The Rizzoli Multiple Osteochondromas Classification revised: describing the phenotype to improve clinical practice

Multiple osteochondromas (MO) is a rare disorder, characterized by benign osteocartilaginous tumors (osteochondromas), arising from the perichondrium of bones. The osteochondromas increase during growth, frequently causing deformities and limitations. Our study aims to analyze the data captured by the Registry of Multiple Osteochondromas, to refine Istituto Ortopedico Rizzoli (IOR) Classification, providing a representative picture of the phenotypic manifestations throughout the lifespan. We conducted a single-institution cross-sectional study. Patients were categorized according to IOR Classification, which identifies three patients' classes on the presence/absence of deformities and/or limitations. The present dataset was compared with our previously published data, to refine the classification. Nine hundred sixty-eight patients were included: 243 children (<10 years), 136 adolescents (10–15 years), and 589 adults. Of the entire population, half patients presented at least one deformity, and one quarter reported at least one limitation. Compared with our previous study, the amount of children was more than doubled and the percentage of mild/moderate cases was notably increased, giving a better disease overview throughout the lifespan and suggesting a different cut-off for dividing Class II in subclasses. We confirmed that MO is characterized by phenotypic heterogeneity, suggesting that an early classification of the disease may offer a useful tool to follow disease pattern and evolution, to support clinical practice, and to propose timely interventions

Secondary peripheral chondrosarcoma arising in solitary osteochondroma: variables influencing prognosis and survival

Background: Secondary peripheral chondrosarcomas arising in solitary osteochondromas is an unusual complication, reported in small series. In this study, we aimed to present our experience with this rare variant of chondrosarcoma and compare results with already published data in order to determine prognostic factors for overall and disease-free survival. Methods: The case study includes retrospective data from patients diagnosed at a single institution from 1943 to 2019. Clinical data were collected reviewing all available medical records from first to last follow-up visits. To exclude the presence of the Multiple Osteochondroma Hereditary Syndrome, few patients, with a suspect of a familial form of the disease, were evaluated for the presence of germline heterozygous variants in EXT1 and EXT2 genes. Results were summarized using descriptive statistics and statistical analysis were performed to reveal associations between variables. Results: Two hundred and fourteen secondary peripheral chondrosarcomas that arose exclusively from solitary osteochondromas diagnosed in a multidisciplinary setting at the IRCCS Istituto Ortopedico Rizzoli were retrospectively identified, 66.4% males and 33.6% females with a median age at diagnosis of 38 years. The local recurrence rate was 17.3%, while the metastases one was 5.1%. Besides age, a high histologic grade is the only factor associated with worse 5-year and 10-year overall survival (log-rank p = 0.0005, HR = 3.74; 95% CI 1.69–8.26). Moreover, high histological grade (HR = 3.75; 95% CI = 1.69–8.34; p = 0.001) and surgical debulking (HR = 3.71; 95% CI = 1.57–8.79; p = 0.003) were associated with a significantly worse disease-free survival. Conclusions: Our study confirm the low-grade behavior of secondary peripheral chondrosarcomas and demonstrate that the best choice of treatment for those arising in solitary osteochondromas is the wide surgical excision, when possible. Location per se is not a factor that affects prognosis, while the accurate histological grade assessment is correlated with the tumor aggressiveness and a long term follow up is necessary for this rare variant of chondrosarcoma

