9 research outputs found
Characterization of GFAP expression and cell proliferation in the rat median eminence following hypophysectomy
[EN]To analyze whether the reorganization of the
rat median eminence after hypophysectomy might be
related to changes in glial fibrillary acidic protein
(GFAP)- and cellular proliferation, the distribution of
cells immunoreactive for GFAP and the proliferation rate
of such cells were analyzed at 20, 40 and 60 days posthypophysectomy.
For this study, four rostro-caudal
regions of the median eminence were differentiated: the
retrochiasmatic, preinfundibular, infundibular and
postinfundibular regions. In each of these regions, three
layers were studied: the ependymal, the internal and the
external. At 20 and 40 days after hypophysectomy,
significant increases in cellular proliferation affecting all
three layers studied in the preinfundibular and
infundibular regions were found. At the same time
points, increases in GFAP expression were also
observed. However, after 60 days, GFAP and
proliferative cellular nuclear antigen (PCNA) expression
decreased. Although variations of PCNA and GFAP
levels were evident, no colocalisation of PCNA and
GFAP was found in the cells of the median eminence in
untreated or hypophysectomized rats when sections were
analyzed by double immunohistochemical staining. Our
results suggest that reorganization of median eminence
involves alterations (or modulation) of GFAPimmunoreactive
cells together with a proliferation of
cells that are not GFAP-immunoreactive. This study also
demonstrates that this reorganization is completed within
the first two months after hypophysectomy
Archivo de conferencias y seminarios del Máster en Neurociencias‐INCyL
Memoria ID-188. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014
Ampliación del Simulador para el estudioaprendizaje individualizado en las clases prácticas de Neuroanatomía y Anatomía de los Órganos de los Sentidos. III. Atlas Seccional del Sistema Nervioso Central.
Memoria ID2022-... Ayudas de la Universidad de Salamanca para la innovación docente, curso 2022-2023
Relation among Aromatase P450 and Tumoral Growth in Human Prolactinomas
[EN]The pituitary gland is part of hypothalamic-pituitary–gonadal axis, which controls
development, reproduction, and aging in humans and animals. In addition, the pituitary gland
is regulated mainly by hormones and neurotransmitters released from the hypothalamus and
by systemic hormones secreted by target glands. Aromatase P450, the enzyme responsible for
the catabolization of aromatizable androgens to estrogens, is expressed in different parts of body,
including the pituitary gland. Moreover, aromatase P450 is involved in sexual dimorphism where
alteration in the level of aromatase can initiate a number of diseases in both genders. On the other
hand, the direct actions of estrogens, mainly estradiol, are well known for stimulating prolactin
release. Numerous studies have shown that changes in the levels of estrogens, among other factors,
have been implicated in the genesis and development of prolactinoma. The pituitary gland can
produce estradiol locally in several types of endocrine cells, and it is possible that aromatase could be
responsible for the maintenance of the population of lactotroph cells and the modulation of the action
of central or peripheral regulators. Aromatase overexpression due to inappropriate gene regulation
has clinical effects such as the pathogenesis of prolactinomas. The present study reports on the
synthesis of pituitary aromatase, its regulation by gonadal steroids, and the physiological roles of
aromatase on pituitary endocrine cells. The involvement of aromatase in the pathogenesis of pituitary
tumors, mainly prolactinomas, through the auto-paracrine production of estradiol is reviewed
Desarrollo en Flash de un simulador para el estudio-aprendizaje individualizado en las clases prácticas de la Anatomía Clínica del Tórax
Memoria ID-102. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2019-2020.[Es] Este proyecto tiene como objetivo el desarrollo de una aplicación en flash que permita al estudiante seguir un proceso de aprendizaje durante las prácticas de Anatomía del Tóra
Endothelial immunocytochemical expression of pituitary IL-1β and its relation to ACTH-positive cells is regulated by corticosterone in the male rat
[EN] Interleukin-1 beta (IL-1β) is a cytokine linking the neuroendocrine system and metabolic homeostasis. We have previously demonstrated the relevance of IL-1β for maintaining the pituitary ACTH-producing cells by immuno-blocking its effects in pituitary cultures. However, the morphological characteristics and the intimate relationship of the pituitary cells expressing IL-1β and ACTH remain unknown. For determining pituitary variations of immunoreactivity for IL-1β and its relation with ACTH-positive cells under stress situations, we performed an immunohistochemical analysis of the expression of IL-1β and ACTH in the pituitary gland of adult rats, in the absence or presence of corticosterone, by establishing different groups: untreated, sham-operated, and bilaterally adrenalectomized animals. In the rats subjected to surgery, the glucocorticoid was administered on the same day of the intervention and on the third day post-surgery. Interestingly, it was observed that IL-1β was located in the pituitary endothelial cells at the hypophyseal portal vessels, regardless of the treatment schedule
Sex-related differences in pituitary GH-expressing cells induced by hypothyroidism following treatment with methimazole.
