The single row layout problem with clearances

The single row layout problem (SRLP) is a specially structured instance of the classical facility layout problem, especially used in flexible manufacturing systems. The SRLP consists of finding the most efficient arrangement of a given number of machines along one side of the material handling path with the purpose of minimising the total weighted sum of distances among all machine pairs. To reflect real manufacturing situations, a minimum space (so-called clearances) between machines may be required by observing technological constraints, safety considerations and regulations. This thesis intends to outline the different concepts of clearances used in literature and analyse their effects on modelling and solution approaches for the SRLP. In particular the special characteristics of sequence-dependent, asymmetric clearances are discussed and finally extended to large size clearances (machine-spanning clearances). For this, adjusted and novel model formulations and solution approaches are presented. Furthermore, a comprehensive survey of articles published in this research area since 2000 is provided which identify recent developments and emerging trends in SRLP

Estudo comparativo dos níveis de cortisol salivar e estresse em atletas de luta olímpica de alto rendimento

Orientador : Ricardo Weigert CoelhoDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Educação Física. Defesa: Curitiba, 2006Inclui bibliografiaÁrea de concentração: Exercício e esport

Perfil de qualidade de vida, estresse e algias nos trabalhadores noturnos e diurnos

Orientadora: Neiva LeiteMonografia (licenciatura) - Universidade Federal do Paraná. Setor de Ciências Biológicas. Curso de Educação Físic

Diagnostic value of blood inflammatory markers for detection of acute appendicitis in children

BACKGROUND: Acute appendicitis (AA) is a common surgical problem that is associated with an acute-phase reaction. Previous studies have shown that cytokines and acute-phase proteins are activated and may serve as indicators for the severity of appendicitis. The aim of this study was to compare diagnostic value of different serum inflammatory markers in detection of phlegmonous or perforated appendicitis in children. METHODS: Data were collected prospectively on 211 consecutive children. Laparotomy was performed for suspected AA for 189 patients. Patients were subdivided into groups: nonsurgical abdominal pain, early appendicitis, phlegmonous or gangrenous appendicitis, perforated appendicitis. White blood cell count (WBC), serum C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor α (TNF-α), acid α(1)-glycoprotein (α(1)GP), endotoxin, and erythrocyte sedimentation reaction (ESR) were estimated ad the time of admission. The diagnostic performance was analyzed using receiver operating characteristic (ROC) curves. RESULTS: WBC count, CRP and IL-6 correlated significantly with the severity of appendiceal inflammation. Identification of children with severe appendicitis was supported by IL-6 or CRP but not WBC. Between IL-6 and CRP, there were no significant differences in diagnostic use. CONCLUSION: Laboratory results should be considered to be integrated within the clinical assessment. If used critically, CRP and IL-6 equally provide surgeons with complementary information in discerning the necessity for urgent operation

RELAÇÃO ENTRE A AUTO-ESTIMA, O NÍVEL TÉCNICO, O TEMPO DE PRÁTICA E O RESULTADO DA COMPETIÇÃO DE ATLETAS DE VOLEIBOL FEMININO

A preparação psicológica tem grande importância na performance do atleta e deve fazer parte do treinamento. A auto-estima é um dos fatores que pode influenciar a performance dos atletas. Este estudo tem como objetivo verificar a relação da auto-estima com o nível técnico, o tempo de prática e o resultado da competição de atletas de voleibol feminino da categoria mirim. A amostra foi constituída de 48 atletas, do sexo feminino, com idade de 11 à 13 anos, participantes da X International Cup 2003. Para coleta de dados foram utilizados: um teste diagnóstico, aplicado aos técnicos para analisara as atletas quanto ao nível técnico e auto-estima; e um inventário, aplicada às atletas para analisar a auto-estima. Para análise dos dados foi feita a correlação de Sperman Rho, fixando um nível de significância para alfa em 0,05. Para o tratamento dos dados foi utilizado o programa Statistica 5.5. Verificou-se que a auto-estima apresentou, embora em valores baixos, uma correlação com o resultado da competição, o nível técnico e a auto-estima percebida pelo técnico, com uma diferença estatística significativa. Em relação ao tempo de prática não apresentou diferença significativa. Conclui-se neste estudo que a auto-estima pode ser um dos fatores que influencia no resultado da competição e no nível técnico das atletas. Sugere-se outros estudos sobre o tema, com diferentes populações e variáveis. Abstract The Psychological preparation has a big importance in the performance and has to be part of the training process. Self-esteem is one of the factors that can influence the performance of athletes. This study has as objective to verify the relation between self-esteem, technical level, time of practice and the results in the competition of female volleyball mirim athletes. The sample were composed of 48 athletes, female, with age of 11 to 13 years, that were in the X International Cup - 2003. To collect data, were used: a diagnostic test, to the coaches to analyze the athletes in technical level and self-esteem; and an Inventory to the athletes to selfesteem. The statistic used was, Spearman Ho correlation, in a level of 0,05 to alpha, the program used was Statistica 5.5. Verified that self-esteem presented, in low scores, a correlation with the results of the competition, the technical level and the percept self-esteem, with significant statistic level. In the correlation with time of practice the was not significance. The conclusion of this study, is that self-esteem can be one of the factors that has an influence in the results of the competition and in technical level of the athletes. It suggests others studies about this theme, with different population and variables

