Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture

An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.Facultad de Ciencias Exacta

Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture

An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.Facultad de Ciencias Exacta

Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture

An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS

Lectura de contexto y abordaje psicosocial desde los enfoques narrativos. Bogotá carrera 30, José Acevedo y Gómez.

El presente documento es fruto de la construcción colectiva de conocimiento, la cual inicia con la discusión individual, que mediada por un debate académico obtiene los contenidos y respuestas, enfocadas en el presente documento. Priorizando la necesidad de articular teorías, metodologías y técnicas para canalizar el diagnóstico, acompañamiento y evaluación de situaciones traumáticas, crisis y violencias a las que se ve expuesta una persona, grupo, institución y/o comunidad. Igualmente, se convoca a los estudiantes durante esta fase, a aplicar técnicas de diagnóstico psicosocial en un escenario concreto e identificar subjetividades, e intersubjetividades que emergen en escenarios permeados por la violencia. De igual forma, en aras de cambios o modificar el pensamiento de algunos líderes sociales, se menciona y recuenta en nuestro documento, las magnitudes de daños causados en el ser humano a nivel psicológico y psicosocial, entre otros, del ser humano en su afectación a la humanidad, por las barbaries e inconformidades, sociopolíticas.This document is the result of the collective construction of knowledge, which begins with the individual discussion, which, mediated by an academic debate, obtains the contents and answers, focused on this document. Prioritizing the need to articulate theories, methodologies and techniques to channel the diagnosis, monitoring and evaluation of traumatic situations, crises and violence to which a person, group, institution and / or community is exposed. Likewise, students are summoned during this phase, to apply psychosocial diagnostic techniques in a specific scenario and identify subjectivities, and intersubjectivities that emerge in scenarios permeated by violence. Similarly, for the sake of changes or modify the thinking of some social leaders, it is mentioned and recounted in our document, the magnitude of damage caused to the human being at a psychological and psychosocial level, among others, of the human being in his involvement in humanity, by barbarism and non-conformity, sociopolitical

Procesos de aplicación conceptual y práctico de la normatividad tributaria en contextos investigativos procedimentales tributarios para el fortalecimiento de las competencias disciplinares y profesionales

La presente investigación planteó como objetivo realizar las memorias con las temáticas investigativas que se desarrollaron en el Seminario de Investigación Aplicada, con el fin de actualizar en los participantes y fortalecer sus conocimientos específicos en materia tributaria con base en los temas investigativos dispuestos y orientados por cada docente desde su inicio, elaboración, construcción y presentación ante los docentes evaluadores. Los trabajos cumplen su fin primordial con es fortalecer con los desarrollos temático de cada módulo visto en el SIA sus capacidades y competencias profesionales especialmente en el contexto tributario, en cumplimiento al requerimiento para otorgar al título de Especialistas en Gerencia Tributaria. Luego las memorias compiladas son el resultado de los trabajos presentados y evaluados oportunamente por cada docente comprometido con la calidad en cuanto a las temáticas investigativas, calidad de los contenidos, talleres teóricos prácticos, elementos metodológicos y de más lineamentos institucionales y del programa. La importancia de las memorias radica en su contenido el cual desglosa definiciones, conceptos, desarrollos teóricos prácticos, constituyéndose en un ejemplar de consulta investigativa en áreas de conocimiento fiscal y tributario en el marco de la Ley 1819 de 2016 y sus decretos reglamentarios, en síntesis al interior encontraremos fundamentos teóricos prácticos, procedimentales y resolutivos de casos especiales de Gravamen a los Movimientos Financieros, Monotributo, Renta Personas Naturales, Renta Personas Jurídicas, Procedimiento Tributario, Impuestos Distritales, Normas internacionales de Información Financiera Pymes, entre otros temas