Isolasi Dan Karakterisasi Bakteri Endofit Penghasil Inhibitor α-Glukosidase Dari Tanaman Pare (Momordica Charantia L)

Diabetes merupakan penyakit metabolik yang serius di Indonesia. Jumlah penderita diabetes mengalami peningkatan setiap tahunnya. Inhibitor α-glukosidase merupakan senyawa yang dapat menghambat enzim α-glukosidase sehingga bermanfaat sebagai obat antidiabetes. Tanaman Pare (Momordica charantia) diketahui memiliki kasiat sebagai anti diabetes. Senyawa aktif yang dihasilkan tanaman tersebut dapat berasal dari endofit yang hidup di dalam jaringan tanaman. Eksplorasi bakteri endofit dari tanaman pare merupakan salah satu cara untuk mendapatkan isolat bakteri penghasil inhibitor enzim α-glukosidase. Isolasi bakteri dilakukan dengan menginokulasikan sampel tanaman yang telah disterilisasi permukaan pada media agar. Isolat bakteri yang didapat selanjutnya dimurnikan dan di karakterisasi lebih lanjut baik morfologi maupun aktivitas inhibitor α-glukosidasenya. Pengujian aktivitas inhibitor α-glukosidase menggunakan metode spektrofotometer dengan nitrofenil-α-D-glukopiranosida sebagai substrat. Hasil penelitian diperoleh 5 isolat bakteri endofit penghasil inhibior α-glukosidase. Penghambatan terbesar ditunjukkan oleh isolat bakteri Ad 1 yaitu sebesar 27,4%

Special Issue: “Pelvic Floor Disorders: State of the Art and Future Perspectives”

This editorial for the Special Issue “Pelvic Floor Disorders: State of the Art and Future Perspectives” aims to draw attention to the broad field of pelvic floor disorders and serves as an invitation for researchers on a global scaleto share their most recent findings with the urogynecologic community [...

A ten-year study of midwife-led care at an Austrian tertiary care center: a retrospective analysis with special consideration of perineal trauma

Background In contrast to other countries, Austria rarely offers alternative models to medical led-care. In an attempt to improve the facilities, a midwife-led care service was incorporated within the Department of Obstetrics and Fetomaternal Medicine. The aim of the present study was to analyze the maternal and neonatal outcomes of this approach. Methods Over a 10-years period, a total of 2123 low-risk women receiving midwife-led care were studied. Among these women, 148 required obstetric referral. Age- and parity matched low-risk women with spontaneous vaginal birth overseen by an obstetrician-led team were used as controls to ensure comparability of data. Results Midwife-led care management demonstrated a significant decrease in interventions, including oxytocin use (p < 0.001), medical pain relief (p < 0.001), and artificial rupture of membranes (ARM) (p < 0.01) as well as fewer episiotomies (p < 0.001), as compared with obstetrician-led care. Moreover, no negative effects on maternal or neonatal outcomes were observed. The mean length of the second stage of labor, rate of perineal laceration and APGAR scores did not differ significantly between the study groups (p > 0.05). Maternal age (p < 0.01), head diameter (p < 0.001), birth weight (p < 0.001) and the absence of midwife-led care (p < 0.05) were independent risk factors for perineal trauma. The overall referral rate was low (7%) and was most commonly caused by pathologic cardiotocography (CTG) and prolonged first- and second-stage of labor. Most referred mothers nevertheless had spontaneous deliveries (77%), and there were low rates of vaginal operative deliveries and cesarean sections (vacuum extraction, 16%; cesarean section, 7%). Conclusions The present study confirmed that midwife-led care confers important benefits and causes no adverse outcomes for mother and child. The favorable obstetrical outcome clearly highlights the importance of the selection of obstetric care, on the basis of previous risk assessment. We therefore fully support the recommendation that midwife-led care be offered to all low-risk women and that mothers should be encouraged to use this option. However, to increase the numbers of midwife-led care deliveries in Austria in the future, it will be necessary to expand this care model and to establish new midwife-led care units within hospital facilities.(VLID)484439

Ki-67 and vascular endothelial growth factor expression in uterine leiomyosarcoma

Objective. Uterine leiomyosarcomas (LMS) are rare female neoplasms of high malignant potential. The importance of different prognostic parameters in these malignancies remains controversial. The aim of the present study was to evaluate the prognostic value of Ki-67 and vascular endothelial growth factor (VEGF) in uterine LMS

Female Pelvic Floor Dysfunction Continues to Negatively Impact Quality-of-Life during the COVID-19 Lockdown

The COVID-19 pandemic led to dramatical changes in elective medical care. We analysed its impact on patients with female pelvic floor dysfunction during the 6 weeks of lockdown in Austria. A cross-sectional study was conducted: All 99 women who presented at the urogynaecologic outpatient clinic of the Medical University of Vienna with pelvic organ prolapse (POP) or urinary incontinence (UI) from December 2019 up to the lockdown in March 2020 were included and contacted. 97% of these women (96 participants) agreed to participate in the survey conducted to asses pelvic floor related quality of life (QoL) through telephone- interrogation. The mean age was 59 ± 14.8 years, the POP group consisted of 42 women while the UI group included 54 women. Most participants (83% of POP and 81% of UI cases) stated that their female pelvic floor dysfunction had remained equally relevant or had become even more significant during the lockdown. Associated symptoms and psychological strain also maintained their relevance during the lockdown (UI: p = 0.229; POP: p = 0.234). Furthermore, 97% of all interviewed women indicated to be strongly willing to continue their treatment. A generalised linear model regression revealed no clinical or demographic risk factors for psychological strain during the lockdown (p > 0.05). Our results demonstrate that women’s QoL remains significantly impaired by their pelvic-floor disorders even during a worldwide crisis such as COVID-19. Therefore, elective disciplines such as urogynaecology urgently require novel and innovative strategies for continued patient care even in times of a lockdown

Prolapse surgery versus vaginal pessary in women with symptomatic pelvic organ prolapse : which factors influence the choice of treatment?

Objective To investigate which specific clinical factors influence patients choice of prolapse treatment. Methods This study includes a total of 510 cases with symptomatic pelvic organ prolapse (POP) of stage II or higher requiring prolapse treatment. Patients were divided into surgery and pessary groups according to their own choice and treatment preference. Primary outcome of interest was to define potential clinical parameters, which contribute to surgical treatment decision. Results A total of 252/510 (49%) women decided for prolapse surgery and 258/510 (51%) cases were treated conservatively with vaginal pessary. Hypertension, COPD as well as polypharmacy were parameters, which were statistically significantly more common in the pessary group compared to the surgically managed cases (p <0.05). On the contrary, women undergoing prolapse surgery were significantly younger and showed more advanced POP-Q (pelvic organ prolapse quantification) stages (p < 0.05). Clinical factors, such as BMI (body mass index), parity, mode of delivery and postmenopausal status, did not differ between the two groups (p > 0.05). Multiple logistic regression analysis revealed that advanced POP-Q stage (p < 0.001) as well as the absence of smoking (p < 0.001) were independent factors associated with surgical treatment decision. Conclusion Women, who favoured prolapse surgery, were younger and in significant better health condition (less hypertension and COPD), but showed a significantly higher POP-Q stage compared to women choosing pessary treatment. Our data indicate that women with higher POP-Q stage and non-smokers tended to decide for prolapse surgery. This information could help in clinical practice to guide patients for the best possible treatment decision and strengthen individual counselling.(VLID)365714

Effectiveness of hormones in postmenopausal pelvic floor dysfunction-International Urogynecological Association research and development-committee opinion

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