Follow-up monitoring in a cat with leishmaniosis and coinfections with Hepatozoon felis and ‘Candidatus Mycoplasma haemominutum’

Case summary A 6-year-old female neutered domestic shorthair cat from Cyprus was presented with multiple ulcerated skin nodules. Cytology and histopathology of the lesions revealed granulomatous dermatitis with intracytoplasmic organisms, consistent with amastigotes of Leishmania species. Biochemistry identified a mild hyperproteinaemia. Blood extraction and PCR detected Leishmania species, Hepatozoon species and ‘Candidatus Mycoplasma haemominutum’ (CMhm) DNA. Subsequent sequencing identified Hepatozoon felis. Additionally, the rRNA internal transcribed spacer 1 locus of Leishmania infantum was partially sequenced and phylogeny showed it to cluster with species derived from dogs in Italy and Uzbekistan, and a human in France. Allopurinol treatment was administered for 6 months. Clinical signs resolved in the second month of treatment with no deterioration 8 months post-treatment cessation. Quantitative PCR and ELISA were used to monitor L infantum blood DNA and antibody levels. The cat had high L infantum DNA levels pretreatment that gradually declined during treatment but increased 8 months post-treatment cessation. Similarly, ELISA revealed high levels of antibodies pretreatment, which gradually declined during treatment and increased slightly 8 months post-treatment cessation. The cat remained PCR positive for CMhm and Hepatozoon species throughout the study. There was no clinical evidence of relapse 24 months post-treatment. Relevance and novel information To our knowledge, this is the first clinical report of a cat with leishmaniosis with H felis and CMhm coinfections. The high L infantum DNA levels post-treatment cessation might indicate that although the lesions had resolved, prolonged or an alternative treatment could have been considere

The FAOSTAT database of greenhouse gas emissions from agriculture

Peer reviewedPublisher PD

Borrelia persica infection in dogs and cats: clinical manifestations, clinicopathological findings and genetic characterization

Background: Relapsing fever (RF) is an acute infectious disease caused by arthropod-borne spirochetes of the genus Borrelia. The disease is characterized by recurrent episodes of fever that concur with spirochetemia. The RF borrelioses include louse-borne RF caused by Borrelia recurrentis and tick-borne endemic RF transmitted by argasid soft ticks and caused by several Borrelia spp. such as B. crocidurae, B. coriaceae, B. duttoni, B. hermsii, B. hispanica and B. persica. Human infection with B. persica is transmitted by the soft tick Ornithodoros tholozani and has been reported from Iran, Israel, Egypt, India, and Central Asia. Methods: During 2003-2015, five cats and five dogs from northern, central and southern Israel were presented for veterinary care and detected with borrelia spirochetemia by blood smear microscopy. The causative infective agent in these animals was identified and characterized by PCR from blood and sequencing of parts of the flagellin (flab), 16S rRNA and glycerophosphodiester phosphodiestrase (GlpQ) genes. Results: All animals were infected with B. persica genetically identical to the causative agent of human RF. Phylogenetic analysis indicated that DNA sequences from these pet carnivores clustered together with B. persica genotypes I and II from humans and O. tholozani ticks and distinctly from other RF Borrelia spp. The main clinical findings in cats included lethargy, anorexia, anemia in 5/5 cats and thrombocytopenia in 4/5. All dogs were lethargic and anorectic, 4/5 were febrile and anemic and 3/5 were thrombocytopenic. Three dogs were co-infected with Babesia spp. The animals were all treated with antibiotics and the survival rate of both dogs and cats was 80 %. The cat and dog that succumbed to disease died one day after the initiation of antibiotic treatment, while survival in the others was followed by the rapid disappearance of spirochetemia. Conclusions: This is the first report of disease due to B. persica infection in cats and the first case series in dogs. Infection was associated with anemia and thrombocytopenia. Fever was more frequently observed in dogs than cats. Domestic canines and felines suffer from clinical disease due to B. persica infection and may also serve as sentinels for human infection

Framework for assessing sustainability levels in Belgium agricultural systems - SAFE

