Telemedicine in cardiology

Introduction Telemedicine is becoming regularly used in rural part of some of developed countries. Moreover, it could be applied to improve diagnosis and emergency care in adults as well as in neonatal care. Material and methods Articles in the EBSCO database have been analyzed using keywords: telemedicine, cardiology. The available literature is subjectively selected. Then, the newest version of every paper was searched for. Results Results of researches with applications of telemedicine in cardiology in emergency, chronic conditions. Moreover, potential costs and benefits were described. Conclusions Telemedicine could be useful for patients, their families, clinicians and regional healthcare systems. The rapidly changing healthcare climate includes an increasing focus on cost reduction, quality improvement and results, patient-centered care and technology solutions, all of which are well served by the optimal use of telemedicine

Geoproduct potential analysis based on the example of the GEOsfera Ecological and Geological Education Center in Jaworzno

At the time of discovering and exposing the scientific and educational potential of areas where mineral resources were exploited in the past, they have become a significant element in tourism elements. In the presented paper, the potential of the GEOsfera Ecological and Geological Education Centre in Jaworzno as a geoproduct was analysed. In the first part of the study, based on the published works and consultations with experts, the “geoproduct” was defined. Then the area, where the GEOsphere is located, was characterized. Its geology, the natural world, the way of land development and the promotion of geo-attractions located in this area are described. In the next stage, an analysis of the opinions of users regarding their perception of the GEOsphere was carried out. For this purpose, evaluations posted on social media and the collected survey results were used. As shown by the results, the majority of users of the GEOsfera are residents of Jaworzno – 63% of the respondents. The importance of the GEOsphere as an educational centre was indicated by 22% of visitors. About 5% of the respondents mentioned learning and obtaining information from the descriptions placed next to the exhibits in the centre. 93% of respondents recognised the graduation tower as the greatest attraction of the GEOsphere. Over 92% of people noticed the recreational role of the GEOsphere. 81% of users declared that they were satisfied with their visit to the GEOsphere. More than half of the respondents (56%) visited the GEOsfera with their family. A significant part of respondents (42%), pointed to the role of environmental protection, which is extremely important from the point of view of geoproducts’ design. The respondents also indicated that the GEOsphere has numerous geological, natural and educational functions and protects and promotes the geological heritage of the region

Telemedicine in neurology

Introduction Telemedicine in neurology has been developed for the last two decades. However, this concept has only been introduced to the emergency scene recently. In the above review current state and prognosis of development of telemedicine would be described. Material and methods Articles in the EBSCO database have been analyzed using keywords: telemedicine, neurology. The available literature is subjectively selected. Then, the newest version of every paper was searched for. Results Results of researches with applications of telemedicine in neurology in emergency, chronic conditions. Moreover, potential costs and benefits were described. Conclusions The involvement of stroke specialists also facilitated the diagnosis of other neurological emergencies such as intracerebral hemorrhage, subarachnoid hemorrhage, hypoglycemia and seizure. Potential barriers to the use of this technology include the initial costs of equipment and training of personnel. Further development of telemedicine would possibly improve both emergency and long-term care in neurology

Long Time Scale Ensemble Methods in Molecular Dynamics: Ligand–Protein Interactions and Allostery in SARS-CoV-2 Targets

We subject a series of five protein-ligand systems which contain important SARS-CoV-2 targets, 3-chymotrypsin-like protease (3CLPro), papain-like protease, and adenosine ribose phosphatase, to long time scale and adaptive sampling molecular dynamics simulations. By performing ensembles of ten or twelve 10 μs simulations for each system, we accurately and reproducibly determine ligand binding sites, both crystallographically resolved and otherwise, thereby discovering binding sites that can be exploited for drug discovery. We also report robust, ensemble-based observation of conformational changes that occur at the main binding site of 3CLPro due to the presence of another ligand at an allosteric binding site explaining the underlying cascade of events responsible for its inhibitory effect. Using our simulations, we have discovered a novel allosteric mechanism of inhibition for a ligand known to bind only at the substrate binding site. Due to the chaotic nature of molecular dynamics trajectories, regardless of their temporal duration individual trajectories do not allow for accurate or reproducible elucidation of macroscopic expectation values. Unprecedentedly at this time scale, we compare the statistical distribution of protein-ligand contact frequencies for these ten/twelve 10 μs trajectories and find that over 90% of trajectories have significantly different contact frequency distributions. Furthermore, using a direct binding free energy calculation protocol, we determine the ligand binding free energies for each of the identified sites using long time scale simulations. The free energies differ by 0.77 to 7.26 kcal/mol across individual trajectories depending on the binding site and the system. We show that, although this is the standard way such quantities are currently reported at long time scale, individual simulations do not yield reliable free energies. Ensembles of independent trajectories are necessary to overcome the aleatoric uncertainty in order to obtain statistically meaningful and reproducible results. Finally, we compare the application of different free energy methods to these systems and discuss their advantages and disadvantages. Our findings here are generally applicable to all molecular dynamics based applications and not confined to the free energy methods used in this study

