Miniaturized Laser Altimeter for Small Satellite Applications

Laser Altimetry is a powerful tool to create absolutely calibrated digital terrain maps of planetary surfaces, to analyze their surface geology, and to get insight into the interior structure of planetary bodies by measuring tidal elevations and libration amplitudes and frequencies. The recent ESA missions BepiColombo and the Jupiter Icy Moons Explorer (JUICE) carry the first European laser altimeter instruments, i.e., the BepiColombo Laser Altimeter (BELA) and the Ganymede Laser Altimeter (GALA), the latter of which has a strong contribution from JAXA teams. The measurement principle of a laser altimeter is very simple. It is based on the time-of-flight measurement of an optical pulse. BELA, which is now on the way to Mercury orbit, applies a diode-laser pumped Nd:YAG laser sending pulses with an energy of 50 mJ, a width of about 5 ns, and a repetition rate of 10 Hz. Over typical ranging distances of 400 km to more than 1000 km, the BELA telescope collects pulses with a few hundred photons and a width of about 25 ns where the time of arrival gives the mean topographic altitude of the area illuminated by the 5 to 40 m diameter laser beam. The return pulse width further gives information on slope and roughness within this area. GALA is a similar instrument with 17 mJ pulse energy but 30 Hz repetition rate and was launched in April 2023 to enter the Jovian system after a eight-year cruise to fly-by at Europa and Callisto and finally orbit the Jovian moon Ganymede at an altitude of about 500 km above its icy surface. BELA and GALA are instruments that consume about 50 W and have a mass of close to 15 kg and 25 kg, respectively. The instrument dimensions are largely determined by the telescope diameter of about 30 cm. In order to enable the use of these instruments on small satellites the size, weight and power (SWaP) budgets need to be drastically reduced. This can be achieved by deriving the time-of-flight information from just a single return photon. The reduction factor of about 100 in the detected photon number can be shared by a reduction in laser energy and a reduction of telescope aperture diameter. Our aim is to reduce laser pulse energy from 17 mJ to 1 mJ and telescope diameter from 22 cm (for GALA) to 8 cm which implies in total a reduction factor about 130. GALA typically detects 700 photons per pulse at an altitude of 500 km which leads to about 5 photons to be analyzed per event by a single photon detection laser altimeter. The major challenges for a single photon detection laser altimeter are the reduction of the background photon rate by reducing the field-of-view of the telescope as well as better spectral filtering. We present first results from a conceptual experimental study of such a system designed for use on small satellites applying a newly developed detection scheme using a Single Photon Avalanche Diode (SPAD) and a diode-laser pumped microchip Nd:YAG laser emitting 1 mJ pulses with a pulse width of 1 ns. The reductions in dimension, mass, and power consumption of this instrument are discussed, and the scientific performance is simulated based on first experimental results. The feasibility of accommodating the instrument on the modular TUBiX20 microsatellite platform developed by Technische Universität Berlin is explored and the necessary requirements for attitude and orbit determination and control as well as SWaP budgets are derived

Serological evidence of influenza a viruses in frugivorous bats from Africa

Bats are likely natural hosts for a range of zoonotic viruses such as Marburg, Ebola, Rabies, as well as for various Corona- and Paramyxoviruses. In 2009/10, researchers discovered RNA of two novel influenza virus subtypes - H17N10 and H18N11 - in Central and South American fruit bats. The identification of bats as possible additional reservoir for influenza A viruses raises questions about the role of this mammalian taxon in influenza A virus ecology and possible public health relevance. As molecular testing can be limited by a short time window in which the virus is present, serological testing provides information about past infections and virus spread in populations after the virus has been cleared. This study aimed at screening available sera from 100 free-ranging, frugivorous bats (Eidolon helvum) sampled in 2009/10 in Ghana, for the presence of antibodies against the complete panel of influenza A haemagglutinin (HA) types ranging from H1 to H18 by means of a protein microarray platform. This technique enables simultaneous serological testing against multiple recombinant HA-types in 5μl of serum. Preliminary results indicate serological evidence against avian influenza subtype H9 in about 30% of the animals screened, with low-level cross-reactivity to phylogenetically closely related subtypes H8 and H12. To our knowledge, this is the first report of serological evidence of influenza A viruses other than H17 and H18 in bats. As avian influenza subtype H9 is associated with human infections, the implications of our findings from

The Ganymede Laser Altimeter (GALA) for the Jupiter Icy Moons Explorer (JUICE): Mission, science, and instrumentation of its receiver modules

The Jupiter Icy Moons Explorer (JUICE) is a science mission led by the European Space Agency, being developed for launch in 2023. The Ganymede Laser Altimeter (GALA) is an instrument onboard JUICE, whose main scientific goals are to understand ice tectonics based on topographic data, the subsurface structure by measuring tidal response, and small-scale roughness and albedo of the surface. In addition, from the perspective of astrobiology, it is imperative to study the subsurface ocean scientifically. The development of GALA has proceeded through an international collaboration between Germany (the lead), Japan, Switzerland, and Spain. Within this framework, the Japanese team (GALA-J) is responsible for developing three receiver modules: the Backend Optics (BEO), the Focal Plane Assembly (FPA), and the Analog Electronics Module (AEM). Like the German team, GALA-J also developed software to simulate the performance of the entire GALA system (performance model). In July 2020, the Proto-Flight Models of BEO, FPA, and AEM were delivered from Japan to Germany. This paper presents an overview of JUICE/GALA and its scientific objectives and describes the instrumentation, mainly focusing on Japan’s contribution

Evidence for Novel Hepaciviruses in Rodents

Hepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research is complicated by a lack of accessible small animal models. The systematic investigation of viruses of small mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled the so-far largest collection of small-mammal samples from around the world, qualified to be screened for bloodborne viruses, including sera and organs from 4,770 roden

Differential sensitivity of bat cells to infection by enveloped RNA viruses: coronaviruses, paramyxoviruses, filoviruses, and influenza viruses.

Bats (Chiroptera) host major human pathogenic viruses including corona-, paramyxo, rhabdo- and filoviruses. We analyzed six different cell lines from either Yinpterochiroptera (including African flying foxes and a rhinolophid bat) or Yangochiroptera (genera Carollia and Tadarida) for susceptibility to infection by different enveloped RNA viruses. None of the cells were sensitive to infection by transmissible gastroenteritis virus (TGEV), a porcine coronavirus, or to infection mediated by the Spike (S) protein of SARS-coronavirus (SARS-CoV) incorporated into pseudotypes based on vesicular stomatitis virus (VSV). The resistance to infection was overcome if cells were transfected to express the respective cellular receptor, porcine aminopeptidase N for TGEV or angiotensin-converting enzyme 2 for SARS-CoV. VSV pseudotypes containing the S proteins of two bat SARS-related CoV (Bg08 and Rp3) were unable to infect any of the six tested bat cell lines. By contrast, viral pseudotypes containing the surface protein GP of Marburg virus from the family Filoviridae infected all six cell lines though at different efficiency. Notably, all cells were sensitive to infection by two paramyxoviruses (Sendai virus and bovine respiratory syncytial virus) and three influenza viruses from different subtypes. These results indicate that bat cells are more resistant to infection by coronaviruses than to infection by paramyxoviruses, filoviruses and influenza viruses. Furthermore, these results show a receptor-dependent restriction of the infection of bat cells by CoV. The implications for the isolation of coronaviruses from bats are discussed

Computational characterization and evaluation of deformation behavior of spherulite of high density polyethylene in mesoscale domain

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum