Report on the In-house Validation of a DNA Extraction Method from Oilseed rape Grains and Validated Method

In accordance with relevant EU legislation , Pioneer Overseas Corporation provided to the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) a DNA extraction method for oilseed rape grains and the relevant samples (ground oilseed rape grains). In line with its mandate , the EU RL GMFF has conducted an in-house validation of this DNA extraction method. To this end it tested the DNA extraction method on the samples provided and evaluated its performance in terms of DNA yield, integrity and quality. The in-house validation study confirmed that the method meets the method performance requirements as established by the EU-RL GMFF and the ENGL , and that it satisfies the provisions of Annex I-2.C.2 to Regulation (EC) No 641/2004. The method is therefore fit for the purpose of producing rapeseed DNA of suitable quantity and quality for subsequent PCR-based analysis. This report is published at http://gmo-crl.jrc.ec.europa.eu/StatusOfDossiers.htm.JRC.I.3-Molecular Biology and Genomic

Rep-pcr de Staphylococcus aureus isolados a partir de mastite bovina na Argentina

The aim of this study was to assess by rep-PCR the genetic relationship of 52 S. aureus strains isolated from mammary infections collected in four herds located in the central dairy region of Argentina. Results were compared with the in vitro activity of antimicrobial drugs frequently used for treating bovine mastitis. Twelve different antimicrobials patterns were observed. Forty eight percent of the strains were susceptible to all antimicrobials tested. rep-PCR typing could successfully differentiate S. aureus strains of bovine origin. At a first level of similarity (50%), it could be defined 5 clusters namely I to V. Most of the strains (75%) were grouped in cluster I. The results may suggest that genotypes were similar in the different herds. Agreement between antibiotic patterns and rep-profiles was not observed for most isolates. The present report describes the genotypes responsible for the mastitis cases in the central dairy region of Argentina. A better knowledge of infective strains distribution in dairy herds might help in formulating strategies to control of infection. Furthermore, antimicrobial susceptibility of S. aureus should be used as guide to select effective drugs to therapy in intramammary infections.O objetivo do presente estudo foi determinar a relação genética entre rep-PCR de 52 linhagens Staphylococcus aureus isoladas de infecções mamárias em quatro fazendas leiteiras da região leiteira central da Argentina. Os resultados foram comparados com a atividade in vitro dos antimicrobianos freqüentemente utilizados no tratamento da mastite bovina. Foram observados 12 diferentes perfis de antimicrobianos. Do total de linhagens, 45% foram suscetíveis a todos os antibióticos ensaiados. A caracterizacão por rep-PCR pode diferenciar com sucesso as linhagens de S. aureus de origem bovina. Num primeiro nível de similaridade (50%) foram definidos cinco grupos denominados de I a V. A maioria das estirpes (75%) agruparam-se no grupo I. Os resultados sugerem que os genotipos foram similares. Os genotipos não foram asociados com os perfis de antimicrobianos na maioria dos isolados. O presente estudo descreve os genotipos responsáveis pelos casos de mastites na região central da Argentina. O melhor conhecimento da distribução das linhagens infectantes em fazendas leiteras poderia auxiliar na formulacão de estratégias para o controle de infecção. Além disso a suscetibilidade aos antimicrobianos de linhagens de S. aureus deve ser usada para montear a selecão de drogas efetivas para a terapia intramamária

Event-specific Method for the Quantification of Maize DAS-40278-9 by Real-time PCR - Validation Report and Validated Method

In line with its mandate1 the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF), in collaboration with the European Network of GMO Laboratories (ENGL), has validated an event-specific polymerase chain reaction (PCR) method for detecting and quantifying maize event DAS-40278-9 (unique identifier DAS-4Ø278-9). The validation study was conducted according to the EU-RL GMFF validation procedure [http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm] and the internationally accepted guidelines2. In accordance with current EU legislation1, Dow AgroSciences LLC has provided the detection method and the positive and negative control samples (genomic DNA from maize seeds harbouring the DAS-40278-9 event as positive control DNA, genomic DNA from conventional maize seeds as negative control DNA). The EU-RL GMFF prepared the validation samples (calibration samples and blind samples at different GM percentage [DNA/DNA]), organised an international collaborative study and analysed the results. The study confirms that the method meets the method performance requirements as established by the EU-RL GMFF and the ENGL in Annex I-2.C.2 to Regulation (EC) No 641/20041 and it fulfils the analytical requirements of Regulation (EU) No 619/2011. This report is published at http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm.JRC.I.3-Molecular Biology and Genomic

Report on the single-laboratory validation of a PCR-based Detection Method for Identification of Florigene™ IFD-25958-3 GM Carnation -Validation Report and Validated Method

In the context of the application for marketing submitted by Florigene Pty Ltd for a genetically modified carnation line (C/NL/09/01) IFD-25958-3, the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) has carried out a single-laboratory validation to assess the performance of a polymerase chain reaction (PCR)-based detection method for detecting and identifying the carnation GM line IFD-25958-3. This report describes the results of tests carried out by the EU-RL GMFF on control samples provided by the method developer and according to the detection method described by the applicant. The taxon-specific method correctly detects the endogenous gene target in genomic DNA of a conventional carnation line (negative control) and in the genomic DNA of the GM carnation line; the same method can also detect the GM target DNA in IFD-25958-3 GM line (positive control) in the experimental conditions described in this report. The Limit of Detection (LOD) of the method has been estimated to be at least 50 copies for the taxon-specific gene and at least 100 copies for the GM insert, based on haploid genome copy number.JRC.I.3-Molecular Biology and Genomic