Wellbeing indicators affecting female entrepreneurship in OECD countries

[EN] The objective of this research is to know which wellbeing indicators, such as work-life balance, educational level, income or job security, are related to the rate of female entrepreneurship in 29 OECD countries. In addition, these countries have been classified according to the motivation of the entrepreneur either by necessity or by opportunity. The empiric study is focused on 29 OECD countries covering the different geographic areas (Western Europe, Central and Eastern Europe, Middle East, etc.) Due to the fact that the sample is relatively small, it is essential to use a selective approach when selecting the causal conditions. To this end, fsQCA is the most appropriate methodology for such a small data set. A total of 5 variables have been used: an independent variable (female TEA ratio), and four dependent variables (work life balance, educational level, sustainable household income and job security). Data measuring female TEA ratio have been obtained from Global Entrepreneur Monitor (GEM in Global report, 2015) data base, while data measuring wellbeing dimensions were taken from the Better Life Index (OECD in How¿s life? Measuring wellbeing, 2015. http://www.oecdbetterlifeindex.org). The results of this piece of research show that countries with high sustainable household income together with high level of education achieves high female entrepreneurship ratio with both, a good work-life balance (despite of a high unemployment probability), or a high labour-personal imbalance (in this latter, with a low probability of unemployment).This work has been funded by the R + D project for emerging research groups with reference (GVA) GV/2016/078.Ribes-Giner, G.; Moya Clemente, I.; Cervelló Royo, RE.; Perelló Marín, MR. (2019). Wellbeing indicators affecting female entrepreneurship in OECD countries. Quality & Quantity. 53(2):915-933. https://doi.org/10.1007/s11135-018-0796-4S915933532Ahl, H., Nelson, T.: How policy positions women entrepreneurs: a comparative analysis of state discourse in Sweden and the United States. J. Bus. Ventur. 30(2), 273–291 (2015). https://doi.org/10.1016/j.jbusvent.2014.08.002Andersson, P.: Happiness and health: wellbeing among the self-employed. J. Socio-Econ. 37(1), 213–236 (2008). https://doi.org/10.1016/j.socec.2007.03.003Bardasi, E., Sabarwal, S., Terrell, K.: How do female entrepreneurs perform? Evidence from three developing regions. Small Bus. Econ. 37(4), 417–441 (2011). https://doi.org/10.1007/s11187-011-9374-zBergheim, S., Schneider, S., Giesel, B., Walter, N.: Measures of Wellbeing. There is More to it Than GDP. Deutsche Bank Research, Frankfurt (2006)Berglund, H.: Between cognition and discourse: phenomenology and the study of entrepreneurship. Int. J. Entrep. Behav. Res. 21(3), 472–488 (2015). https://doi.org/10.1108/IJEBR-12-2013-0210Bianchi, M., Parisi, V., Salvatore, R.: Female entrepreneurs: motivations and constraints. An Italian regional study. Int. J. Gend. Entrep. 8(3), 198–220 (2016). https://doi.org/10.1108/IJGE-08-2015-0029Bittencourt Marconatto, D.A., Barin-Cruz, L., Pozzebon, M., Poitras, J.E.: Developing sustainable business models within BOP contexts: mobilizing native capability to cope with government programs. J. Clean. Prod. 129, 735–748 (2016). https://doi.org/10.1016/j.jclepro.2016.03.038Boarini, R., Comola, M., Smith, C., Manchin, R., de Keulenaer, F.: What makes for a better life? The determinants of subjective well-being in OECD countries—evidence from the Gallup World Poll. OECD statistics working papers, 2012(3), 0_1 (2012). https://doi.org/10.1787/5k9b9ltjm937-enBoarini, R., D’Ercole, M.M.: Going beyond GDP: an OECD perspective. Fisc. Stud. 34(3), 289–314 (2013). https://doi.org/10.1111/j.1475-5890.2013.12007.xBradshaw, J., Hoelscher, P., Richardson, D. (eds.): Comparing child well-being in OECD countries: Concepts and methods. Innocenti working paper, IWP-2006-03. UNICEF Innocenti Research Centre, Florence, Italy (2007)Brindley, C.: Barriers to women achieving their entrepreneurial potential. Int. J. Entrep. Behav. Res. Emerald Group Publishing Limited (2005). Retrieved from http://www.emeraldinsight.com/doi/full/10.1108/13552550510590554Burke, A.E., Fitzroy, F.R., Nolan, M.A.: What makes a die-hard entrepreneur? Beyond the “employee or entrepreneur” dichotomy. Small Bus. Econ. 31(2), 93–115 (2008). https://doi.org/10.1007/s11187-007-9086-6Buttner, H.E., Moore, D.P.: Women’s organizational exodus to entrepreneurship: self-reported motivations and correlates with success. J. Small Bus. Manag. 