Convective instability and transient growth in flow over a backward-facing step

Transient energy growths of two- and three-dimensional optimal linear perturbations to two-dimensional flow in a rectangular backward-facing-step geometry with expansion ratio two are presented. Reynolds numbers based on the step height and peak inflow speed are considered in the range 0–500, which is below the value for the onset of three-dimensional asymptotic instability. As is well known, the flow has a strong local convective instability, and the maximum linear transient energy growth values computed here are of order 80×103 at Re = 500. The critical Reynolds number below which there is no growth over any time interval is determined to be Re = 57.7 in the two-dimensional case. The centroidal location of the energy distribution for maximum transient growth is typically downstream of all the stagnation/reattachment points of the steady base flow. Sub-optimal transient modes are also computed and discussed. A direct study of weakly nonlinear effects demonstrates that nonlinearity is stablizing at Re = 500. The optimal three-dimensional disturbances have spanwise wavelength of order ten step heights. Though they have slightly larger growths than two-dimensional cases, they are broadly similar in character. When the inflow of the full nonlinear system is perturbed with white noise, narrowband random velocity perturbations are observed in the downstream channel at locations corresponding to maximum linear transient growth. The centre frequency of this response matches that computed from the streamwise wavelength and mean advection speed of the predicted optimal disturbance. Linkage between the response of the driven flow and the optimal disturbance is further demonstrated by a partition of response energy into velocity components

Convective instability and transient growth in steady and pulsatile stenotic flows

We show that suitable initial disturbances to steady or long-period pulsatile flows in a straight tube with an axisymmetric 75%-occlusion stenosis can produce very large transient energy growths. The global optimal disturbances to an initially axisymmetric state found by linear analyses are three-dimensional wave packets that produce localized sinuous convective instability in extended shear layers. In pulsatile flow, initial conditions that trigger the largest disturbances are either initiated at, or advect to, the separating shear layer at the stenosis in phase with peak systolic flow. Movies are available with the online version of the paper

Stress corrosion cracking of titanium alloys: Studies of cracks in thin specimens; SCC of Ti-6Al-4V in chloride, iodide and fluoride solutions; stress corrosion cracking in molten salts; electrochemistry of freshly generated titanium surfaces

Electrochemistry of freshly generated titanium surfaces and stress corrosion cracking in aqueous solutions and in molten salt

Unexpected phase locking of magnetic fluctuations in the multi-k magnet USb

The spin waves in the multi-k antiferromagnet USb soften and become quasielastic well below the antiferromagnetic ordering temperature TN. This occurs without a magnetic or structural transition. It has been suggested that this change is in fact due to dephasing of the different multi-k components: a switch from 3-k to 1-k behavior. In this work, we use inelastic neutron scattering with tridirectional polarization analysis to probe the quasielastic magnetic excitations and reveal that the 3-k structure does not dephase. More surprisingly, the paramagnetic correlations also maintain the same clear phase correlations well above TN (up to at least 1.4TN)

Metabolomic profiling and stable isotope labelling of Trichomonas vaginalis and Tritrichomonas foetus reveal major differences in amino acid metabolism including the production of 2-hydroxyisocaproic acid, cystathionine and S-methylcysteine

Trichomonas vaginalis and Tritrichomonas foetus are pathogens that parasitise, respectively, human and bovine urogenital tracts causing disease. Using LC-MS, reference metabolomic profiles were obtained for both species and stable isotope labelling with D-[U-13C6] glucose was used to analyse central carbon metabolism. This facilitated a comparison of the metabolic pathways of T. vaginalis and T. foetus, extending earlier targeted biochemical studies. 43 metabolites, whose identities were confirmed by comparison of their retention times with authentic standards, occurred at more than 3-fold difference in peak intensity between T. vaginalis and T. foetus. 18 metabolites that were removed from or released into the medium during growth also showed more than 3-fold difference between the species. Major differences were observed in cysteine and methionine metabolism in which homocysteine, produced as a bi-product of trans-methylation, is catabolised by methionine γ-lyase in T. vaginalis but converted to cystathionine in T. foetus. Both species synthesise methylthioadenosine by an unusual mechanism, but it is not used as a substrate for methionine recycling. T. vaginalis also produces and exports high levels of S-methylcysteine, whereas only negligible levels were found in T. foetus which maintains significantly higher intracellular levels of cysteine. 13C-labeling confirmed that both cysteine and S-methylcysteine are synthesised by T. vaginalis; S-methylcysteine can be generated by recombinant T. vaginalis cysteine synthase using phosphoserine and methanethiol. T. foetus contained higher levels of ornithine and citrulline than T. vaginalis and exported increased levels of putrescine, suggesting greater flux through the arginine dihydrolase pathway. T. vaginalis produced and exported hydroxy acid derivatives of certain amino acids, particularly 2-hydroxyisocaproic acid derived from leucine, whereas negligible levels of these metabolites occurred in T. foetus

