Differential properties of functions x↦x2t−1x \mapsto x^{2^t-1} -- extended version

We provide an extensive study of the differential properties of the functions $x\mapsto x^{2^t-1}$ over \F, for $2 \leq t \leq n-1$. We notably show that the differential spectra of these functions are determined by the number of roots of the linear polynomials $x^{2^t}+bx^2+(b+1)x$ where $b$ varies in \F.We prove a strong relationship between the differential spectra of $x\mapsto x^{2^t-1}$ and $x\mapsto x^{2^{s}-1}$ for $s= n-t+1$. As a direct consequence, this result enlightens a connection between the differential properties of the cube function and of the inverse function. We also determine the complete differential spectra of $x \mapsto x^7$ by means of the value of some Kloosterman sums, and of $x \mapsto x^{2^t-1}$ for $t \in \{\lfloor n/2\rfloor, \lceil n/2\rceil+1, n-2\}$

A new milestone for the study of variation in Montréal French: The Hochelaga-Maisonneuve sociolinguistic survey

This article introduces the 2012 Montréal FRAN-HOMA corpus, collected in the Francophone neighborhood of Hochelaga-Maisonneuve, and how it relates to the heritage corpora of Montréal French collected since the 1970s. We discuss the methodological choices made regarding the composition of this corpus including the historical and demo-linguistic information that led to the selection of the Hochelaga-Maisonneuve neighborhood for fieldwork. A presentation of the socially stratified sample and criteria for participant selection is followed by a discussion on data collection and the sociolinguistic interview. After a brief review of the transcription protocol and the advantages of audio-aligned transcription, we provide two illustrations of the revival of research on linguistic variation made possible by this new corpus.Cet article présente le corpus FRAN-HOMA de Montréal 2012 recueilli dans le quartier francophone d’Hochelaga-Maisonneuve, et son rapport avec les corpus patrimoniaux de français montréalais recueillis depuis les années 1970. Nous discutons des choix méthodologiques effectués concernant la composition de ce corpus, y compris les informations historiques et démo-linguistiques qui ont conduit à la sélection d’Hochelaga-Maisonneuve comme site pour le travail de terrain. Une présentation de l’échantillon socialement stratifié et des critères de sélection des participants est suivie d’une discussion sur la collecte des données et l’entretien sociolinguistique. Après un bref exposé sur le protocole de transcription et les avantages de la transcription audio-alignée, nous fournissons deux illustrations de la relance de la recherche sur la variation linguistique rendue possible par ce nouveau corpus

Dynamic and Static Approaches Comparison for Test Suite Reduction in Industry

International audienceAutomatic testing constitutes an important part in everyday development practice. IT companies are creating more and more tests to ensure the good behaviour of their applications and gain in efficiency and quality. But running all these tests consumes developer time (hours). This is especially true for the use of large systems involving, for example, the deployment of a web server, or the communication with a database. For this reason tests are not launched as often as they should. Reducing this testing time is a main concern for developers in order to get quick feedback after a change. An interesting solution is to reduce the number of tests to run by identifying those exercising the piece of code changed. Two main approaches seem to be distinguished in the literature: the static and the dynamic. The static approach creates a model of the system and explores it to find the links between the changed methods and the tests. The dynamic approach records the invocations of methods during the execution of test scenarios. We experimented these approaches on several industrial, closed source, cases to understand the strengths and weaknesses of each solution

Test Case Selection in Industry: an Analysis of Issues Related to Static Approaches

