Anthropological foundations of the Christian perfection according to the teaching of bishop Edward Kisiel

This article presents the preaching of Bishop Edward Kisiel about the anthropological foundation of the Christian perfection. The Bishop shows man as an individual created in love for love, and thus exalted by God. He also emphasizes the fact of contamination of human nature, which manifests itself in the human inclination to evil. The man appears to be totally dependent on God in all things, so he is not able to overcome the effects of the original sin. Kisiel describes redemption as the most important event in human history. It is a gift and a task for mankind. The Bishop points to the need for cooperation with grace in pursuit of eternal life.Zespół Szkół Katolickich im. Matki Bożej Miłosierdzia w Białymstok

Ethanol behaviour at the solution-air interface in the presence of Triton X-100 and cetyltrimethylammonium bromide mixture

On the basis of the surface tension ( LV g ) data of the aqueous solutions of p-(1,1,3,3-tetramethylbutyl)phenoxypoly(ethylene glycol) (Triton X-100 or TX-100) and cetyltrimethylammonium bromide (CTAB) mixture with ethanol published in our previous paper, the process of ethanol adsorption was investigated. For that reason, the values of Gibbs surface excess concentration of ethanol at the solution-air interface and Gibbs standard free energy of its adsorption at that interface were calculated and compared with those determined for the aqueous solutions of ethanol. The surface excess concentration of ethanol at the solution-air interface was calculated with two different methods. The standard free energy of alcohol adsorption was determined from both the Gu and Zhu and Langmuir equations

Comparison between surface and volumetric properties of short-chain alcohols and some classical surfactants

Measurements of the dynamic surface tension of the aqueous solutions of methanol, ethanol, propanol, CTAB and SDDS at their given concentrations were made. From the obtained results and the literature data it was concluded that the adsorption of short-chain alcohols at the water-air interface is somewhat similar to that of classical surfactants. For that reason the relationship between the Gibbs standard free energy of adsorption of short-chain alcohols and classical surfactants at that interface was established. The correlation between the chemical potential of mixing of alcohols and surfactants was also analysed. This analysis concerned the critical aggregation concentration (CAC) of alcohols and the critical micelle concentration (CMC) of surfactants. The chemical potential of surfactant mixing was calculated from the literature CMC data for the homologous series of alkyl sulfates, alkyl sulfonates, alkyl ammonium chlorides, alkyl trimethylammonium bromides, and alkyl pyridinium bromides. The influence of the hydrophobic chain length of alcohol and surfactant molecules on the Gibbs standard free energy of their adsorption at the water-air interface and their chemical potential of mixing were considered. It appeared that there is a linear dependence between these thermodynamic functions and the number of carbon atoms increased by 1 in the hydrocarbon chains of these compounds. This confirms clearly our conclusion that the behaviour of short-chain alcohols and classical surfactants at the water-air interface and in the bulk phase of aqueous solutions is similar.

Cytotoxic effect and molecular docking of 4-ethoxycarbonylmethyl-1-(piperidin-4-ylcarbonyl)-thiosemicarbazide—a novel topoisomerase II inhibitor

The preliminary cytotoxic effect of 4-ethoxycarbonylmethyl-1-(piperidin-4-ylcarbonyl)-thiosemicarbazide hydrochloride (1)—a potent topoisomerase II inhibitor—was measured using a MTT assay. It was found that the compound decreased the number of viable cells in both estrogen receptor-positive MCF-7 and estrogen receptor-negative MDA-MB-231breast cancer cells, with IC(50) values of 146 ± 2 and 132 ± 2 μM, respectively. To clarify the molecular basis of the inhibitory action of 1, molecular docking studies were carried out. The results suggest that 1 targets the ATP binding pocket. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00894-012-1679-6) contains supplementary material, which is available to authorized users

Cytotoxic efficacy of a novel dinuclear platinum(II) complex used with anti-MUC1 in human breast cancer cells