Minor chemistry changes alter surface hydration to control fibronectin adsorption and assembly into nanofibrils

Fibronectin (FN) is a large glycoprotein which links and transmits signals between the cell's cytoskeleton and the extracellular matrix. FN organization into fibrils and then fibrillogenesis can be induced with the right substrate, such as poly(ethyl acrylate) (PEA), on which FN becomes extended. Interestingly, the almost identical polymer poly(methyl acrylate) (PMA), which has one less methylene bridge (─CH2─), does not cause fibrillogenesis. To investigate the difference in FN behavior on PEA and PMA, the two substrates are modeled using ethyl acrylate (EA) and methyl acrylate (MA) functionalized self‐assembled monolayers (SAMs). It is confirmed experimentally that the EA and MA SAMs exhibit a similar behavior in vitro to the polymers in terms of fibronectin fibrillogenesis, domain exposure, and cell adhesion. All‐atom molecular dynamics simulations of the FNIII 9‐10 domains interacting with each SAM show the adsorption of these two domains on EA SAMs and no adsorption on MA SAMs. Consistently, the experiments show that FN fibrillogenesis takes place on EA SAMs but not on MA SAMs. It is found that the extra methylene group in the EA headgroup leads to more motion within the headgroup that results in a markedly less dense hydration layer, which facilitates FN fibrillogenesis

Geoproduct potential analysis based on the example of the GEOsfera Ecological and Geological Education Center in Jaworzno

At the time of discovering and exposing the scientific and educational potential of areas where mineral resources were exploited in the past, they have become a significant element in tourism elements. In the presented paper, the potential of the GEOsfera Ecological and Geological Education Centre in Jaworzno as a geoproduct was analysed. In the first part of the study, based on the published works and consultations with experts, the “geoproduct” was defined. Then the area, where the GEOsphere is located, was characterized. Its geology, the natural world, the way of land development and the promotion of geo-attractions located in this area are described. In the next stage, an analysis of the opinions of users regarding their perception of the GEOsphere was carried out. For this purpose, evaluations posted on social media and the collected survey results were used. As shown by the results, the majority of users of the GEOsfera are residents of Jaworzno – 63% of the respondents. The importance of the GEOsphere as an educational centre was indicated by 22% of visitors. About 5% of the respondents mentioned learning and obtaining information from the descriptions placed next to the exhibits in the centre. 93% of respondents recognised the graduation tower as the greatest attraction of the GEOsphere. Over 92% of people noticed the recreational role of the GEOsphere. 81% of users declared that they were satisfied with their visit to the GEOsphere. More than half of the respondents (56%) visited the GEOsfera with their family. A significant part of respondents (42%), pointed to the role of environmental protection, which is extremely important from the point of view of geoproducts’ design. The respondents also indicated that the GEOsphere has numerous geological, natural and educational functions and protects and promotes the geological heritage of the region. W dobie odkrywania i eksponowania potencjału naukowego i edukacyjnego terenów, na których w przeszłości prowadzono eksploatację surowców, stają się one znaczącymi obiektami turystycznymi. W prezentowanej pracy dokonano analizy potencjału Centrum Edukacji Ekologiczno-Geologicznej GEOsfera w Jaworznie, zlokalizowanego na terenie wyrobiska surowców skalnych, jako geoproduktu. W pierwszej części opracowania, opierając się na dostępnych pracach publikowanych i konsultacjach z ekspertami, zdefiniowano „geoprodukt” oraz określono kryteria, jakie powinien spełniać. Następnie opisano teren, na którym zlokalizowana jest GEOsfera, uwzględniając geologię, elementy przyrody ożywionej oraz sposób zagospodarowania terenu i promowania geoatrakcji, które się tam znajdują. Na kolejnym etapie przeprowadzono analizę opinii użytkowników w zakresie postrzegania przez nich GEOsfery. Do tego celu wykorzystano oceny zamieszczone w mediach społecznościowych i zgromadzone wyniki badań ankietowych. Wykazały one, że większość spośród badanych użytkowników GEOsfery to mieszkańcy Jaworzna, którzy stanowili 63% ankietowanych. Znaczenie GEOsfery jako istotnego ośrodka edukacyjnego wskazało 22% odwiedzających. Około 5% pytanych doceniło uczenie się i pozyskiwanie informacji z opisów umieszczonych na tablicach obok eksponatów na terenie ośrodka. Jako największą atrakcję terenu GEOsfery 93% ankietowanych wskazało tężnię solankową. Ponad 92% respondentów dostrzegło rekreacyjną rolę GEOsfery i dogodne warunki do aktywnego spędzania czasu. 81% badanych zadeklarowało zadowolenie z wizyty. Ponad połowa respondentów (56%) odwiedziła GEOsferę z rodziną. Znaczna część ankietowanych (42%) zwróciła uwagę na znaczenie ochrony środowiska w funkcjonowaniu GEOsfery, co jest niezwykle ważną informacją przydatną podczas projektowania geoproduktu. Użytkownicy, którzy wzięli udział w badaniu, wskazali również, że GEOsfera ma liczne walory geologiczne, przyrodnicze i edukacyjne oraz chroni i promuje dziedzictwo geologiczne regionu

