Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection

Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23%, and only AdV C was detected

Inhibition of influenza A virus infection in vitro by peptides designed in silico.

Influenza A viruses are enveloped, segmented negative single-stranded RNA viruses, capable of causing severe human respiratory infections. Currently, only two types of drugs are used to treat influenza A infections, the M2 H(+) ion channel blockers (amantadine and rimantadine) and the neuraminidase inhibitors (NAI) (oseltamivir and zanamivir). Moreover, the emergence of drug-resistant influenza A virus strains has emphasized the need to develop new antiviral agents to complement or replace the existing drugs. Influenza A virus has on the surface a glycoprotein named hemagglutinin (HA) which due to its important role in the initial stage of infection: receptor binding and fusion activities of viral and endosomal membranes, is a potential target for new antiviral drugs. In this work we designed nine peptides using several bioinformatics tools. These peptides were derived from the HA1 and HA2 subunits of influenza A HA with the aim to inhibit influenza A virus infection. The peptides were synthetized and their antiviral activity was tested in vitro against several influenza A viral strains: Puerto Rico/916/34 (H1N1), (H1N1)pdm09, swine (H1N1) and avian (H5N2). We found these peptides were able to inhibit the influenza A viral strains tested, without showing any cytotoxic effect. By docking studies we found evidence that all the peptides were capable to bind to the viral HA, principally to important regions on the viral HA stalk, thus could prevent the HA conformational changes required to carry out its membranes fusion activity

Interaction sites among AVPs derived of C-t of the HA1 subunit targeting influenza A HA (3LZG).

<p>A) Sites of interaction. B) An amplification of C3<b>LB-HA</b> interaction with the proximal domain of HA.</p

Antiviral activity against influenza A H5N2 avian viral strain by AVPs derived from HA1 subunit.

<p>It is expressed as % of cell protection against viral infection, measured by MTT assay. The upper line in each figure indicates the tested AVP.</p

Antiviral activity against influenza A Puerto Rico/916/34 (H1N1) viral strain by AVPs derived from HA2 subunit.

<p>It is expressed as % of cell protection against viral infection, measured by MTT assay. The upper line in each figure indicates the tested AVP.</p

Antiviral activity against influenza A H1N1 swine viral strain by AVPs derived from HA1 subunit.

<p>It is expressed as % of cell protection against viral infection, measured by MTT assay. The upper line in each figure indicates the tested AVP. </p

RESEARCH ARTICLE Body-size structure of Central Iberian mammal fauna reveals semidesertic conditions during the middle Miocene Global Cooling Event

We developed new quantitative palaeoclimatic inference models based on the body-size structure of mammal faunas from the Old World tropics and applied them to the Somosaguas fossil site (middle Miocene, central Iberian Peninsula). Twenty-six mammal species have been described at this site, including proboscideans, ungulates, carnivores, insectivores, lagomorphs and rodents. Our analyses were based on multivariate and bivariate regression models correlating climatic data and body-size structure of 63 modern mammal assemblages from Sub-Saharan Africa and the Indian subcontinent. The results showed an average temperature of the coldest month higher than 26ÊC for the Somosaguas fossil site, a mean annual thermal amplitude around 10ÊC, a drought length of 10 months, and an annual total precipitation greater than 200 mm per year, which are climate conditions typical of an ecotonal zone between the savanna and desert biomes. These results are congruent with the aridity peaks described over the middle Aragonian of Spain and particularly in the local biozone E, which includes Somosaguas. The aridity increase detected in this biozone is associated with the Middle Miocene Global Cooling Event. The environment of Somosaguas around 14 Ma was similar to the current environment in the Sahel region of North Africa, the Horn of Africa, the boundary area between the Kalahari and the Namib in Southern Africa, south-central Arabia, or eastern Pakistan and northwestern India. The distribution of modern vegetation in these regions follows a complex mosaic of plant communities, dominated by scattered xerophilous shrublands, semidesert grasslands, and vegetation linked to seasonal watercourses and ponds.Peer reviewe

Apoyo social y condiciones de vida de adultos mayores que viven en la pobreza urbana en México Social support and living conditions in poor elderly people in urban Mexico

El objetivo del artículo fue analizar el apoyo social y las condiciones de vida de adultos mayores en zonas urbanas empobrecidas. Estudio cualitativo con 8 grupos focales, realizado en Guerrero, Jalisco, Morelos y Sinaloa, México, durante 2005-2006. Participaron 40 varones y 63 mujeres. El principal apoyo para la vida cotidiana y la condición de salud de los adultos mayores proviene de los familiares cercanos, en algunos casos de vecinos. Los apoyos de los adultos mayores son básicamente materiales y económicos, así como cierto tipo de acompañamiento y traslado a consultas médicas. El apoyo emocional es mínimo o inexistente, igual la compañía cotidiana y la integración en la vida familiar. Se identificó una ausencia significativa de apoyo por parte del gobierno o de organizaciones religiosas o civiles. La familia continúa siendo la fuente fundamental de apoyo. Es necesario incrementar las acciones gubernamentales para la población adulta mayor y transformar la idea de que las necesidades de esta población es una responsabilidad individual más que colectiva.<br>The aim of this paper was to analyze social support and living conditions among poor elderly people in Mexican cities. A qualitative study with eight focus groups was carried out in Guadalajara, Cuernavaca, Chilpancingo, and Culiacan, Mexico, in 2005. Forty men and 63 women participated in the study. The main support for the elderly in daily living came from their immediate family and in some cases from neighbors. Social support was basically material and economic, in addition to providing company and transportation for medical appointments. Daily emotional support, companionship, and social inclusion were minimal or absent. The study identified a significant lack of support from government and religious or civil society organizations. The family is still the main source of support for the elderly. Increased government collaboration is dramatically needed to combat the misconception that the needs of the elderly are the individual family's responsibility rather than a collaborative effort by society