Results on Argentine (Argentina spp.), Bluemouth (Helicolenus dactylopterus), Greater forkbeard (Phycis blennoides) and Spanish ling (Molva macrophthalma) from 2011 Porcupine Bank (NE Atlantic) survey

This paper presents the results on four of the mostimportant deep fish species of the last Porcupine Spanish survey carried in 2011, and updates the document presented in previous years with the information on the first ten years (2001-2010) of the PorcupineSpanish surveys. The document presents total abundances in weight, length frequencies and geographical distributions for Argentina spp. (mostly A. silus, results on A. silus/A. sphiraena distribution in last surveys are provided), bluemouth, greater fork-beard and Spanish ling. Also information on records of Blue ling during the survey series is shown.Versión de edito

Results on Argentine (Argentina spp.), Bluemouth (Helicolenus dactylopterus), Greater forkbeard (Phycis blennoides) and Spanish ling (Molva macrophthalma) from 2010 Porcupine Bank (NE Atlantic) survey

This paper presents the results on four of the mostimportant deep fish species in the Porcupine bottom trawl survey organized by the Spanish Institute of Oceanography in 2010, and updates the documents presented in previous years with the information on the first nine years (2001-2009) of the Porcupine Spanish surveys. The document presents total abundances in weight, length frequencies and geographical distributions for Argentina spp. (mostly A. silus, results on A. silus/A. sphiraena distribution in last survey is presented), bluemouth, greater fork-beard and Spanish ling and information on records of blue ling during the survey series.Versión de edito

Results on Argentine (Argentina spp.), Bluemouth (Helicolenus dactylopterus), Greater forkbeard (Phycis blennoides) and Spanish ling (Molva macrophthalma) from 2008 Porcupine Bank (NE Atlantic) survey

This paper presents the results on four of the most important deep fish species of the last Porcupine Spanish survey carried in 2008, and updates the document presented in the previous year with the information on the first seven years (2001-2007) of the Porcupine Spanish surveys. The document presents total abundances in weight, length frequencies and geographical distributions for Argentina spp. (mostly A. silus), bluemouth, greater fork-beard and Spanish ling.Versión de edito

Results on Argentine (Argentina spp.), Bluemouth (Helicolenus dactylopterus), Greater forkbeard (Phycis blennoides) and Spanish ling (Molva macrophthalma) from 2012 Porcupine Bank (NE Atlantic) survey

This paper presents the results on four of the mostimportant deep fish species of the last Porcupine Spanish survey carried in 2012, and updates the documents presented in previous years with the information on the eleven years (2001-2011) of the Porcupine Spanish bottom trawl surveys on the Porcupine Bank. The document presents total abundances in weight, length frequencies and geographical distributions for Argentina spp. (mostly A. silus, results on proportions by Argentina species distribution in last surveys are provided),bluemouth, greater fork-beard and Spanish ling. All species considered present increases in their abundances, that are especially remarkable in the case of greater forkbeard and Spanish ling, confirming the good recruitments detected in 2011 survey. Besides both species have shown new recruitment peaks in 2012 survey.Versión de edito

Hydrogen sulfide and inflammatory joint diseases

[Abstract] Background: Rheumatoid arthritis (RA) and osteoarthritis (OA) are widespread rheumatic diseases characterized by persistent inflammation and joint destruction. Hydrogen sulfide (H2S) is an endogenous gas with important physiologic functions in the brain, vasculature and other organs. Recent studies have found H2S to be a mediator in inflammatory joint diseases. Objective: This review summarizes the recent literature in this area highlighting relevant developments. Conclusions: Several authors have found that H2S exhibited anti-inflammatory, anti-catabolic and/or anti-oxidant effects in rodent models of acute arthritis and in in vitro models using human synoviocytes and articular chondrocytes from RA and OA tissues. The earliest studies used fast-dissolving salts, such as NaSH, but GYY4137, which produces H2S more physiologically, shortly appeared. More recently still, new H2S-forming compounds that target mitochondria have been synthesized. These compounds open exciting opportunities for investigating the role of H2S in cell bioenergetics, typically altered in arthritides. Positive results have also been obtained when H2S is administered as a sulphurous water bath, an option meriting further study. These findings suggest that exogenous supplementation of H2S may provide a viable therapeutic option for these diseases, particularly in OA

Effect of hydrogen sulfide sources on inflammation and catabolic markers on interleukin 1β-stimulated human articular chondrocytes