35(1), 34–46 (1997). Retrieved from http://search.ebscohost.comCarter, S.: The rewards of entrepreneurship: exploring the incomes, wealth, and economic wellbeing of entrepreneurial households. Entrep. Theory Pract. 35(1), 39–55 (2011)Castaño, M.-S., Méndez, M.-T., Galindo, M.Á.: The effect of social, cultural, and economic factors on entrepreneurship. J. Bus. Res. 68(7), 1496–1500 (2015). https://doi.org/10.1016/j.jbusres.2015.01.040Castellano, R., Musella, G., Punzo, G.: Structure of the labour market and wage inequality: evidence from European countries. Qual. Quant. 51(5), 2191–2218 (2017)Cervelló-Royo, R., Moya-Clemente, I., Ribes-Giner, G.: Microfinance Institutions (MFIs) in Latin America: Who should finance the entrepreneurial ventures of the less privileged? In: Peris-Ortiz, M., Sahut, J.-M. (eds.) New Challenges in Entrepreneurship and Finance, pp. 235–245. Springer, Cham (2015)Cervelló-Royo, R., Guijarro, F., Martinez-Gomez, V.: Social performance considered within the global performance of Microfinance Institutions: a new approach. Oper. Res. (2017). https://doi.org/10.1007/s12351-017-0360-3Chaaban, J., Irani, A., Khoury, A.: The composite global wellbeing index (CGWBI): a new multi-dimensional measure of human development. Soc. Indic. Res. 129(1), 465–487 (2016). https://doi.org/10.1007/s11205-015-1112-5Chapple, S.: Child wellbeing and sole-parent family structure in the OECD: an analysis. OECD. Social, employment, and migration working papers (82), 0_1 (2009)Christofides, L.N., Polycarpou, A., Vrachimis, K.: Gender wage gaps, “sticky floors” and “glass ceilings” in Europe. Labour Econ. 21, 86–102 (2013). https://doi.org/10.1016/j.labeco.2013.01.003Cumming, D., Hou, W., Lee, E.: Sustainable and ethical entrepreneurship, corporate finance and governance, and institutional reform in China. J. Bus. Ethics 134(4), 505–508 (2016). https://doi.org/10.1007/s10551-014-2381-7Cummins, R.A., Eckersley, R., Pallant, J., Van Vugt, J., Misajon, R.: Developing a national index of subjective wellbeing: the Australian Unity Wellbeing Index. Soc. Indic. Res. 64(2), 159–190 (2003). https://doi.org/10.1023/A:1024704320683De la Cruz Sánchez-Escobedo, M., Díaz-Casero, J.C., Díaz-Aunión, Á.M., Hernández-Mogollón, R.: Gender analysis of entrepreneurial intentions as a function of economic development across three groups of countries. Int. Entrep. Manag. J. 10(4), 747–765 (2014). https://doi.org/10.1007/s11365-014-0314-7Diener, E.: Subjective wellbeing. In: Diener, E. (ed.) The Sicence of Wellbeing, pp. 11–58. Springer, Dordrecht (2009). https://doi.org/10.1007/978-90-481-2350-6_2Dodge, R., Daly, A., Huyton, J., Sanders, L.: The challenge of defining wellbeing. Int. J. Wellbeing 2(3), 222–235 (2012). https://doi.org/10.5502/ijw.v2i3.4Fiss, P.C.: Building better casual theories: a fuzzy set approach to typologies in organization research. Acad. Manag. J. 54(2), 393–420 (2011)Fleche, S., Smith, C., Sorsa, P.: Exploring determinants of subjective wellbeing in OECD countries (2011)Fleche, S., Smith, C., Sorsa, P.: Exploring determinants of subjective wellbeing in OECD countries evidence from the world value survey. Working papers, OECD Statistics (2012). https://doi.org/10.1787/5kg0k6zlcm5k-enFoley, A., Griffith, B.: Education, training and the promotion of high quality entrepreneurs in the Republic of Ireland. In: Scott, M.G., Rosa, P., Klandt, H. (eds.) Educating Entrepreneurs for