P2X₃ Knock-Out Mice Reveal a Major Sensory Role for Urothelially Released ATP

The present study explores the possible involvement of a purinergic mechanism in mechanosensory transduction in the bladder using P2X₃ receptor knock-out (P2X₃ ⁻⁄⁻) and wild-type control (P2X₃ ⁺⁄⁺) mice. Immunohistochemistry revealed abundant nerve fibers in a suburothelial plexus in the mouse bladder that are immunoreactive to anti-P2X₃. P2X₃ -positive staining was completely absent in the subepithelial plexus of the P2X₃ ⁻⁄⁻ mice, whereas staining for calcitonin gene-related peptide and vanilloid receptor 1 receptors remained. Using a novel superfused mouse bladder–pelvic nerve preparation, we detected a release of ATP proportional to the extent of bladder distension in both P2X₃ ⁻⁄⁻ mice, whereas staining for calcitonin gene-related peptide and vanilloid receptor 1 receptors remained. Using a novel superfused mouse bladder–pelvic nerve preparation, we detected a release of ATP proportional to the extent of bladder distension in both P2X₃ ⁺⁄⁺ and P2X₃ ⁻⁄⁻ mice, although P2X₃ ⁻⁄⁻ bladder had an increased capacity compared with that of the P2X₃ ⁺⁄⁺ bladder. The activity of multifiber pelvic nerve afferents increased progressively during gradual bladder distension (at a rate of 0.1 ml/min). However, the bladder afferents from P2X₃ ⁻⁄⁻ mice showed an attenuated response to bladder distension. Mouse bladder afferents of P2X₃ ⁺⁄⁺, but not P2X₃ ⁻⁄⁻, were rapidly activated by intravesical injections of P2X agonists (ATP or α,β-methylene ATP) and subsequently showed an augmented response to bladder distension. By contrast, P2X antagonists [2′,3′-O-(2,4,6-trinitrophenyl)-ATP and pyridoxal 5-phosphate 6-azophenyl-2′,4′-disulfonic acid] and capsaicin attenuated distension-induced discharges in bladder afferents. These data strongly suggest a major sensory role for urothelially released ATP acting via P2X₃ receptors on a subpopulation of pelvic afferent fibers

Experimental results on mass-thickness distribution in spacecraft equipment

A technique is described for evaluating the shielding properties of spacecraft equipment with respect to cosmic radiation. A gamma-ray source is used in conjunction with a scintillation detector to determine mass-thickness distribution both in plane geometry for equipment units, and in spherical geometry for given points within the spacecraft. Equations are presented for calculating mass-thickness distribution functions, and the results are compared with experimental measurements

Multiresolution analysis for Markov Interval Maps

We set up a multiresolution analysis on fractal sets derived from limit sets of Markov Interval Maps. For this we consider the $\mathbb{Z}$-convolution of a non-atomic measure supported on the limit set of such systems and give a thorough investigation of the space of square integrable functions with respect to this measure. We define an abstract multiresolution analysis, prove the existence of mother wavelets, and then apply these abstract results to Markov Interval Maps. Even though, in our setting the corresponding scaling operators are in general not unitary we are able to give a complete description of the multiresolution analysis in terms of multiwavelets.Comment: 31 pages, 4 figure

Evidence of micro-continent entrainment during crustal accretion

Peer reviewedPublisher PD

CRITERIA FOR EVALUATING A SELECTION SCRENE: SOME PROPOSALS

It is possible to describe a realized selection by means of an indicator, f(x): probability for an individual of value x to be selected. Various models for this indicator are proposed, in the univariate case, and on the assumption that individuals are ranked on a linear index of measured variables. Estimators are defined on the basis of these models for rating traits controlled during selection. A numeric example with ewes is given

Dynamics and management of stage-structured fish stocks

With increasing fishing pressures having brought several stocks to the brink of collapse, there is a need for developing efficient harvesting methods that account for factors beyond merely yield or profit. We consider the dynamics and management of a stage-structured fish stock. Our work is based on a consumer-resource model which De Roos et al. (2008) have derived as an approximation of a physiologically-structured counterpart. First, we rigorously prove the existence of steady states in both models, that the models share the same steady states, and that there exists at most one positive steady state. Furthermore, we carry out numerical investigations which suggest that a steady state is globally stable if it is locally stable Second, we consider multi-objective harvesting strategies which account for yield, profit, and the recovery potential of the fish stock. The recovery potential is a measure of how quickly a fish stock can recover from a major disturbance and serves as an indication of the extinction risk associated with a harvesting strategy. Our analysis reveals that a small reduction in yield or profit allows for a disproportional increase in recovery potential. We also show that there exists a harvesting strategy with yield close to the maximum sustainable yield (MSY) and profit close to that associated with the maximum economic yield (MEY). In offering a good compromise between MSY and MEY, we believe that this harvesting strategy is preferable in most instances. Third, we consider the impact of harvesting on population size structure and analytically determine the most and least harmful harvesting strategies. We conclude that the most harmful harvesting strategy consists of harvesting both adults and juveniles, while harvesting only adults is the least harmful strategy. Finally, we find that a high percentage of juvenile biomass indicates elevated extinction risk and might therefore serve as an early-warning signal of impending stock collapse

Low Frequency Tilt Seismology with a Precision Ground Rotation Sensor

We describe measurements of the rotational component of teleseismic surface waves using an inertial high-precision ground-rotation-sensor installed at the LIGO Hanford Observatory (LHO). The sensor has a noise floor of 0.4 nrad$/ \sqrt{\rm Hz}$ at 50 mHz and a translational coupling of less than 1 $\mu$rad/m enabling translation-free measurement of small rotations. We present observations of the rotational motion from Rayleigh waves of six teleseismic events from varied locations and with magnitudes ranging from M6.7 to M7.9. These events were used to estimate phase dispersion curves which shows agreement with a similar analysis done with an array of three STS-2 seismometers also located at LHO

Testing demand responsive shared transport services via agent-based simulations

Demand Responsive Shared Transport DRST services take advantage of Information and Communication Technologies ICT, to provide on demand transport services booking in real time a ride on a shared vehicle. In this paper, an agent-based model ABM is presented to test different the feasibility of different service configurations in a real context. First results show the impact of route choice strategy on the system performance

Counting reducible, powerful, and relatively irreducible multivariate polynomials over finite fields

We present counting methods for some special classes of multivariate polynomials over a finite field, namely the reducible ones, the s-powerful ones (divisible by the s-th power of a nonconstant polynomial), and the relatively irreducible ones (irreducible but reducible over an extension field). One approach employs generating functions, another one uses a combinatorial method. They yield exact formulas and approximations with relative errors that essentially decrease exponentially in the input size.Comment: to appear in SIAM Journal on Discrete Mathematic

Benchmarking computer platforms for lattice QCD applications

We define a benchmark suite for lattice QCD and report on benchmark results from several computer platforms. The platforms considered are apeNEXT, CRAY T3E, Hitachi SR8000, IBM p690, PC-Clusters, and QCDOC.Comment: 3 pages, Lattice03, machines and algorithm