Jeux de lettres et apprentissage du fulfulde

The problem of teaching the Cameroonian languages has always sharpened the scientific and academic research. The implementation of this teaching is perceived as a practice in which it is necessary to pay attention given that the French and English languages are taking care of the truly authentic national identity of Cameroon. Moreover we observe a lack of political will to redefine the balanced relationship among Cameroonian and official languages. The present reflection proposes to set up a teaching method based on the Fulani word games. It appears as a real boost to the development of faculties or linguistic abilities of the Fulani language learners or not. Hence the question: how can we learn Fulfulde by the usage of its word games and combining business with pleasure? This fundamental question is to instill in the student the skills of graphic and phonetic words of the targeted language through fun. It is about getting learners to learn Fulani by playing word games, games which show the originality of this work for the development, promotion or learning of the local languages through a game that has never been explored, there is therefore a blank field. Guided by an experimental approach, the present contribution is based on existing literature and field surveys, setting the learning of Fulani through a game.

Narrativas da transição : a produção agroecológica de arroz na região metropolitana de Porto Alegre

A região metropolitana de Porto Alegre (RMPA) constitui um dos mais expressivos aglomerados urbanos do Brasil, englobando 34 municípios e cerca de 4 milhões de pessoas. Apesar da urbanização acelerada, a região conta com diversos municípios com expressiva produção agrícola, além da presença significativa de dezessete assentamentos da reforma agrária, os quais somam 1322 famílias. A produção de arroz é destaque, principalmente nos assentamentos de Eldorado do Sul, Nova Santa Rita, Guaíba, Charqueadas e Viamão, onde a opção por sistemas de produção sustentável levou à adoção de uma produção de base ecológica, ou mais especificamente a produção orgânica, que já concentra 501 famílias e 5.513 hectares. As narrativas encontradas nos assentamentos que, em determinado momento, optaram por alterar a sua matriz produtiva para estilos de desenvolvimento rural alinhados com a agroecologia e métodos sustentáveis de produção, fortalecem um contexto mais amplo de transição. Sendo assim, o presente trabalho se propõe a socializar algumas das experiências vivenciadas pelos assentamentos da RMPA, fortalecendo a base científica que dá corpo à agroecologia e ao desenvolvimento rural e auxiliando na difusão de modelos alternativos de produção.The metropolitan region of Porto Alegre (RMPA) is one of the most expressive urban agglomerations in Brazil, concentrating 34 municipalities and around 4 million inhabitants. Despite the accelerated urbanization process, the region has several municipalities with considerable agricultural production, counting with the significant presence of seventeen agrarian reform settlements, which are home to 1322 families. The rice production is a highlight; especially over the settlements of Eldorado do Sul, Nova Santa Rita, Guaíba, Charqueadas and Viamão, where the option for a sustainable production system has led to the adoption of an ecologically based production, or more specifically, an organic production, which already concentrates 501 families and 5.513 hectares. The narratives found in the settlements that, at a given moment, have chosen to change their productive matrix to rural development styles, aligned with agroecology and sustainable production methods, strengthen a broader transition context. Thus, the present work aimed to socialize the RMPA settlements narratives, strengthening the scientific basis of agroecology and rural development studies and helping in the diffusion of alternative models of production

Event-specific Method for the Quantification of Soybean Line A5547-127 Using Real-time PCR

The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the A5547-127 transformation event in soybean DNA (unique identifier ACS-GMØØ6-4). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 ¿on genetically modified food and feed¿ and with Regulation (EC) No 641/2004 of 6 April 2004 ¿on detailed rules for the implementation of Regulation (EC) No 1829/2003¿, Bayer CropScience provided the detection method and the samples (genomic DNA from leaves of plants harbouring the A5547-127 event and from leaves of conventional A5547 soybean plants). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from eight European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is, therefore, considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004. The results of the collaborative study are made publicly available at http://gmo-crl.jrc.it/.JRC.DDG.I.4-Molecular biology and genomic

Narrativas da transição: a produção agroecológica de arroz na região metropolitana de Porto Alegre

A Região Metropolitana de Porto Alegre (RMPA) constitui um dos mais expressivos aglomerados urbanos do Brasil, englobando 34 municípios e cerca de 4 milhões de pessoas. Apesar da urbanização acelerada, a região conta com diversos municípios com expressiva produção agrícola, além da presença significativa de dezessete assentamentos da reforma agrária, os quais somam 1322 famílias. A produção de arroz é destaque, principalmente nos assentamentos de Eldorado do Sul, Nova Santa Rita, Guaíba, Charqueadas e Viamão, onde a opção por sistemas de produção sustentável levou à adoção de uma produção de base ecológica, ou mais especificamente a produção orgânica, que já concentra 501 famílias e 5.513 hectares. As narrativas encontradas nos assentamentos que, em determinado momento, optaram por alterar a sua matriz produtiva para estilos de desenvolvimento rural alinhados com a agroecologia e métodos sustentáveis de produção, fortalecem um contexto mais amplo de transição. Sendo assim, o presente trabalho se propõe a socializar algumas das experiências vivenciadas pelos assentamentos da RMPA, fortalecendo a base científica que dá corpo à agroecologia e ao desenvolvimento rural e auxiliando na difusão de modelos alternativos de produção