Wealth Creation. Ashgate, Aldershot (1998)Garikipati, S.: Microcredit and women’s empowerment: through the lens of time-use data from rural India. Dev. Change 43(3), 719–750 (2012). https://doi.org/10.1111/j.1467-7660.2012.01780.xGEM (Global Entrepreneurship Monitor): Global report (2015)Harter, J.K., Schmidt, F.L., Keyes, C.L.M.: Wellbeing in the workplace and its relationship to business outcomes. A review of the Gallup studies. In: Keyes, C.L.M., Haidt, J. (eds.) Flourishing: The Positive Person and the Good Life, pp. 205–224. American Psychologi cal Association, Washington (2003)Henry, C., Foss, L., Ahl, H.: Gender and entrepreneurship research: a review of methodological approaches. Int. Small Bus. J. 34(3), 217–241 (2015). https://doi.org/10.1177/0266242614549779Hessels, J., Van Gelderen, M., Thurik, R.: Entrepreneurial aspirations, motivations, and their drivers”. Small Bus. Econ. 31(3), 323–339 (2008). https://doi.org/10.1007/s11187-008-9134-xIezzi, D.F., Deriu, F.: Women active citizenship and wellbeing: the Italian case. Qual. Quant. 48(2), 845–862 (2014)Jansson, T.: Housing choices and labor income risk. J. Urban Econ. 99, 107–119 (2017). https://doi.org/10.1016/j.jue.2016.12.006Johansen, V., Foss, L.: The effects of entrepreneurship education—does gender matter? Int. J. Entrep. Small Bus. 20(3), 255–271 (2013). https://doi.org/10.1504/IJESB.2013.056889Judge, W.Q., Hu, H.W., Gabrielsson, J., Talaulicar, T., Witt, M.A., Zattoni, A., Kibler, B.: Configurations of capacity for change in entrepreneurial threshold firms: imprinting and strategic choice perspectives. J. Manag. Stud. 52(4), 506–530 (2015). https://doi.org/10.1111/joms.12121Kautonen, T., Kibler, E., Minniti, M.: Late-career entrepreneurship, income and quality of life. J. Bus. Ventur. 32(3), 318–333 (2017). https://doi.org/10.1016/j.jbusvent.2017.02.005Kephart, P., Schumacher, L.: Has the “glass ceiling” cracked? An exploration of women entrepreneurship. J. Leadersh. Organ. Stud. 12(1), 2–15 (2005). https://doi.org/10.1177/107179190501200102Kirkwood, J.: Motivational factors in a push–pull theory of entrepreneurship. Gend. Manag. Int. J. 24(5), 346–364 (2009). https://doi.org/10.1108/17542410910968805Kobeissi, N.: Gender factors and female entrepreneurship: International evidence and policy implications. J. Int. Entrep. 8, 1–35 (2010). Retrieved from https://doi.org/10.1007/s10843-010-0045-yKuckertz, A., Berger, E.S.C., Allmendinger, M.P.: What drives entrepreneurship? A configurational analysis of the determinants of entrepreneurship in innovation-driven economies. Die Betriebswirtschaft/Bus. Adm. Rev. 75(4), 273–288 (2015). https://doi.org/10.1017/cbo9781107415324.004La Placa, V., McNaught, A., Knight, A.: Discourse on wellbeing in research and practice. Int. J. Wellbeing 3, 116–125 (2013). https://doi.org/10.5502/ijw.v3i1.7Langowitz, N., Minniti, M.: The entrepreneurial propensity of women. Entrep. Theory Pract. 31(3), 341–364 (2007). https://doi.org/10.1111/j.1540-6520.2007.00177.xLee, S.S.Y.: Examining policy configurations as conditions for long-term unemployment and non-standard employment in OECD countries using fuzzy-set analysis. Qual. Quant. 47(6), 3521–3536 (2013)Leffler, E., Svedberg, G.: Enterprise learning: a challenge to education? Eur. Educ. Res. J. 4(3), 219–227 (2005)Levie, J., Autio, E.: A theoretical grounding and test of the GEM model. Small Bus. Econ. 31(3), 235–263 (2008). https://doi.org/10.1007/s11187-008-9136-8Maccagnan, A., Wren-Lewis, S., Brown, H., Taylor, T.: Wellbeing and society: towards quantification of the co-benefits of wellbeing. Soc. Indic. Res. 1–27 (2018)Mandl, C., Berger, E.S.C., Kuckertz, A.: Do you plead guilty? Exploring entrepreneurs’ sensemaking-behavior link after business failure. J. Bus. Ventur. Insights 5, 9–13 (2016). https://doi.org/10.1016/j.jbvi.2015.12.002Martin, B.C., McNally, J.J., Kay, M.J.: Examining the formation of human capital in entrepreneurship: a meta-analysis of entrepreneurship education outcomes. J. Bus. Ventur. 