protein Dendra2 with millisecond temporal resolution. By imaging individual Tom40 molecules in intact isolated yeast mitochondria using photoactivated localization microscopy with sub-diffraction limited spatial precision, we demonstrate that Tom40 movement in the outer mitochondrial membrane is highly dynamic but confined in nature, suggesting anchoring of the TOM complex as a whole

The genetic epidemiology of joint shape and the development of osteoarthritis

Congruent, low-friction relative movement between the articulating elements of a synovial joint is an essential pre-requisite for sustained, efficient, function. Where disorders of joint formation or maintenance exist, mechanical overloading and osteoarthritis (OA) follow. The heritable component of OA accounts for ~ 50% of susceptible risk. Although almost 100 genetic risk loci for OA have now been identified, and the epidemiological relationship between joint development, joint shape and osteoarthritis is well established, we still have only a limited understanding of the contribution that genetic variation makes to joint shape and how this modulates OA risk. In this article, a brief overview of synovial joint development and its genetic regulation is followed by a review of current knowledge on the genetic epidemiology of established joint shape disorders and common shape variation. A summary of current genetic epidemiology of OA is also given, together with current evidence on the genetic overlap between shape variation and OA. Finally, the established genetic risk loci for both joint shape and osteoarthritis are discussed

Accelerating functional gene discovery in osteoarthritis

Osteoarthritis causes debilitating pain and disability, resulting in a considerable socioeconomic burden, yet no drugs are available that prevent disease onset or progression. Here, we develop, validate and use rapid-throughput imaging techniques to identify abnormal joint phenotypes in randomly selected mutant mice generated by the International Knockout Mouse Consortium. We identify 14 genes with functional involvement in osteoarthritis pathogenesis, including the homeobox gene Pitx1, and functionally characterize 6 candidate human osteoarthritis genes in mouse models. We demonstrate sensitivity of the methods by identifying age-related degenerative joint damage in wild-type mice. Finally, we phenotype previously generated mutant mice with an osteoarthritis-associated polymorphism in the Dio2 gene by CRISPR/Cas9 genome editing and demonstrate a protective role in disease onset with public health implications. We hope this expanding resource of mutant mice will accelerate functional gene discovery in osteoarthritis and offer drug discovery opportunities for this common, incapacitating chronic disease

Genetics and not shared environment explains familial resemblance in adult metabolomics data

Metabolites are small molecules involved in cellular metabolism where they act as reaction substrates or products. The term 'metabolomics' refers to the comprehensive study of these molecules. The concentrations of metabolites in biological tissues are under genetic control, but this is limited by environmental factors such as diet. In adult mono- and dizygotic twin pairs, we estimated the contribution of genetic and shared environmental influences on metabolite levels by structural equation modeling and tested whether the familial resemblance for metabolite levels is mainly explained by genetic or by environmental factors that are shared by family members. Metabolites were measured across three platforms: two based on proton nuclear magnetic resonance techniques and one employing mass spectrometry. These three platforms comprised 237 single metabolic traits of several chemical classes. For the three platforms, metabolites were assessed in 1407, 1037 and 1116 twin pairs, respectively. We carried out power calculations to establish what percentage of shared environmental variance could be detected given these sample sizes. Our study did not find evidence for a systematic contribution of shared environment, defined as the influence of growing up together in the same household, on metabolites assessed in adulthood. Significant heritability was observed for nearly all 237 metabolites; significant contribution of the shared environment was limited to 6 metabolites. The top quartile of the heritability distribution was populated by 5 of the 11 investigated chemical classes. In this quartile, metabolites of the class lipoprotein were significantly overrepresented, whereas metabolites of classes glycerophospholipids and glycerolipids were significantly underrepresented.Analytical BioScience

Exploring voltage mediated delamination of suspended 2D materials as a cause of commonly observed breakdown

Two-dimensional (2D) barrier materials such as graphene, boron nitride, and molybdenum disulfide hold great promise for important applications such as DNA sequencing, desalination, and biomolecular sensing. The 2D materials commonly span pores through an insulating membrane, and electrical fields are applied to drive cross-barrier transport of charged solvated species. While the low-voltage transmembrane transport is well-understood and controllable, high-voltage phenomena are uncontrolled and result in the apparent breakdown of the 2D material's critical insulating properties. Here we use suspended graphene over a 50 nm silicon nitride nanopore as a model system and show that delamination of the 2D material occurs at higher voltages and can directly cause a number of the puzzling high-voltage transport observations. We confirm the occurrence of delamination and observe via atomic force microscopy measurement a micron-scale delaminated patch in a system using chemical vapor deposition graphene. Furthermore, we show that the conductivity of the same system is strongly correlated to the area of delamination via coincident current measurements and optical imaging of the delaminated area. Finally, we demonstrate that delamination alone can cause a dramatic breakdown of barrier function through observation of a reversible increase in conductance of samples prepared with pristine defect-free graphene. These findings should have a great impact on the design and interpretation of 2D barrier material for both experiments and applications