574 research outputs found

    Annual report on U.S. wind power installation, cost, and performance trends: 2006

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    This report--the first in what is envisioned to be an ongoing annual series--attempts to fill this need by providing a detailed overview of developments and trends in the U.S. wind power market, with a particular focus on 2006

    Effects of temperature and pH on equine skeletal muscle mitochondrial membrane potential

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    Despite its significant contribution to metabolism, the relationship between membrane potential ([Delta][Psi]mt) and the physiological extremes of hyperthermia and acidosis has not been investigated. This study uses high-resolution respirometry (HRR) to test the hypothesis that conditions of hyperthermia and acidosis will result in decreased mitochondrial membrane potential.Mitochondria were isolated from fresh semitendinosus muscle biopsies acquired from five Thoroughbred horses. Isolated mitochondria from these biopsies were analyzed using HRR to monitor oxygen consumption and relative changes in [Delta][Psi]mt (using TMRM). Assays will be conducted at simulated baseline physiological conditions (38 degrees C, pH = 7.0) as well as all other combinations of two temperatures (38 degrees C or 43 degrees C) and two pH levels (6.5 or 7.0). ANOVA two factor with replication, with the horse as repeating variable, was used to analyze the resulting data. P<0.05 was considered significantHyperthermia and acidosis resulted in decreased [Delta][Psi]mt both individually and in combination with each other during non-phosphorylating and phosphorylating respiration, although by different magnitudes. Acidosis decreased oxygen consumption during non-phosphorylating and phosphorylating respiration, but hyperthermia had no effect. Oxygen consumption per unit of [Delta][Psi]mt (JO2/[Delta]TMRM) was significantly increased by hyperthermia in both states, but acidosis only affected maximal phosphorylating respirationThe variations in how hyperthermia and acidosis each affected [Delta][Psi]mt and oxygen consumption suggests that they affect the Electron Transfer System (ETS) in different ways. The oxygen results of this study are in direct contrast to previous studies but can be explained by JO2/[Delta]TMRM results which likely show that acidosis inhibited respiration through complex I while hyperthermia increased membrane permeability. This study also demonstrates that ROS production is likely the result of inefficiencies in the ETS rather than proportionate to respiration. This study allows for the observation of comparative differences in membrane potential, but further calibration assays will be required to assign specific voltage across the inner membrane.Although it is likely that they affect mitochondrial function in different ways and to varying degrees, it almost certainly results in decreased ATP synthesis, contributing to the development of fatigue in exercising horses. Understanding how hyperthermia and acidosis affect [Delta][Psi]mt illustrates how they may contribute to the development of fatigue in exercising horses

    The Error in Trial and Error: Exercises on Phrasal Verbs

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    An analysis of 44 commercially available EFL textbooks found that it is common for textbooks to present learners with exercises on phrasal verbs without first providing relevant input to help them. In these cases, the learners are likely to resort to trial-and-error and are then expected to learn from feedback. We report an experiment conducted with Japanese EFL students (N=140) in which we compare the effectiveness of such a trial-and-error method with a retrieval procedure in which students first study a set of phrasal verbs and then complete an exercise. Scores on both an immediate and a one-week delayed post-test suggest superiority of retrieval over the trial-and-error procedure, where, despite the provision of feedback, 25% of the wrong exercise responses were reproduced in the delayed post-test

    Morally Respectful Listening and its Epistemic Consequences

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    What does it mean to listen to someone respectfully, that is, insofar as they are due recognition respect? This paper addresses that question and gives the following answer: it is to listen in such a way that you are open to being surprised. A specific interpretation of this openness to surprise is then defended

    Notions and subnotions in information structure

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    Three dimensions can be distinguished in a cross-linguistic account of information structure. First, there is the definition of the focus constituent, the part of the linguistic expression which is subject to some focus meaning. Second and third, there are the focus meanings and the array of structural devices that encode them. In a given language, the expression of focus is facilitated as well as constrained by the grammar within which the focus devices operate. The prevalence of focus ambiguity, the structural inability to make focus distinctions, will thus vary across languages, and within a language, across focus meanings
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