28(2), 211–224 (2013). https://doi.org/10.1016/j.jbusvent.2012.03.002McClelland, E., Swail, J., Bell, J., Ibbotson, P.: Following the pathway of female entrepreneurs. Int. J. Entrep. Behav. Res. 11(2), 84–107 (2005). https://doi.org/10.1108/13552550510590527Millán, J.M., Hessels, J., Thurik, R., Aguado, R.: Determinants of job satisfaction: a European comparison of self-employed and paid employees. Small Bus. Econ. 40(3), 651–670 (2013). https://doi.org/10.1007/s11187-011-9380-1Minniti, M., Nardone, C.: Being in someone else’s shoes: the role of gender in nascent entrepreneurship. Small Bus. Econ. 28(2–3), 223–238 (2007). https://doi.org/10.1007/s11187-006-9017-yMishra, V., Nielsen, I., Smyth, R.: How does relative income and variations in short-run wellbeing affect wellbeing in the long run? Empirical evidence from China’s Korean minority. Soc. Indic. Res. 115(1), 67–91 (2014)Murias, P., Martinez, F., de Miguel, C.: An economic wellbeing index for the Spanish provinces: a data envelopment analysis approach. Soc. Indic. Res. 77(3), 395–417 (2006). https://doi.org/10.1007/s11205-005-2613-4Naudé, W., Amorós, J.E., Cisti, O.: Surfeiting, the appetite may sticken: entrepreneurship and happiness. Small Bus. Econ. 42(3), 523–540 (2014)Ng, T.W.H., Feldman, D.C.: Re-examining the relationship between age and voluntary turnover. J. Vocat. Behav. 74(3), 283–294 (2009). https://doi.org/10.1016/j.jvb.2009.01.004O’Connor, A.: A conceptual framework for entrepreneurship education policy: meeting government and economic purposes. J. Bus. Ventur. 28(4), 546–563 (2013). https://doi.org/10.1016/j.jbusvent.2012.07.003Organisation for Economic Co-operation and Development (OECD): OECD guidelines on measuring subjective well-being (2013)Organization for Economic Cooperation and Development: How’s Life? Measuring Wellbeing (2015). http://www.oecdbetterlifeindex.org . Accessed 28 May 2017Orlova, L.V., Sakhabieva, G.A., Vasyaycheva, V.A., Pronina, N.N.: Impact of educational attainment on the development of female entrepreneurship in Kazakhstan. Indian J. Sci. Technol. (2016). https://doi.org/10.17485/ijst/2016/v9i11/89427Osberg, L., Sharpe, A.: An index of economic wellbeing for selected OECD countries. Rev. Income Wealth 5(3), 291–316 (2002). https://doi.org/10.1111/1475-4991.00056Osberg, L., Sharpe, A.: New Estimates of the Index of Economic Wellbeing for Selected OECD Countries, 1980–2007. Centre for the Study of Living Standards, Ottawa (2009)Parasuraman, S., Purohit, Y.S., Godshalk, V.M., Beutell, N.J.: Work and family variables, entrepreneurial career success, and psychological wellbeing. J. Vocat. Behav. 48(3), 275–300 (1996). https://doi.org/10.1006/jvbe.1996.0025Parasuraman, S., & Simmers, C.A.: Type of employment, work–family conflict and well-being: a comparative study. J. Organ. Behav.: Int. J Ind., Occup. Organ. Psychol. Behav. 22(5), 551–568 (2001)Patrick, C., Stephens, H., Weinstein, A.: Where are all the self-employed women? Push and pull factors influencing female labor market decisions. Small Bus. Econ. 46(3), 365–390 (2016). https://doi.org/10.1007/s11187-015-9697-2Poggesi, S., Mari, M., De Vita, L.: What’s new in female entrepreneurship research? Answers from the literature. Int. Entrep. Manag. J. 12(3), 735–764 (2015). https://doi.org/10.1007/s11365-015-0364-5Ragin, C.: The Comparative Method: Moving Beyond Qualitative and Quantitative Strategies. University of California Press, Berkeley (1987)Ragin, C.C.: New directions in comparative research. In: Kohn, M.L. (ed.) Cross-National Research in Sociology, pp. 57–76. Sage, Newbury Park (1989)Ragin, C.C.: Fuzzy-Set Social Science. University of Chicago Press, Chicago (2000)Ragin, Charles C., Sonnett, J.: Between complexity and parsimony: limited diversity, counterfactual cases, and comparative analysis. In: Ragin, Charles C. (ed.) Redesigning Social Inquiry. University of Chicago Press, Chicago (2004)Ragin, C.C.: Redesigning Social Inquiry: Fuzzy Sets and Beyond, vol. 240. University of Chicago Pres, Chicago (2008)Rahman, S.A., Amran, A., Ahmad, N.H., Taghizadeh, S.K.: Enhancing the wellbeing of base of the pyramid entrepreneurs through business success: the role of private organizations. Soc. Indic. Res. 127(1), 195–216 (2016). https://doi.org/10.1007/s11205-015-0951-4Renee Baptiste, N.: Tightening the link between employee wellbeing at work and performance. Manag. Decis. 