[Abstract] Objective. Hydrogen sulfide (H2S), the third gasotransmitter together with NO and CO, is emerging as a regulator of inflammation. To test if it might offer therapeutic value in the treatment of osteoarthritis (OA) we evaluated the effects of two exogenous sources of H2S, NaSH and GYY4137, on inflammation and catabolic markers that characterize OA. Method. Human chondrocytes (CHs) were isolated from OA tissue. Cells were stimulated with a pro-inflammatory cytokine (interleukin-1β, IL1β, 5 ng/ml) and the ability of the two H2S sources to ameliorate its effects on the cells was tested. Nitric oxide (NO) production was quantified through the Griess reaction. Protein levels of inducible NO synthase (NOS2) and matrix metalloproteinase 13 (MMP13) were visualized through immunocytochemistry (ICC). Relative mRNA expression was quantified with qRT-PCR. Prostaglandin-2 (PGE-2), interleukin 6 (IL6) and MMP13 levels were measured with specific EIAs. NFκB nuclear translocation was visualized with immunofluorescence. Results. Both H2S sources led to significant reductions in NO, PGE-2, IL6 and MMP13 released by the cells and at the protein level. This was achieved by downregulation of relevant genes involved in the synthesis routes of these molecules, namely NOS2, cyclooxigenase-2 (COX2), prostaglandin E synthase (PTGES), IL6 and MMP13. NFκB nuclear translocation was also reduced. Conclusion. NaSH and GYY4137 show anti-inflammatory and anti-catabolic properties when added to IL1β activated osteoarthritic CHs. Supplementation with exogenous H2S sources can regulate the expression of relevant genes in OA pathogenesis and progression, counteracting IL1β pro-inflammatory signals that lead to cartilage destruction in part by reducing NFκB activation.Instituto de Salud Carlos III; CIBER BBN CB06/01/004

Synthesis and characterization of sensitive hydrogels based on semi- interpenetrated networks of poly 2-ethyl-(2-pyrrolidone) methacrylate and hyaluronic acid

Sensitive hydrogels attract interest due to their soft wet appearance and shape response to environmental variations. The synthesis and characterization of semi-interpenetrated hydrogels obtained by radical-induced polymerization of 2-ethyl-(2-pyrrolidone)methacrylate (EPM) in the presence of different concentrations of hyaluronic acid (HA) using N,N′-methylene-bisacrylamide or triethylene glycol dimethacrylate as crosslinker, followed by freeze-drying, are described. Polymeric systems were characterized by NMR, FTIR, SEM, TGA, and DMA. PEPMHA hydrogels' mechanical properties and swelling were found to be intimately related to HA concentration and crosslinker. The swelling response was assessed for temperature and pH variation in order to study the behavior of the hydrogels. We found that the presence of HA in PEPM polymeric systems induced a sensitivity to pH variation rather than temperature. Finally, the biocompatibility profile of the hydrogels was evaluated, using mesenchymal stem cells. Cell adhesion and proliferation results revealed the non-cytotoxicity of the systems. We estimate that PEPMHA hydrogels can be used for applications in tissue engineering and for the controlled release of bioactive compounds.Contract grant sponsor: Marie Curie Early Stage Training Alea Jacta Est; contract grant number: MEST-CT-2004-8104The authors thank David Gomez, Sofia Caridade, and Justyna Chojnacka for their technical support and BIOIBERICA for the supply of hyaluronic acid. This work was carried out under the scope of the European NoE EXPERTISSUES, projects MAT 2007-63355, PTDC/QUI/68804/2006 (FCT), CIBER-BBN and Plan-E 2009-0144. In the memory of Prof. Roberto Sastre

Platelet-rich plasma in osteoarthritis treatment: review of current evidence

Review[Abstract] Platelet-rich plasma (PRP) is defined as a volume of plasma with a platelet concentration higher than the average in peripheral blood. Many basic, preclinical and even clinical case studies and trials report PRP’s ability to improve musculoskeletal conditions including osteoarthritis, but paradoxically, just as many conclude it has no effect. The purpose of this narrative review is to discuss the available relevant evidence that supports the clinical use of PRP in osteoarthritis, highlighting those variables we perceive as critical. Here, recent systematic reviews and meta-analyses were used to identify the latest randomized controlled trials (RCTs) testing a PRP product as an intra-articular treatment for knee osteoarthritis, compared with an intra-articular control (mostly hyaluronic acid). Conclusions in the identified RCTs are examined and compared. In total, five recent meta-analyses and systematic reviews were found meeting the above criteria. A total of 19 individual trials were identified in the five reviews but only 9 were level of evidence I RCTs, and many had moderate or high risks of bias. At present, results from these RCTs seem to favor PRP use over other intra-articular treatments to improve pain scales in the short and medium term (6–12 months), but the overall level of evidence is low. As a result, clinical effectiveness of PRP for knee osteoarthritis treatment is still under debate. This is, prominently, the result of a lack of standardization of PRP products, scarceness of high quality RCTs not showing high risks of bias, and poor patient stratification for inclusion in the RCTs.Insituto de Salud Carlos III PI12/00329Insituto de Salud Carlos III; PI16/0212

Impairment of hydrogen sulfide synthesis in chondrocytes under high glucose environment: a link between type 2 diabetes and osteoarthritis

Poster presentatio

Caracterización productiva de las estirpes de cerdo ibérico reconocidas dentro del libro genealógico de la raza y del catálogo oficial de razas de ganado de España

Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente (España