Controlling the Influent Load to Wastewater Treatment Plants

The need for control of the influent load to a wastewater treatment plant (WWTP) is becoming more important. One reason for this is that there are a number of things that cannot be achieved with plant-focused control. For instance it is hard to avoid sludge loss as a result of poor settling or reducing a too high influent flow rate by in-plant control actions. It is also difficult to reduce the effects of a toxin in the influent, if the entire influent is to be biologically treated. Optimisation of the various parts of the collection system, with respect to locally defined objectives, may be counter-productive as it may increase the effluent loads when taking the whole system into account. This is typically the case as optimisation of the control of the sewer net with respect to combined sewer overflows (CSOs) leads to an increased flow to the WWTP. Equalization basins are used to control the flow rate or the load in the sewer net as well as at the WWTPs. The focus has recently been shifted from only reducing the amount of CSOs to reduce the effluent load from the sewer and the WWTP. To minimize the total load from the system the methods previously used to optimise the individual sub-systems must be used together and information from various parts of the system should be available system wide. Due to the cost associated with the construction of equalization basins, the current approach is to increase storage volume by constructing and controlling gates in the sewer net. The potential of system wide control is difficult to estimate, which is exemplified by a discussion on some existing implementations. In this thesis an equalization basin is modelled and used with an existing model of a WWTP. This system is operated with some commonly applied control strategies of equalization basins to estimate the result of control during ideal conditions. Without control of the basin, the possible benefit of construction, or providing an equal amount of storage capacity in the sewer net, is evaluated

Cross-Reactive Human IgM-Derived Monoclonal Antibodies that Bind to HIV-1 Envelope Glycoproteins

Elicitation of antibodies with potent and broad neutralizing activity against HIV by immunization remains a challenge. Several monoclonal antibodies (mAbs) isolated from humans with HIV-1 infection exhibit such activity but vaccine immunogens based on structures containing their epitopes have not been successful for their elicitation. All known broadly neutralizing mAbs (bnmAbs) are immunoglobulin (Ig) Gs (IgGs) and highly somatically hypermutated which could impede their elicitation. Ig Ms (IgMs) are on average significantly less divergent from germline antibodies and are relevant for the development of vaccine immunogens but are underexplored compared to IgGs. Here we describe the identification and characterization of several human IgM-derived mAbs against HIV-1 which were selected from a large phage-displayed naive human antibody library constructed from blood, lymph nodes and spleens of 59 healthy donors. These antibodies bound with high affinity to recombinant envelope glycoproteins (gp140s, Envs) of HIV-1 isolates from different clades. They enhanced or did not neutralize infection by some of the HIV-1 primary isolates using CCR5 as a coreceptor but neutralized all CXCR4 isolates tested although weakly. One of these antibodies with relatively low degree of somatic hypermutation was more extensively characterized. It bound to a highly conserved region partially overlapping with the coreceptor binding site and close to but not overlapping with the CD4 binding site. These results suggest the existence of conserved structures that could direct the immune response to non-neutralizing or even enhancing antibodies which may represent a strategy used by the virus to escape neutralizing immune responses. Further studies will show whether such a strategy plays a role in HIV infection of humans, how important that role could be, and what the mechanisms of infection enhancement are. The newly identified mAbs could be used as reagents to further characterize conserved non-neutralizing, weakly neutralizing or enhancing epitopes and modify or remove them from candidate vaccine immunogens

Designed oligomers of cyanovirin-N show enhanced HIV neutralization

Cyanovirin-N (CV-N) is a small, cyanobacterial lectin that neutralizes many enveloped viruses, including human immunodeficiency virus type I (HIV-1). This antiviral activity is attributed to two homologous carbohydrate binding sites that specifically bind high mannose glycosylation present on envelope glycoproteins such as HIV-1 gp120. We created obligate CV-N oligomers to determine whether increasing the number of binding sites has an effect on viral neutralization. A tandem repeat of two CV-N molecules (CVN_2) increased HIV-1 neutralization activity by up to 18-fold compared to wild-type CV-N. In addition, the CVN_2 variants showed extensive cross-clade reactivity and were often more potent than broadly neutralizing anti-HIV antibodies. The improvement in activity and broad cross-strain HIV neutralization exhibited by these molecules holds promise for the future therapeutic utility of these and other engineered CV-N variants

Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin.

Griffithsin (GRFT), Cyanovirin-N (CV-N) and Scytovirin (SVN) are lectins that inhibit HIV-1 infection by binding to multiple mannose-rich glycans on the HIV-1 envelope glycoproteins (Env). Here we show that these lectins neutralize subtype C primary virus isolates in addition to Env-pseudotyped viruses obtained from plasma and cervical vaginal lavages. Among 15 subtype C pseudoviruses, the median IC50 values were 0.4, 1.8 and 20.1 nM for GRFT, CV-N and SVN, respectively, similar to what was found for subtype B and A. Analysis of Env sequences suggested that concomitant lack of glycans at positions 234 and 295 resulted in natural resistance to these compounds, which was confirmed by site-directed mutagenesis. Furthermore, the binding sites for these lectins overlapped that of the 2G12 monoclonal antibody epitope, which is generally absent on subtype C Env. This data support further research on these lectins as potential microbicides in the context of HIV-1 subtype C infection

Arbetstagares hantering av genomträngligheten och rollkonflikten mellan arbetsliv och privatliv

Den här uppsatsens syfte var att undersöka upplevelserna och hanteringen av gränsen och genomträngligheten mellan arbetslivet och privatlivet hos arbetstagare som arbetar under förtroendetid. Åtta kvalitativa intervjuer genomfördes där deltagarna beskrev sina upplevelser kring fenomenen permeabilitet (genomtränglighet) och work-life conflict (rollkonflikten mellan arbetsliv och privatliv) samt hur dessa fenomen hanterades (boundary management) i situationer då arbetslivet trängde in på privatlivet och vice versa. Intervjusvaren analyserades med en teoretisk tolkning. Resultaten visade att permeabilitet i arbetsdomänen (permeability of the family domain to work) upplevdes vara aningen mer förekommande än permeabilitet i den privata domänen (permeability of the work domain to family). Det här fenomenet benämns asymmetrisk permeabilitet, alltså att fler avbrott tillåts i den ena domänen än i den andra. Vidare framkom att den mentala permeabiliteten i form av tankar ansågs vara svårare att begränsa än den fysiska permeabiliteten i form av kontaktförsök via tekniska medel. Liknande resultat framkom rörande work-life conflict, nämligen att tankar från den ena domänen till den andra framstod som mer konfliktframkallande än fysiska kontaktförsök. I syfte att hantera permeabiliteten och undgå work-life conflict användes olika individuella boundary management-metoder. Vilka metoder som valdes var i hög grad person- och situationsbaserat

A benchmark study of controlled emptying of equalization basins

Storm tanks, or equalization basins, have been used for many years in sewer or wastewater treatment systems to reduce the amount of combined sewer overflows. In this paper, a systematic control of an equalization basin is used not only to dampen or reduce the hydraulic load, but also to systematically improve the effluent quality in a nitrogen removal plant. It is demonstrated that the effluent ammonia concentration is the key component. The Benchmark Simulation Model 1, implemented in MATLAB/Simulink, has been used to perform the evaluations. It has been extended with systematic rainfall generations. Intuitively, it is apparent that the equalization basin should be emptied before a rainfall. This requires a prediction capacity. It is shown that the choice of prediction time is by no means trivial and it is not true that a long prediction horizon is a,ways advantageous