46(2), 284–309 (2008). https://doi.org/10.1108/00251740810854168Rey-Martí, A., Tur Porcar, A., Mas-Tur, A.: Linking female entrepreneurs’ motivation to business survival. J. Bus. Res. 68(4), 810–814 (2015). https://doi.org/10.1016/j.jbusres.2014.11.033Reynolds, P.D., Bygrave, W.D., Autio, E., Cox, L.W., Hay, M.: Global entrepreneurship monitor, 2002 executive report. London (2002)Ribes-Giner, G., Moya-Clemente, I., Cervelló-Royo, R., Perello-Marin, M.R.: Domestic economic and social conditions empowering female entrepreneurship. J. Bus. Res. 89, 182–189 (2018)Ruth Eikhof, D., Summers, J., Carter, S., Eikhof, D.R., Summers, J., Carter, S.: Women doing their own thing: media representations of female entrepreneurship. Int. J. Entrep. Behav. Res. 19(5), 547–564 (2013). https://doi.org/10.1108/IJEBR-09-2011-0107Sampedro Gallego, R., Camarero Rioja, L.: Female entrepreneurs in rural Spain—the failed subject of development. Rev. Int. Sociol. 65(48), 121–146 (2007)Saridakis, G., Marlow, S., Storey, D.J.: Do different factors explain male and female self-employment rates? J. Bus. Ventur. 29(3), 345–362 (2014). https://doi.org/10.1016/j.jbusvent.2013.04.004Shepherd, D.A., Douglas, E.J., Shanley, M.: New venture survival: ignorance, external shocks, and risk reduction strategies. J. Bus. Ventur. 15(5), 393–410 (2000). https://doi.org/10.1016/S0883-9026(98)00008-1Shepherd, D., Haynie, J.M.: Birds of a feather don’t always flock together: identity management in entrepreneurship. J. Bus. Ventur. 24(4), 316–337 (2009). https://doi.org/10.1016/j.jbusvent.2007.10.005Shinnar, R.S., Hsu, D.K., Powell, B.C.: Self-efficacy, entrepreneurial intentions, and gender: assessing the impact of entrepreneurship education longitudinally. Int. J. Manag. Educ. 12(3), 561–570 (2014). https://doi.org/10.1016/j.ijme.2014.09.005Shir, N.: Entrepreneurial Wellbeing: The Payoff Structure of Business Creation. Stockholm School of Economics, Stockholm (2015)Thiem, A., Dusa, A.: QCA: a package for qualitative comparative analysis. R package version 2.0. R J. 5, 87–97 (2013). Retrieved from https://cran.r-project.org/web/packages/QCA/index.htmlTorri, M.M.C., Martinez, A.: Women’s empowerment and micro-entrepreneurship in India: constructing a new development paradigm? Prog. Dev. Stud. 14(1), 31–48 (2014). https://doi.org/10.1177/1464993413504347Tur-Porcar, A., Mas-Tur, A., Belso, J.A.: Barriers to women entrepreneurship. Different methods, different results? Qual. Quant. 51(5), 2019–2034 (2017)Uy, M.A., Foo, M.Der, Song, Z.: Joint effects of prior start-up experience and coping strategies on entrepreneurs’ psychological wellbeing. J. Bus. Ventur. 28(5), 583–597 (2013). https://doi.org/10.1016/j.jbusvent.2012.04.003Uy, M.A., Sun, S., Foo, M.Der: Affect spin, entrepreneurs’ wellbeing, and venture goal progress: the moderating role of goal orientation. J. Bus. Ventur. 32(4), 443–460 (2017). https://doi.org/10.1016/j.jbusvent.2016.12.001Van der Sluis, J., Van Praag, M., Vijverberg, W.: Education and entrepreneurship selection and performance: a review of empirical literature. J. Econ. Surv. 22(5), 795–841 (2008)Verheul, I., Stel, A.Van, Thurik, R.: Explaining female and male entrepreneurship at the country level. Entrep. Reg. Dev. 18(2), 151–183 (2006). https://doi.org/10.1080/08985620500532053Witbooi, M., Ukpere, W.: Indigenous female entrepreneurship: analytical study on access to finance for women entrepreneurs in South Africa. Afr. J. Bus. Manag. 5(14), 5646–5657 (2011)Woodside, A.G., Bernal, P.M., Coduras, A.: The general theory of culture, entrepreneurship, innovation, and quality-of-life: comparing nurturing versus thwarting enterprise start-ups in BRIC, Denmark, Germany, and the United States. Ind. Mark. Manag. (2015). https://doi.org/10.1016/j.indmarman.2015.11.00