International audienceAutomatic testing constitutes an important part of everyday development practice. Worldline, a major IT company, is creating more and more tests to ensure the good behaviour of its applications and gain in efficiency and quality. But running all these tests may take hours. This is especially true for large systems involving, for example, the deployment of a web server or the communication with a database. For this reason tests are not launched as often as they should and are mostly run at night. The company wishes to improve its development and testing process by giving to developers rapid feedback after a change. An interesting solution is to reduce the number of tests to run by identifying only those exercising the piece of code changed. Two main approaches are proposed in the literature: static and dynamic. The static approach creates a model of the source code and explores it to find links between changed methods and tests. The dynamic approach records invocations of methods during the execution of test scenarios. Before deploying a test case selection solution, Worldline created a partnership with us to investigate the situation in its projects and to evaluate these approaches on three industrial, closed source, cases to understand the strengths and weaknesses of each solution. We propose a classification of problems that may arise when trying to identify the tests that cover a method. We give concrete examples of these problems and list some possible solutions. We also evaluate other issues such as the impact on the results of the frequency of modification of methods or considering groups of methods instead of single ones. We found that solutions must be combined to obtain better results, and, problems have different impacts on projects. Considering commits instead of individual methods tends to worsen the results, perhaps due to their large size

What are the Testing Habits of Developers?: A Case Study in a Large IT Company

International audienceTests are considered important to ensure the good behavior of applications and improve their quality. But development in companies also involves tight schedules, old habits, less-trained developers, or practical difficulties such as creating a test database. As a result, good testing practices are not always used as often as one might wish. With a major IT company, we are engaged in a project to understand developers testing behavior, and whether it can be improved. Some ideas are to promote testing by reducing test session length, or by running automatically tests behind the scene and send warnings to developers about the failing ones. Reports on developers testing habits in the literature focus on highly distributed open-source projects, or involve students programmers. As such they might not apply to our industrial, closed source, context. In this paper, we take inspiration from experiments of two papers of the literature to enhance our comprehension of the industrial environment. We report the results of a field study on how often the developers use tests in their daily practice, whether they make use of tests selection and why they do. Results are reinforced by interviews with developers involved in the study. The main findings are that test practice is in better shape than we expected; developers select tests " ruthlessly " (instead of launching an entire test suite); although they are not accurate in their selection, and; contrary to expectation, test selection is not influenced by the size of the test suite nor the duration of the tests

IBD sharing patterns as intra-breed admixture indicators in small ruminants

In this study, we investigated how IBD patterns shared between individuals of the same breed could be informative of its admixture level, with the underlying assumption that the most admixed breeds, i.e. the least genetically isolated, should have a much more fragmented genome. We considered 111 goat breeds (i.e. 2501 individuals) and 156 sheep breeds (i.e. 3304 individuals) from Europe, Africa and Asia, for which beadchip SNP genotypes had been performed. We inferred the breed’s level of admixture from: (i) the proportion of the genome shared by breed’s members (i.e. “genetic integrity level” assessed from ADMIXTURE software analyses), and (ii) the “AV index” (calculated from Reynolds’ genetic distances), used as a proxy for the “genetic distinctiveness”. In both goat and sheep datasets, the statistical analyses (comparison of means, Spearman correlations, LM and GAM models) revealed that the most genetically isolated breeds, also showed IBD profiles made up of more shared IBD segments, which were also longer. These results pave the way for further research that could lead to the development of admixture indicators, based on the characterization of intra-breed shared IBD segments, particularly effective as they would be independent of the knowledge of the whole genetic landscape in which the breeds evolve. Finally, by highlighting the fragmentation experienced by the genomes subjected to crossbreeding carried out over the last few generations, the study reminds us of the need to preserve local breeds and the integrity of their adaptive architectures that have been shaped over the centuries.</p

Reduction of acute mild stress corticosterone response and changes in stress-responsive gene expression in male Balb/c mice after repeated administration of a Rhodiola rosea L. root extract