Concentric and eccentric endurance exercise reverse hallmarks of T-Cell senescence in pre-diabetic subjects

The peripheral T-cell pool undergoes a striking age associated remodeling which is accelerated by progressive insulin resistance. Exercise training is known to delay several aspects of T-cell senescence. The purpose of the current study was to investigate the effect of 3 weeks regular concentric or eccentric endurance exercise training on the composition of the T-cell compartment in pre-diabetic subjects. Sixteen male older adults with impaired glucose tolerance were recruited and performed either concentric exercise (CE) or eccentric exercise (EE) walking 3 times a week for 3 weeks. Fasting venous blood sampling was performed before training and after the training intervention. Various T-cell subpopulations were analyzed by flow cytometry. We did not find significant time × group effects (interaction) but found several significant time effects for cell type ratios and cell subsets proportions. There was an increase of the CD4+/CD8+ (0.55 ± 0.85%; p = 0.033) and CD4+/CD3+ ratio (5.63 ± 8.44%; p = 0.018) and a decrease of the CD8+/CD3+ ratio (-0.95 ± 1.64%; p = 0.049) after training. We found proportional increases of CD4+/CCR7+/CD45RO+ central memory cells (5.02 ± 7.68%; p = 0.030), naïve CD8+/CCR7+/CD45RO- (3.00 ± 6.68%; p = 0.047) and CD8+/CCR7+/CD45RO+ central memory cells (3.01 ± 3.70%; p = 0.009), while proportions of CD4+/CCR7-/CD45RO- TEMRA cells (-2.17 ± 4.66%; p = 0.012), CD8+/CCR7-/CD45RO- TEMRA cells (-5.11 ± 7.02%; p = 0.018) and CD16+ cells (-4.67 ± 6.45%; p = 0.016) decreased after training. 3 weeks of either CE or EE were effective in reversing hallmarks of T-cell senescence in pre-diabetic subjects. It is suggested that exercise stimulates production and mobilization of naïve T-cells, while differentiated TEMRA cells might disappear by apoptosis

miR-34a as hub of T cell regulation networks

Background: Micro(mi)RNAs are increasingly recognized as central regulators of immune cell function. While it has been predicted that miRNAs have multiple targets, the majority of these predictions still await experimental confirmation. Here, miR-34a, a well-known tumor suppressor, is analyzed for targeting genes involved in immune system processes of leucocytes. Methods: Using an in-silico approach, we combined miRNA target prediction with GeneTrail2, a web tool for Multi-omics enrichment analysis, to identify miR-34a target genes, which are involved in the immune system process subcategory of Gene Ontology. Results: Out of the 193 predicted target genes in this subcategory we experimentally tested 22 target genes and confirmed binding of miR-34a to 14 target genes including VAMP2, IKBKE, MYH9, MARCH8, KLRK1, CD11A, TRAFD1, CCR1, PYDC1, PRF1, PIK3R2, PIK3CD, AP1B1, and ADAM10 by dual luciferase assays. By transfecting Jurkat, primary CD4+ and CD8+ T cells with miR-34a, we demonstrated that ectopic expression of miR-34a leads to reduced levels of endogenous VAMP2 and CD11A, which are central to the analyzed subcategories. Functional downstream analysis of miR-34a over-expression in activated CD8+ T cells exhibits a distinct decrease of PRF1 secretion. Conclusions: By simultaneous targeting of 14 mRNAs miR-34a acts as major hub of T cell regulatory networks suggesting to utilize miR-34a as target of intervention towards a modulation of the immune responsiveness of T-cells in a broad tumor context