ACE and ACE2 expression in normal and malignant skin lesions

 The renin-angiotensin system (RAS) is known mainly as a regulator of cardiovascular homeostasis. However, it has also been shown to mediate processes such as proliferation, apoptosis, angiogenesis, and carcinogenesis. Nonmelanoma skin cancers (NMSC) — including basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) — are among the most common cancers. The aim of the present study was to determine the immunohistochemical expression of angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), and Ki-67 antigen in archival samples of normal skin, actinic keratosis, and malignant skin lesions. Cytoplasmic-nuclear ACE immunoreactivity was observed in 99% of examined cases of both normal skin and cancers. Significantly higher ACE immunoreactivity occurred in normal skin, as compared with BCC and SCC (p < 0.01, p < 0.0001, respectively). Additionally, ACE immunoreactivity was also significantly higher in BCC, compared with SCC (p < 0.05). ACE2 immunoreactivity was noted in basal epidermal layers and in sebaceous gland cells in normal skin, though not in NMSC. These novel observations suggest that ACE and skin RAS may be involved in the pathogenesis of malignant skin lesions.

Breast cancer as a significant social problem

Background: This article is devoted to the topic of breast cancer, which is a very important and overlooked problem by many women. This cancer is the most common malignancy in women in developed countries. It also creates an increasing problem in developing countries and causes high mortality. Early diagnoses of neoplastic lesions and rapid implementation of therapy in most cases allow for successful treatment its prognosis. Self-control is very important, women should examine their breasts by palpation. Further research to diagnose breast cancer are: mammography (MMG), ultrasonography (USG), magnetic resonance (MR), positron emission tomography (PET) and microscopic examination. Material and Methods: In this article, it was analyzed by the latest literature on risk factors, epidemiology, diagnosis and treatment of breast cancer. Articles were searched from PubMed and Google Scholar. Results: Breast cancer risk factors have been shown to be early menstruation, high women's height, high body mass (especially fat content) and hyperinsulinaemia. In addition, genetic factors play an important role. Research also confirms that highly-used cleaners, and at their head, DDP (dichlorodiphenyltrichloroethane) affect the formation of breast cancer. This is the third most common cause of death in women aged 60-85. In treatment, an individual approach to each patient is important. Older women individually discuss the methods of treatment with the doctor, because it gives beneficial results of therapy. Conclusions: Breast cancer has become a very important medical and social problem in older women. Mass media are needed to disseminate knowledge, topics related to treatment and to support the sick. In older women, treatment is more aggressive, and in addition to radiotherapy, a partial mastectomy is performed. Breast cancer is a tought term for woman’s in all age. It is related with fear and loss of self—confidence

Nutritional and dietary interventions for the treatment of Chronic Fatigue Syndrome

Background: Chronic fatigue syndrome is an increasingly frequent disorder characterized by prolonged fatigue and few specific symptoms. He favors prolonged stress and life in a constant hurry. The main symptoms of chronic fatigue include: headache, throat, myalgia, joint pain, short-term memory impairment, sleep disorders, malaise occurring after physical exertion and lasting over 24 hours, and painful lymph nodes. The possibility of chronic fatigue syndrome should be considered in patients with the above symptoms, if they persist for more than 6 months. In this review, we wanted to focus on the possibilities to minimize the symptoms of chronic fatigue syndrome through nutritional and dietary intervention. Materials and methods: Analysis of articles in the EBSCO and Google Scholar database using keywords: Chronic Fatique Syndrome, nutritional, dietary interventions, treatment. Results: Patients with chronic fatigue syndrome suffer from chronic fatigue and not specific symptoms, which makes diagnosis difficult. The analysis of articles shows that dietary and nutritional interventions tailored to the patient reduce the feeling of fatigue, which is the main problem among patients with chronic fatigue syndrome. Positive effects in the form of alleviation of symptoms have been observed while using, inter alia: polyphenols, L-carnitine, probiotics, CoQ10 and NADH and GAA. Conclusions: Dietary and nutritional intervention has a positive effect on intestinal microflora, intestinal mucosa, oxidative stress and proper functioning of mitochondria, thanks to which it reduces symptoms of chronic fatigue syndrome. Individual and appropriate supplementation depending on the etiology of the syndrome can lead to relief of symptoms