Sustainability is now regarded as a crucial property of agricultural systems and its evaluation has become a main challenge for scientists, policy makers and farmers. In the last decade, different sets of indicators have been designed both at national and international levels. Meanwhile, more practical environmental impact assessment (EIA) tools have been developed at the farm level . However, none of these indicator sets can be used at both levels. Further, most of these initiatives focus only on environmental aspects of sustainability and do not take socio-economic aspects into consideration. Indicator selection does not always fit in a consistent and comprehensive framework, although there is a strong need to integrate sustainability indicators in order to facilitate comparison and assessment. Finally, few of these works relate to Belgian agriculture, which up til now lacked a tool for assessing the sustainability of its farms. This project aims at providing a framework for assessing sustainability levels in Belgian agricultural systems (SAFE) that overcomes the deficiencies mentioned above. This is achieved by: 1. Considering the concept of agricultural sustainability in a holistic manner – SAFE accounts for all three pillars of sustainability (environmental, economic & social). 2. Developing (a) a consistent approach for defining sustainability principles and criteria and (b) a core list of sustainability indicators identified through a standardized selection procedure. The ‘SAFE selection procedure’ is a flexible scientific process that builts on knowledge and experience of numerous experts. 3. Ensuring that the tool remains as easy as possible to interpret and thus to use, thanks to the integration procedure of sustainability indicators and the graphic expression of the results. 4. Building on a generic methodology. Though the set of selected indicators presented in this report is specific to the Belgian agricultural context, the method developed for the construction of the SAFE tool can be transferred for assessing sustainability levels in other geographical (Europe, world, …) and sectorial contexts. In particular, principles and criteria defined in SAFE have a universal value. 5. Taking action at three spatial levels, depending on the scale of application: (1) parcel (2) farm or (3) watershed for surface water-related issues, landscape/ecosystem for some soil and biodiversity related issues, and administrative units (region, state) for some environmental as well as for some socio-economic issues

Framework for assessing sustainability levels in Belgium agricultural systems - SAFE

Sustainability is now regarded as a crucial property of agricultural systems and its evaluation has become a main challenge for scientists, policy makers and farmers. In the last decade, different sets of indicators have been designed both at national and international levels. Meanwhile, more practical environmental impact assessment (EIA) tools have been developed at the farm level . However, none of these indicator sets can be used at both levels. Further, most of these initiatives focus only on environmental aspects of sustainability and do not take socio-economic aspects into consideration. Indicator selection does not always fit in a consistent and comprehensive framework, although there is a strong need to integrate sustainability indicators in order to facilitate comparison and assessment. Finally, few of these works relate to Belgian agriculture, which up til now lacked a tool for assessing the sustainability of its farms. This project aims at providing a framework for assessing sustainability levels in Belgian agricultural systems (SAFE) that overcomes the deficiencies mentioned above. This is achieved by: 1. Considering the concept of agricultural sustainability in a holistic manner – SAFE accounts for all three pillars of sustainability (environmental, economic & social). 2. Developing (a) a consistent approach for defining sustainability principles and criteria and (b) a core list of sustainability indicators identified through a standardized selection procedure. The ‘SAFE selection procedure’ is a flexible scientific process that builts on knowledge and experience of numerous experts. 3. Ensuring that the tool remains as easy as possible to interpret and thus to use, thanks to the integration procedure of sustainability indicators and the graphic expression of the results. 4. Building on a generic methodology. Though the set of selected indicators presented in this report is specific to the Belgian agricultural context, the method developed for the construction of the SAFE tool can be transferred for assessing sustainability levels in other geographical (Europe, world, …) and sectorial contexts. In particular, principles and criteria defined in SAFE have a universal value. 5. Taking action at three spatial levels, depending on the scale of application: (1) parcel (2) farm or (3) watershed for surface water-related issues, landscape/ecosystem for some soil and biodiversity related issues, and administrative units (region, state) for some environmental as well as for some socio-economic issues

In Vivo and Ex Vivo Evaluation of L-Type Calcium Channel Blockers on Acid β-Glucosidase in Gaucher Disease Mouse Models

