Comparison of alternative single use harvest technologies for large scale harvests of mammalian cell culture processes

In a fully disposable facility where the use of continuous disk-stack centrifuges are not preferred, harvest processes based on conventional depth filtration become more challenging with increasing single-use bioreactor (SUB) size and higher density culture. Here, several alternative single-use harvest technologies were evaluated. A disposable centrifuge and a range of different synthetic depth filters were tested. Results showed significant improvement in filterability and reduction of depth filter area compared to full traditional depth filtration train

An Empirical Approach to Cosmological Galaxy Survey Simulation: Application to SPHEREx Low-Resolution Spectroscopy

Highly accurate models of the galaxy population over cosmological volumes are necessary in order to predict the performance of upcoming cosmological missions. We present a data-driven model of the galaxy population constrained by deep 0.1-8 $\rm \mu m$ imaging and spectroscopic data in the COSMOS survey, with the immediate goal of simulating the spectroscopic redshift performance of the proposed SPHEREx mission. SPHEREx will obtain over the full-sky $R\sim41$ spectrophotometry at moderate spatial resolution ($\sim6"$) over the wavelength range 0.75-4.18 $\rm \mu m$ and $R\sim135$ over the wavelength range 4.18-5 $\rm \mu m$. We show that our simulation accurately reproduces a range of known galaxy properties, encapsulating the full complexity of the galaxy population and enables realistic, full end-to-end simulations to predict mission performance. Finally, we discuss potential applications of the simulation framework to future cosmology missions and give a description of released data products

Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes

In this study the response of biogas-producing microbiomes to a profound feedstock change was investigated. The microbiomes were adapted to the digestion of either 100% sugar beet, maize silage, or of the silages with elevated amounts of total ammonium nitrogen (TAN) by adding ammonium carbonate or animal manure. The feedstock exchange resulted in a short-range decrease or increase in the biogas yields according to the level of chemical feedstock complexity. Fifteen taxa were found in all reactors and can be considered as generalists. Thirteen taxa were detected in the reactors operated with low TAN and six in the reactors with high TAN concentration. Taxa assigned to the phylum Bacteroidetes and to the order Spirochaetales increased with the exchange to sugar beet silage, indicating an affinity to easily degradable compounds. The recorded TAN-sensitive taxa (phylum Cloacimonetes) showed no specific affinity to maize or sugar beet silage. The archaeal community remained unchanged. The reported findings showed a smooth adaptation of the microbial communities, without a profound negative impact on the overall biogas production indicating that the two feedstocks, sugar beet and maize silage, potentially do not contain chemical compounds that are difficult to handle during anaerobic digestion

Mid-infrared Variability from the Spitzer Deep Wide-field Survey

We use the multi-epoch, mid-infrared Spitzer Deep Wide-Field Survey to investigate the variability of objects in 8.1 deg^2 of the NOAO Deep Wide Field Survey Boötes field. We perform a Difference Image Analysis of the four available epochs between 2004 and 2008, focusing on the deeper 3.6 and 4.5 μm bands. Out of 474, 179 analyzed sources, 1.1% meet our standard variability selection criteria that the two light curves are strongly correlated (r > 0.8) and that their joint variance (σ_(12)) exceeds that for all sources with the same magnitude by 2σ. We then examine the mid-IR colors of the variable sources and match them with X-ray sources from the XBoötes survey, radio catalogs, 24 μm selected active galactic nucleus (AGN) candidates, and spectroscopically identified AGNs from the AGN and Galaxy Evolution Survey (AGES). Based on their mid-IR colors, most of the variable sources are AGNs (76%), with smaller contributions from stars (11%), galaxies (6%), and unclassified objects, although most of the stellar, galaxy, and unclassified sources are false positives. For our standard selection criteria, 11%-12% of the mid-IR counterparts to X-ray sources, 24 μm AGN candidates, and spectroscopically identified AGNs show variability. The exact fractions depend on both the search depth and the selection criteria. For example, 12% of the 1131 known z>1 AGNs in the field and 14%-17% of the known AGNs with well-measured fluxes in all four Infrared Array Camera bands meet our standard selection criteria. The mid-IR AGN variability can be well described by a single power-law structure function with an index of γ ≈ 0.5 at both 3.6 and 4.5 μm, and an amplitude of S _0 ≃ 0.1 mag on rest-frame timescales of 2 yr. The variability amplitude is higher for shorter rest-frame wavelengths and lower luminosities

J. C. Nelkenbrecher's allgemeines Taschenbuch der Münz-, Maß- und Gewichtskunde für Banquiers und Kaufleute

Nelkenbrechers Taschenbuch erschien im Jahre 1828 zum vierzehnten Male, und erfreute sich einer so günstigen Aufnahme, daß schon im Laufe des vorigen Jahres zur Veranstaltungen einer neuen Auflage geschritten werden mußte. ..

Identification of Novel Imprinted Differentially Methylated Regions by Global Analysis of Human-Parthenogenetic-Induced Pluripotent Stem Cells

Parental imprinting is an epigenetic phenomenon by which genes are expressed in a monoallelic fashion, according to their parent of origin. DNA methylation is considered the hallmark mechanism regulating parental imprinting. To identify imprinted differentially methylated regions (DMRs), we compared the DNA methylation status between multiple normal and parthenogenetic human pluripotent stem cells (PSCs) by performing reduced representation bisulfite sequencing. Our analysis identified over 20 previously unknown imprinted DMRs in addition to the known DMRs. These include DMRs in loci associated with human disorders, and a class of intergenic DMRs that do not seem to be related to gene expression. Furthermore, the study showed some DMRs to be unstable, liable to differentiation or reprogramming. A comprehensive comparison between mouse and human DMRs identified almost half of the imprinted DMRs to be species specific. Taken together, our data map novel DMRs in the human genome, their evolutionary conservation, and relation to gene expression