1,844 research outputs found
How occasional backstepping can speed up a processive motor protein
Fueled by the hydrolysis of ATP, the motor protein kinesin literally walks on
two legs along the biopolymer microtubule. The number of accidental backsteps
that kinesin takes appears to be much larger than what one would expect given
the amount of free energy that ATP hydrolysis makes available. This is puzzling
as more than a billion years of natural selection should have optimized the
motor protein for its speed and efficiency. But more backstepping allows for
the production of more entropy. Such entropy production will make free energy
available. With this additional free energy, the catalytic cycle of the kinesin
can be speeded up. We show how measured backstep percentages represent an
optimum at which maximal net forward speed is achieved.Comment: LaTeX, 5 pages, 3 figure
Recommended from our members
A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus.
Gene-drive systems in diploid organisms bias the inheritance of one allele over another. CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site where the gRNA directs Cas9-mediated cleavage. In the presence of Cas9, the gRNA cassette and any linked cargo sequences are copied via homology-directed repair (HDR) onto the homologous chromosome. Here, we develop an analogous CRISPR-based gene-drive system for the bacterium Escherichia coli that efficiently copies a gRNA cassette and adjacent cargo flanked with sequences homologous to the targeted gRNA/Cas9 cleavage site. This "pro-active" genetic system (Pro-AG) functionally inactivates an antibiotic resistance marker on a high copy number plasmid with ~ 100-fold greater efficiency than control CRISPR-based methods, suggesting an amplifying positive feedback loop due to increasing gRNA dosage. Pro-AG can likewise effectively edit large plasmids or single-copy genomic targets or introduce functional genes, foreshadowing potential applications to biotechnology or biomedicine
Liquid-liquid interfacial tension of electrolyte solutions
It is theoretically shown that the excess liquid-liquid interfacial tension
between two electrolyte solutions as a function of the ionic strength I behaves
asymptotically as O(- I^0.5) for small I and as O(+- I) for large I. The former
regime is dominated by the electrostatic potential due to an unequal
partitioning of ions between the two liquids whereas the latter regime is
related to a finite interfacial thickness. The crossover between the two
asymptotic regimes depends sensitively on material parameters suggesting that,
depending on the actual system under investigation, the experimentally
accessible range of ionic strengths can correspond to either the small or the
large ionic strength regime. In the limiting case of a liquid-gas surface where
ion partitioning is absent, the image chage interaction can dominate the
surface tension for small ionic strength I such that an Onsager-Samaras
limiting law O(- I ln(I)) is expected. The proposed picture is consistent with
more elaborate models and published measurements.Comment: Accepted for publication in Physical Review Letter
Coordinating Humanitarian Entry in the United States and Mexico: A Bilateral Approach to U.S. Legal Migration
Mexico and the United States have stated a joint interest in reducing illegal immigration through Mexico to the U.S.-Mexican border. Both countries are signatories of the Los Angeles Declaration on Migration and Protection, which pledges a coordinated multilateral approach to addressing migration, and Mexico has worked with the United States on its enforcement efforts, accepting returns from the United States. One untapped area of potential coordination is in each nation's authorization for migrants to temporarily enter their countries for humanitarian reasons.Unfortunately, the lack of coordination has meant that many migrants travel through Mexico and congregate in northern Mexico near the U.S.-Mexican border to try to obtain humanitarian entry into the United States. A better approach would be for Mexico to issue cards for visitors for humanitarian reasons at the Guatemalan??Mexican border, allowing migrants to travel to Mexico City, where they could apply for U.S. parole and fly directly to the United States legally
Stability of additive-free water-in-oil emulsions
We calculate ion distributions near a planar oil-water interface within
non-linear Poisson-Boltzmann theory, taking into account the Born self-energy
of the ions in the two media. For unequal self-energies of cations and anions,
a spontaneous charge separation is found such that the water and oil phase
become oppositely charged, in slabs with a typical thickness of the Debye
screening length in the two media. From the analytical solutions, the
corresponding interfacial charge density and the contribution to the
interfacial tension is derived, together with an estimate for the
Yukawa-potential between two spherical water droplets in oil. The parameter
regime is explored where the plasma coupling parameter exceeds the
crystallization threshold, i.e. where the droplets are expected to form
crystalline structures due to a strong Yukawa repulsion, as recently observed
experimentally. Extensions of the theory that we discuss briefly include
numerical calculations on spherical water droplets in oil, and analytical
calculations of the linear PB-equation for a finite oil-water interfacial
width.Comment: 9 pages, 4 figures, accepted by JPCM for proceedings of LMC
Ion-channel-like behavior in lipid bilayer membranes at the melting transition
It is well known that at the gel-liquid phase transition temperature a lipid
bilayer membrane exhibits an increased ion permeability. We analyze the
quantized currents in which the increased permeability presents itself. The
open time histogram shows a "-3/2" power law which implies an open-closed
transition rate that decreases like as time evolves. We
propose a "pore freezing" model to explain the observations. We discuss how
this model also leads to the noise that is commonly observed in
currents across biological and artificial membranes.Comment: 5 pages, 4 figure
Information and maximum power in a feedback controlled Brownian ratchet
Closed-loop or feedback controlled ratchets are Brownian motors that operate
using information about the state of the system. For these ratchets, we compute
the power output and we investigate its relation with the information used in
the feedback control. We get analytical expressions for one-particle and
few-particle flashing ratchets, and we find that the maximum power output has
an upper bound proportional to the information. In addition, we show that the
increase of the power output that results from changing the optimal open-loop
ratchet to a closed-loop ratchet also has an upper bound that is linear in the
information.Comment: LaTeX, 6 pages, 4 figures, improved version to appear in Eur. Phys.
J.
Self diffusion of particles in complex fluids: temporary cages and permanent barriers
We study the self diffusion of individual particles in dense (non-)uniform
complex fluids within dynamic density functional theory and explicitly account
for their coupling to the temporally fluctuating background particles. Applying
the formalism to rod-like particles in uniaxial nematic and smectic liquid
crystals, we find correlated diffusion in different directions: The temporary
cage formed by the neighboring particles competes with permanent barriers in
periodic inhomogeneous systems such as the lamellar smectic state and delays
self diffusion of particles even in uniform systems. We compare our theory with
recent experimental data on the self diffusion of fluorescently labelled
filamentous virus particles in aqueous dispersions in the smectic phase and
find qualitative agreement. This demonstrates the importance of explicitly
dealing with the time-dependent self-consistent molecular field that every
particle experiences.Comment: submitte
Spontaneous Charging and Crystallization of Water Droplets in Oil
We study the spontaneous charging and the crystallization of spherical
micron-sized water-droplets dispersed in oil by numerically solving, within a
Poisson-Boltzmann theory in the geometry of a spherical cell, for the density
profiles of the cations and anions in the system. We take into account
screening, ionic Born self-energy differences between oil and water, and
partitioning of ions over the two media. We find that the surface charge
density of the droplet as induced by the ion partitioning is significantly
affected by the droplet curvature and by the finite density of the droplets. We
also find that the salt concentration and the dielectric constant regime in
which crystallization of the water droplets is predicted is enhanced
substantially compared to results based on the planar oil-water interface,
thereby improving quantitative agreement with recent experiments.Comment: 10 pages, 7 figures, submitted for publicatio
- …