Mucin 1 (MUC1) is overexpressed in various cancer cells especially in breast cancer cells. There are known research works on the use of anti-MUC1 antibody with docetaxel in ovarian cancer, but there are no data about combined therapy platinum compounds with anti-MUC1 in breast cancer. The aim of the study was to evaluate the antiproliferative properties of a new dinuclear platinum(II) complex (Pt12) used with anti-MUC1 in human breast cancer cells. The dinuclear platinum(II) complex (Pt12) has been synthesized, and its cytotoxicity with anti-MUC1 has been tested in both MCF-7 and MDA-MB-231 breast cancer cells. In this study, the effects of Pt12 with anti-MUC1 on collagen and DNA biosynthesis in human breast cancer cells were compared to those evoked by cisplatin and cisplatin with anti-MUC1. The mechanism of action of Pt12 with anti-MUC1 was studied employing flow cytometry assessment of annexin V binding assay. It was found that Pt12 with anti-MUC1 was more active inhibitor of DNA and collagen synthesis as well more cytotoxic agent than Pt12 alone and cisplatin with anti-MUC1. Cytotoxicity of Pt12 with anti-MUC1 against breast cancer cells is due to apoptotic cell death as well as necrotic cell death. These results indicate that the use of Pt12 with anti-MUC1 may constitute a novel strategy in the chemotherapy of breast cancer tumors

Analysis of health condition of elderly patients hospitalized in a surgical ward. Analiza stanu odżywienia osób w podeszłym wieku hospitalizowanych w oddziale chirurgicznym.

StreszczenieWstęp. Starzenie się społeczeństwa to poważny problem współczesnego świata. Z procesem tym często wiąże się nieprawidłowy stan odżywienia, a to z kolei ma negatywny wpływ na stan zdrowia osób w podeszłym wieku. W związku z tym bardzo ważna jest ocena stanu odżywienia osób starszych.Cel badań. Celem badań była ocena stanu odżywienia osób starszych hospitalizowanych w oddziale chirurgicznym.Materiał i metody: Badaniem objęto 55 chorych (27 kobiet i 28 mężczyzn), przyjętych w Oddział Chirurgii Ogólnej, Onkologicznej i Kolorektalnej Wielospecjalistycznego Szpitala Miejskiego im. Józefa Strusia w Poznaniu. Badane osoby były hospitalizowane w celach diagnostycznych lub oczekiwały na zabieg operacyjny. Do przeprowadzenia badania zastosowano kwestionariusz MNA.Wyniki. Analiza uzyskanych wyników wyłoniła 47% (26 osób) z ryzykiem niedożywienia i 53% (29 osób) z prawidłowym stanem odżywienia. Nieprawidłowy stan odżywienia dotyczył głównie kobiet. Wszystkie osoby w wieku 85 lat i więcej znalazły się w grupie ryzyka niedożywienia. Negatywny wpływ na stan odżywienia miały również choroby współistniejące takie, jak choroba nowotworowa i depresja.   Wnioski:Istnieje uzasadniona potrzeba monitorowania stanu odżywienia u osób starszych, ponieważ u wielu z  nich występuje ryzyko niedożywienia.Na stan odżywienia osób w podeszłym wieku wpływa wiele czynników zarówno zewnętrznych  jak i wewnętrznych. Ryzyko niedożywienia wzrasta wraz z wiekiem pacjentów.  Słowa kluczowe: stan odżywienia, niedożywienie, osoby starsz

Myonectin serum concentration changes after short-term physical activity among young, healthy people