An immunocytochemical and morphometric study of pituitary GH-expressing cells was carried out in adult rats following chronic treatment with methimazole. The morphological results were correlated to the basal GH serum levels. In both sexes, hypothyroidism induced by methimazolee produced a significant decrease of GH serum levels in relation to untreated animals (p<0.05). Morphometrically, methimazole induced significant decreases in the nuclear area (p<0.05) and in the numerical density of immunoreactive cells (p<0.05). In males, these modifications were accompanied by a significant decrease in cellular and cytoplasmic size (p<0.05), but this was not seen in females. Our results confirm the existence of a physiological stimulatory role of thyroid hormones on the activity of pituitary somatotroph cells that must be modulated by gonadal steroids because the effects of hypothyroidism were more evident in male than in female rats
Esplacnología Humana en el EEES: Instrumentos digitales para el aprendizaje práctico y la evaluación continuada. Proyecto piloto para su extensión a otras asignaturas del área de Anatomía.
Memoria ID11-178. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2011-2012
XV International Congress of Control Electronics and Telecommunications: "The role of technology in times of pandemic and post-pandemic: innovation and development for strategic social and productive sectors"
La anterior selección, motivados por la aseveración de Manuel Castells -hace casi 20 años ya- que la innovación y la difusión de la tecnología parecía ser la herramienta apropiada para el desarrollo en la era de la información. Este 2020, sin embargo, ante la situación disruptiva que aquejó y aqueja a la sociedad red como una estructura social emergente de la Era de la Información basada en redes de producción, energizadas por el poder y la experiencia; falló y debe reencontrar su rumbo. Es así que los problemas acuciantes, ahora, fueron: la atención sanitaria y la superación de la epidemia de Sars Cov 2; tomó forma la, hasta entonces, visión irrealista de Castells que … no podemos avanzar con nuestros modelos de desarrollo actual, destruyendo nuestro entorno y excluyendo a la mayor parte de la humanidad de los beneficios de la revolución tecnológica más extraordinaria de la historia, sin sufrir una devastadora reacción por parte de la sociedad y la naturaleza.
Fue así que el Cuarto Mundo, específicamente, donde la suficiencia de recurso humano, de capital, trabajo, información y mercado -vinculados todos a través de la tecnología- supuso que atendería eficazmente a través de la población que podía por su capacidad hacer uso racional y profesional del conocimiento, las necesidades de la mayoritaria población vulnerable y vulnerada.
Por lo anterior, poner en el centro a las personas, en entornos de tarea y trabajo globales hiperconectados combinando espacios físicos, corrientes de información con canales de conexión expeditos, y formando profesionales del conocimiento que asuman y afronten los retos derivados de la transformación digital de empresas, universidades, y organizaciones, pero en condiciones de equidad y sujetos de prosperidad, será el desafío en los escenarios presentes y futuros inmediatos.The previous selection, motivated by the assertion of Manuel Castells -almost 20 years ago- that innovation and diffusion of technology seemed to be the appropriate tool for development in the information age. This 2020, however, in the face of the disruptive situation that afflicted and continues to afflict the network society as an emerging social structure of the Information Age based on production networks, energized by power and experience; He failed and must find his way again. Thus, the pressing problems now were: health care and overcoming the Sars Cov 2 epidemic; Castells' until then unrealistic vision took shape that... we cannot advance with our current development models, destroying our environment and excluding the majority of humanity from the benefits of the most extraordinary technological revolution in history, without suffering a devastating reaction from society and nature.
It was thus that the Fourth World, specifically, where the sufficiency of human resources, capital, work, information and market - all linked through technology - meant that it would serve effectively through the population that could, due to its capacity, make rational use. and knowledge professional, the needs of the majority vulnerable and vulnerable population.
Therefore, putting people at the center, in hyperconnected global task and work environments, combining physical spaces, information flows with expedited connection channels, and training knowledge professionals who assume and face the challenges derived from the digital transformation of companies, universities, and organizations, but in conditions of equality and subject to prosperity, will be the challenge in the present and immediate future scenarios.Bogot