Raman study of the Verwey transition in Magnetite at high-pressure and low-temperature; effect of Al doping

We report high-pressure low-temperature Raman studies of the Verwey transition in pure and Al-doped magnetite (Fe_3O_4). The low temperature phase of magnetite displays a number of additional Raman modes that serve as transition markers. These transition markers allow one to investigate the effect of hydrostatic pressure on the Verwey transition temperature. Al-doped magnetite Fe_2.8Al_0.2O_4 (TV=116.5K) displays a nearly linear decrease of the transition temperature with an increase of pressure yielding dP/dT_V = -0.096 GPa/K. In contrast pure magnetite displays a significantly steeper slope of the PT equilibrium line with dP/dT_V = -0.18 GPa/K. The slope of the PT equilibrium lines is related to the changes of the molar entropy and molar volume at the transition. We compare our spectroscopic data with that obtained from the ambient pressure specific heat measurements and find a good agreement in the optimally doped magnetite. Our data indicates that Al doping leads to a smaller entropy change and larger volume expansion at the transition. Our data displays the trends that are consistent with the mean field model of the transition that assumes charge ordering in magnetite.Comment: 17 pages, 3 figure

A Case Study on Accessible Reading with Deaf Children

The concept of accessible reading for deaf students is new and worthy of exploration. In the face of the reading difficulties often experienced by deaf students, the lack of a specialized reading methodology that works for them must be addressed. Central to the paper is a research case study undertaken with two young deaf students, proficient in American Sign Language (ASL) and learning to read. The students participated in a tutorial with a tutor knowledgeable in a specialized reading methodology called ASL Gloss. The participating students demonstrated progress in reading skills over time. Two reading measures were adapted from English to ASL for use with deaf students. Some important features of ASL Gloss are included in the study report. The manipulated English text that closer resembles ASL and the use of the ASL-phabet, are designed to facilitate deaf students’ needed transition from ASL to English literacy (Supalla, 2017; Supalla & Cripps, 2011; Supalla et al., 2001). These deaf students engage in oral reading (in ASL) and are also given a different task. That is, to identify ASL-phabet letters that represent the phonological structure of signed words. The reading measures under development appear to promote the process of learning to read as informed by the quantitative and qualitative data. These findings support the need and promise of pursuing an alternative theory and applied research for deaf students’ reading that accounts for their ability to become fluent readers

Instability modes and transition of pulsatile stenotic flow: pulse-period dependence

Accepted versio

Visualization of actin filaments and monomers in somatic cell nuclei

© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 24 (2013): 982-994, doi:10.1091/mbc.E12-09-0685.In addition to its long-studied presence in the cytoplasm, actin is also found in the nuclei of eukaryotic cells. The function and form (monomer, filament, or noncanonical oligomer) of nuclear actin are hotly debated, and its localization and dynamics are largely unknown. To determine the distribution of nuclear actin in live somatic cells and evaluate its potential functions, we constructed and validated fluorescent nuclear actin probes. Monomeric actin probes concentrate in nuclear speckles, suggesting an interaction of monomers with RNA-processing factors. Filamentous actin probes recognize discrete structures with submicron lengths that are excluded from chromatin-rich regions. In time-lapse movies, these actin filament structures exhibit one of two types of mobility: 1) diffusive, with an average diffusion coefficient of 0.06–0.08 μm2/s, or (2) subdiffusive, with a mobility coefficient of 0.015 μm2/s. Individual filament trajectories exhibit features of particles moving within a viscoelastic mesh. The small size of nuclear actin filaments is inconsistent with a role in micron-scale intranuclear transport, and their localization suggests that they do not participate directly in chromatin-based processes. Our results instead suggest that actin filaments form part of a large, viscoelastic structure in the nucleoplasm and may act as scaffolds that help organize nuclear contents.This bulk of this work was supported by a grant from the National Institutes of Health to R.D.M. (5R01GM061010-12). Additional support was provided by National Institutes of Health Grant R01 CA096840 (E.H.B.), a National Science Foundation Predoctoral Fellowship (B.B.), a National Institutes of Health Ruth L. Kirschstein Predoctoral Fellowship (B.B.), and a Genentech Fellowship (B.C.)