Gaucher disease is a lysosomal storage disease caused by mutations in acid β-glucosidase (GCase) leading to defective hydrolysis and accumulation of its substrates. Two L-type calcium channel (LTCC) blockers—verapamil and diltiazem—have been reported to modulate endoplasmic reticulum (ER) folding, trafficking, and activity of GCase in human Gaucher disease fibroblasts. Similarly, these LTCC blockers were tested with cultured skin fibroblasts from homozygous point-mutated GCase mice (V394L, D409H, D409V, and N370S) with the effect of enhancing of GCase activity. Correspondingly, diltiazem increased GCase protein and facilitated GCase trafficking to the lysosomes of these cells. The in vivo effects of diltiazem on GCase were evaluated in mice homozygous wild-type (WT), V394L and D409H. In D409H homozygotes diltiazem (10 mg/kg/d via drinking water or 50–200 mg/kg/d intraperitoneally) had minor effects on increasing GCase activity in brain and liver (1.2-fold). Diltiazem treatment (10 mg/kg/d) had essentially no effect on WT and V394L GCase protein or activity levels (<1.2-fold) in liver. These results show that LTCC blockers had the ex vivo effects of increasing GCase activity and protein in the mouse fibroblasts, but these effects did not translate into similar changes in vivo even at very high drug doses

Effects of alcohol preload on attentional bias towards cocaine-related cues

Background Drug and alcohol users have an ‘attentional bias’ for substance-related cues, which is likely to reflect the incentive-motivational properties of those cues. Furthermore, administration of an alcohol preload increases attentional bias for alcohol and tobacco-related cues in heavy drinkers and tobacco smokers, respectively. The present study investigated attentional bias for cocaine cues in cocaine users and non-users following administration of either alcohol or placebo. Method Thirty-two regular cocaine users and 40 non-users took part. Participants were administered alcohol or placebo, and administration was double blind. After drink administration, a Visual Probe task and Modified Stroop task were used to assess attentional bias. Subjective craving and alcohol outcome expectancies were also measured. Results There was a significant interaction between group and drink type on the visual probe task indicating that cocaine users who had received alcohol had increased attentional bias for cocaine pictures compared to non-users and cocaine users who received placebo. The cocaine Stroop revealed no differences between cocaine users and non-users, and no effects of alcohol in either group. Conclusions Alcohol preload in regular cocaine users increases attentional bias for cocaine cues. However, cocaine users who received placebo did not show attentional bias for cocaine stimuli. Future research should investigate the effects of alcohol preload on attentional bias in cocaine-dependent individuals

SJS/TEN 2019: From Science to Translation

Stevens-Johnson syndrome and toxic epidermal necrolysis (SJS/TEN) are potentially life-threatening, immune-mediated adverse reactions characterized by widespread erythema, epidermal necrosis, and detachment of skin and mucosa. Efforts to grow and develop functional international collaborations and a multidisciplinary interactive network focusing on SJS/TEN as an uncommon but high burden disease will be necessary to improve efforts in prevention, early diagnosis and improved acute and long-term management. SJS/TEN 2019: From Science to Translation was a 1.5-day scientific program held April 26-27, 2019, in Vancouver, Canada. The meeting successfully engaged clinicians, researchers, and patients and conducted many productive discussions on research and patient care needs

Identification of Brain Nuclei Implicated in Cocaine-Primed Reinstatement of Conditioned Place Preference: A Behaviour Dissociable from Sensitization

Relapse prevention represents the primary therapeutic challenge in the treatment of drug addiction. As with humans, drug-seeking behaviour can be precipitated in laboratory animals by exposure to a small dose of the drug (prime). The aim of this study was to identify brain nuclei implicated in the cocaine-primed reinstatement of a conditioned place preference (CPP). Thus, a group of mice were conditioned to cocaine, had this place preference extinguished and were then tested for primed reinstatement of the original place preference. There was no correlation between the extent of drug-seeking upon reinstatement and the extent of behavioural sensitization, the extent of original CPP or the extinction profile of mice, suggesting a dissociation of these components of addictive behaviour with a drug-primed reinstatement. Expression of the protein product of the neuronal activity marker c-fos was assessed in a number of brain regions of mice that exhibited reinstatement (R mice) versus those which did not (NR mice). Reinstatement generally conferred greater Fos expression in cortical and limbic structures previously implicated in drug-seeking behaviour, though a number of regions not typically associated with drug-seeking were also activated. In addition, positive correlations were found between neural activation of a number of brain regions and reinstatement behaviour. The most significant result was the activation of the lateral habenula and its positive correlation with reinstatement behaviour. The findings of this study question the relationship between primed reinstatement of a previously extinguished place preference for cocaine and behavioural sensitization. They also implicate activation patterns of discrete brain nuclei as differentiators between reinstating and non-reinstating mice