Rhodiola rosea L. (R. rosea) is an adaptogenic plant increasing body resistance to stress. Its efficacy has been evidenced mainly in chronic stress models, data concerning its effect in acute stress and underlying mechanisms being scarce. The objective was to investigate the effect of repeated doses of a R. rosea hydroethanolic root extract (HRE) on hypothalamic pituitary adrenal response in a murine model of acute mild stress and also the mechanisms involved. Stress response was measured in Balb/c mice having received by gavage HRE (5 g/kg) or vehicle daily for 2 weeks before being submitted to an acute mild stress protocol (open-field test then elevated plus maze). Corticosterone was measured in plasma from mandibular vein blood drawn before and 30, 60, and 90 min after initiation of the stress protocol. Mice were sacrificed at 90 min, and the hippocampus, prefrontal cortex, and amygdala were excised for high-frequency RT-PCR gene expression analysis. At 30 min after acute mild stress induction, corticosterone level in mice having received the HRE was lower than in control mice and comparable to that in nonstressed mice in the HRE group. HRE administration induced brain structure-dependent changes in expression of several stress-responsive genes implicated in neuronal structure, HPA axis activation, and circadian rhythm. In the acute mild stress model used, R. rosea HRE decreased corticosterone level and increased expression of stress-responsive genes, especially in the hippocampus and prefrontal cortex. These findings suggest that R. rosea HRE could be of value for modulating reactivity to acute mild stress

Role of the AP2 β-Appendage Hub in Recruiting Partners for Clathrin-Coated Vesicle Assembly

Adaptor protein complex 2 α and β-appendage domains act as hubs for the assembly of accessory protein networks involved in clathrin-coated vesicle formation. We identify a large repertoire of β-appendage interactors by mass spectrometry. These interact with two distinct ligand interaction sites on the β-appendage (the “top” and “side” sites) that bind motifs distinct from those previously identified on the α-appendage. We solved the structure of the β-appendage with a peptide from the accessory protein Eps15 bound to the side site and with a peptide from the accessory cargo adaptor β-arrestin bound to the top site. We show that accessory proteins can bind simultaneously to multiple appendages, allowing these to cooperate in enhancing ligand avidities that appear to be irreversible in vitro. We now propose that clathrin, which interacts with the β-appendage, achieves ligand displacement in vivo by self-polymerisation as the coated pit matures. This changes the interaction environment from liquid-phase, affinity-driven interactions, to interactions driven by solid-phase stability (“matricity”). Accessory proteins that interact solely with the appendages are thereby displaced to areas of the coated pit where clathrin has not yet polymerised. However, proteins such as β-arrestin (non-visual arrestin) and autosomal recessive hypercholesterolemia protein, which have direct clathrin interactions, will remain in the coated pits with their interacting receptors

AAV-mediated intramuscular delivery of myotubularin corrects the myotubular myopathy phenotype in targeted murine muscle and suggests a function in plasma membrane homeostasis

Myotubular myopathy (XLMTM, OMIM 310400) is a severe congenital muscular disease due to mutations in the myotubularin gene (MTM1) and characterized by the presence of small myofibers with frequent occurrence of central nuclei. Myotubularin is a ubiquitously expressed phosphoinositide phosphatase with a muscle-specific role in man and mouse that is poorly understood. No specific treatment exists to date for patients with myotubular myopathy. We have constructed an adeno-associated virus (AAV) vector expressing myotubularin in order to test its therapeutic potential in a XLMTM mouse model. We show that a single intramuscular injection of this vector in symptomatic Mtm1-deficient mice ameliorates the pathological phenotype in the targeted muscle. Myotubularin replacement in mice largely corrects nuclei and mitochondria positioning in myofibers and leads to a strong increase in muscle volume and recovery of the contractile force. In addition, we used this AAV vector to overexpress myotubularin in wild-type skeletal muscle and get insight into its localization and function. We show that a substantial proportion of myotubularin associates with the sarcolemma and I band, including triads. Myotubularin overexpression in muscle induces the accumulation of packed membrane saccules and presence of vacuoles that contain markers of sarcolemma and T-tubules, suggesting that myotubularin is involved in plasma membrane homeostasis of myofibers. This study provides a proof-of-principle that local delivery of an AAV vector expressing myotubularin can improve the motor capacities of XLMTM muscle and represents a novel approach to study myotubularin function in skeletal muscle