Caracterização da queixa escolar no Centro de Psicologia Aplicada da Universidade Federal de Juiz de Fora

A queixa escolar vem se constituindo, historicamente, como um dos motivos mais frequentes de encaminhamentos de crianças para os serviços-escola de Psicologia, traduzindo, pela sua complexidade, um desafio para a formação e práticas no âmbito da psicologia escolar/educacional. Diante da necessidade de aperfeiçoar o atendimento à clientela e o processo de triagem, o presente estudo objetivou analisar os prontuários referentes à queixa escolar de pacientes atendidos no Centro de Psicologia Aplicada da Universidade Federal de Juiz de Fora, Minas Gerais, Brasil, num total de 1.590 documentos, no período de 1996 a 2009. Os resultados quanto à clientela evidenciaram a prevalência de meninos na faixa entre 9 e 13 anos de idade, com predomínio de encaminhamentos oriundos de escolas de periférias da cidade. Quanto à queixa, constatou-se expressiva coocorrência de problemas comportamentais e de aprendizagem. Os dados permitem uma reflexão crítica acerca da queixa escolar e de alguns fatores contextuais, bem como a necessidade de aperfeiçoamento da ficha de triagem

Effect of nitric oxide on arachidonic acid release from human amnion-like WISH cells.

In order to clarify the possible interactions between nitric oxide (NO) and arachidonic acid (AA) pathways, human amnion-like WISH cells were perifused to measure the effects of the following substances on [(3)H]arachidonic acid release: (1) sodium nitroprusside (SNP), a nitric oxide donor; (2) 1,1,1-trifluoromethyl-6,9,12,15-heicosatetraen-2-one, a cytosolic phospholipase A(2) (cPLA(2)) inhibitor; (3)L -arginine, the substrate of nitric oxide synthase (NOS); (4) 3-(5'-Hydroxymethyl-2'-furyl)-1-benzylindazole and 1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one, activator and inhibitor of soluble guanylyl cyclase, respectively; (5) a membrane-permeable non-hydrolyzable analogue of guanosine-3',5'-cyclic monophosphate (cGMP). Furthermore, the effect of SNP on prostaglandin E(2) (PGE(2)) release was tested. Exogenous and endogenous NO, as well as the guanylyl cyclase activator and cGMP analogue, significantly increased [(3)H]arachidonic acid release. Both soluble guanylyl cyclase and PLA(2) inhibitors counteracted SNP response. Exogenous NO increased PGE(2) release, although to a much lesser degree compared with arachidonic acid release. Our results indicate that NO stimulates AA release in WISH cells by activating PLA(2) through a cyclic GMP-dependent mechanism

Micellar-type complexes of taylor-made synthetic block copolymers containing the HIV-1 tat DNA for vaccine application.

A novel class of cationic block copolymers constituted by a neutral hydrophilic poly(ethylene glycol) (PEG) block and a positively charged poly(dimethylamino)ethyl methacrylate block was prepared for delivery of DNA. These block copolymers spontaneously assemble with DNA to give in aqueous medium micellar-like structures. Five of these novel block copolymers (K1-5), differing in the length of both the PEG chain and the linear charge density of the poly(dimethylamino)ethyl methacrylate block, were prepared and analyzed for gene delivery, gene expression and safety. All five block copolymers protected DNA from DNAse I digestion and delivered the DNA into the cell. However, only three of them (K1, K2 and K5) released the DNA at level allowing efficient gene expression into cells. No toxic effects of both the copolymers alone or their DNA complexes were observed in vitro or in mice. In addition, copolymers were scarcely immunogenic. These results indicate that this novel class of cationic block copolymers is safe and possesses the biological characteristics required for DNA delivery, thus, representing promising vehicles for DNA vaccination