Background: Myonectin is a myokine secreted by skeletal muscles in response to physical activity (PhA)in rodents. It was shown that myonectin may be positively associated with insulin resistance parameters.The aim of the study was to evaluate changes in the concentration of myonectin after short-term PhA.Methods: A total of 29 young, healthy volunteers, were included in the study. Each participant completeda life-style questionnaire, underwent a physical examination with anthropometric measurement followedby a treadmill test according to theBruce protocol. Blood samples were collected before and after PhA.An ELISA Assay was used to investigate the myonectin serum level.Results: The myonectin serum level did not change significantly after PhA (0.62[0.14-2.9] vs. 1.08[0.15-2.44] ng/ml; p=0.84). Before PhA the myonectin serum level differed significantly between men and women (respectively: 3.92[2.24-5.30] vs. 0.56[0.15-1.75] ng/ml; p=0.02). Before PhA it had a positive association with weight, BMI, serum creatinine and uremic acid (p < 0.05). The change in the level of myonectin serum after PhA had negative associations with weight, BMI, fasting insulin level and HOMA-IR (p < 0.05).Conclusions: Myonectin serum concentration does not change after short-term physical activity amongyoung, healthy people. Changes in the myonectin serum level after short-term physical activity may beassociated with fasting insulin resistance

Evaluation of concentrations of metalloproteinase 2 (MMP-2) and metalloproteinase 9 (MMP-9) in serum of patients with primary hypertension

Wstęp: Metaloproteinazy macierzy zewnątrzkomórkowej (MMP) odgrywają istotną rolę w procesie patologicznej przebudowy ściany naczyń krwionośnych w przebiegu nadciśnienia tętniczego. Głównym celem pracy była ocena stężenia metaloproteinazy 2 i metaloproteinazy 9 w surowicy chorych na pierwotne nadciśnienie tętnicze. Materiał i metody: Badanie wykonano u 38 chor ych na pier wotne nadciśnienie tętnicze, w wieku od 21 do 69 lat (21 mężczyzn i 17 kobiet). Grupę kontrolną stanowiło 11 zdrowych ochotników. Oznaczenia stężenia metaloproteinaz MMP-2 i MMP-9 wykonano techniką ELISA, pr zy zastosowaniu testów fir my R&D. Pozostałe badania biochemiczne wykonano korzystając z testów i analizatorów firmy Siemens. Uzyskane wyniki parametrów klinicznych i badań laboratoryjnych poddano analizie statystycznej, za pomocą programu komputerowego STATISTICA v.10. Wyniki: Stężenia MMP-2 w surowicy chorych na nadciśnienie tętnicze nie różniły istotnie statystycznie w porównaniu z wielkością tych parametrów u osób zdrowych. Stężenia MMP-9 były wyższe u chorych na pierwotne nadciśnienie tętnicze, jednak różnica ta nie była statystycznie znamienna. Uzyskano istotną korelację pomiędzy stężeniem MMP-9 a stężeniem homocysteiny. Wnioski: Ocena stężenia MMP-2 i MMP-9 w surowicy chor ych na pier wotne nadciśnienie tętnicze z zaburzeniami metabolicznymi wymaga dalszych badań z udziałem większe j liczby pacjentów.Introduction: Matrix metalloproteinases play important role in cardiovascular remodeling process in patients with arterial hypertension. The main purpose of this work was the assessment of concentrations of metalloproteinase 2 and metalloproteinase 9 in ser um of patients with primary hypertension. Material and methods: The concentrations of homocysteine, MMP-2 and MMP-9 were measured in 38 patients with primary hypertension at the age of 21–69 (21 male and 17 female). The control group consisted of 11 healthy persons. The concentrations of MMP-2 and MMP-9 were assessed in serum by Elisa method (of sandwich type) using R&D Systems. Other laboratory parameters were determined with use of Siemens method and analyzers. Statistical analysis of results was made with use of STATISTICA v. 10 software. Results: The concentration of MMP -2 in ser um of patients with primar y hyper tension was comparable to concentrations of MMP-2 in persons from control group. MMP-9 concentration was insignificantly higher in serum of patients with primary hypertension compared with patients from control group. Significant correlations were observed between the MMP-9 concentration in serum of patients with primary hypertension and homocysteine concentration. Conclusions: Determination of the role of metalloproteinases MMP -2 and MMP-9 in the development of the arterial hypertension requires further research in larger number of patients