UDP-glucose 4-epimerase from saccharomyces fragilis. Presence of an essential arginine residue at the substrate-binding site of the enzyme

UDP-glucose 4-epimerase from Saccharomyces fragilis was inactivated by the arginine-specific reagents phenylglyoxal, 1,2-cyclohexanedione, and 2,3-butanedione following pseudo first order reaction kinetics. The reaction order with respect to phenylglyoxal was 1.8 and that with respect to the other two diones was close to unity. Protection afforded by substrate and competitive inhibitors against inactivation by phenylglyoxal and the reduced interaction of 1-anilinonaphthalene 8-sulfonic acid, a fluorescent probe for the substrate-binding region after phenylglyoxal modification, suggested the presence of an essential arginine residue at the substrate-binding region. Experiments with [7-14C]phenylglyoxal in the presence of UMP, a ligand known to interact at the substrate-binding region, showed that only the arginine residue at the active site could be modified by phenylglyoxal. The characteristic coenzyme fluorescence of the yeast enzyme was found to be enhanced three times in phenylglyoxal-inactivated enzyme suggesting the incorporation of the phenyl ring near the pyridine moiety of NAD

Chiral symmetry breaking and stability of quark droplets

We discuss the stability of strangelets -- quark droplets with strangeness -- in the Nambu--Jona-Lasinio model supplemented by a boundary condition for quark confinement. Effects of dynamical chiral symmetry breaking are considered properly inside quark droplets of arbitrary baryon number. We obtain the energy per baryon number of quark droplets with baryon number from one to thousands. It is shown that strangelets are not the ground states as compared with nuclei, though they can be locally stable

Production and detection of doubly charmed tetraquarks

The feasibility of tetraquark detection is studied. For the cc\bar{u}\bar{d} tetraquark we show that in present (SELEX, Tevatron, RHIC) and future facilities (LHCb, ALICE) the production rate is promising and we propose some detectable decay channels.Comment: 6 pages, 5 figure

Recrystallization of epitaxial GaN under indentation

We report recrystallization of epitaxial (epi-) GaN(0001) film under indentation.Hardness value is measured close to 10 GPa, using a Berkovich indenter. Pop-in burst in the loading line indicates nucleation of dislocations setting in plastic motion of lattice atoms under stress field for the recrystallization process. Micro-Raman studies are used to identify the recrystallization process. Raman area mapping indicates the crystallized region. Phonon mode corresponding to E2(high) close to 570 cm-1 in the as-grown epi-GaN is redshifted to stress free value close to 567 cm-1 in the indented region. Evolution of A1(TO) and E1(TO) phonon modes are also reported to signify the recrystallization process.Comment: 10 pages, 3 figures

On polynomial solutions of Heun equation

By making use of a recently developed method to solve linear differential equations of arbitrary order, we find a wide class of polynomial solutions to the Heun equation. We construct the series solution to the Heun equation before identifying the polynomial solutions. The Heun equation extended by the addition of a term, - \s/x, is also amenable for polynomial solutions.Comment: 4 pages, No figur

Microstructural evolution in the intercritical heat affected zone of a boron containing modified 9Cr-1Mo steel

Type IV cracking observed in high Cr ferritic steels is attributed to poor creep properties of the intercritical heat affected zone (ICHAZ) of the weld joint which in turn associated with the partial transformation that take place in this zone during the weld thermal cycle and resulting refinement of the microstructure. Recent studies on steels with controlled addition of boron has shown that creep strength of ICHAZ in these steels are comparable to that of the base metal and the microstructure of this zone is significantly different from that of the steels with out boron. Hence in this paper microstructural evolution in the simulated ICHAZ of two different P91 steels, one without boron and another with boron and reduced nitrogen content has been studied. Results indicate that during heating part of the weld thermal cycle, austenite nucleate and grow into two different morphologies, globular and acicular which transform to martensite during cooling. The former is more prevalent along the prior austenite grain boundaries (PAGB) of the base metal while the latter along the lath boundaries of the tempered martensite within the grains. Results also show the transformation to austenite is delayed in the boron containing steels and austenite with both the morphologies are formed. However, growth of the globular austenite, formed along the PAGB is sluggish and hence PAGBs are discernable even after the transformation. This explains the difference in the microstructure of the of ICHAZ of steels with boron and without boron and this could also be the reason for the improved creep resistance of the weld joint of high Cr ferritic steels with controlled addition of boron

Study of the leptonic decays of pseudoscalar B,DB, D and vector B∗,D∗B^*, D^* mesons and of the semileptonic B→DB\to D and B→D∗B\to D^* decays

We present results for different observables in weak decays of pseudoscalar and vector mesons with a heavy $c$ or $b$ quark. The calculations are done in a nonrelativistic constituent quark model improved at some instances by heavy quark effective theory constraints. We determine pseudoscalar and vector meson decay constants that within a few per cent satisfy $f_V M_V/f_P M_P=1$, a result expected in heavy quark symmetry when the heavy quark masses tend to infinity. We also analyze the semileptonic $B\to D$ and $B\to D^*$ decays for which we evaluate the different form factors. Here we impose heavy quark effective theory constraints among form factors that are not satisfied by a direct quark model calculation. The value of the form factors at zero recoil allows us to determine, by comparison with experimental data, the value of the $|V_{cb}|$ Cabbibo-Kobayashi-Maskawa matrix element. From the $B\to D$ semileptonic decay we get $|V_{cb}|=0.040\pm0.006$ in perfect agreement with our previous determination based on the study of the semileptonic $\Lambda_b\to \Lambda_c$ decay and also in excellent agreement with a recent experimental determination by the DELPHI Collaboration. We further make use of the partial conservation of axial current hypothesis to determine the strong coupling constants $g_{B^*B\pi}(0)=60.5\pm 1.1$ and $g_{D^*D\pi}(0)=22.1\pm0.4$. The ratio $R=(g_{B^*B\pi}(0) f_{B^*}\sqrt{M_D})/ (g_{D^*D\pi}(0) f_{D^*}\sqrt{M_B})=1.105\pm0.005$ agrees with the heavy quark symmetry prediction of 1.Comment: 19 Latex pages,6 figures, references added, corrected typos, content enlarge

A semiclassical analysis of the Efimov energy spectrum in the unitary limit

We demonstrate that the (s-wave) geometric spectrum of the Efimov energy levels in the unitary limit is generated by the radial motion of a primitive periodic orbit (and its harmonics) of the corresponding classical system. The action of the primitive orbit depends logarithmically on the energy. It is shown to be consistent with an inverse-squared radial potential with a lower cut-off radius. The lowest-order WKB quantization, including the Langer correction, is shown to reproduce the geometric scaling of the energy spectrum. The (WKB) mean-squared radii of the Efimov states scale geometrically like the inverse of their energies. The WKB wavefunctions, regularized near the classical turning point by Langer's generalized connection formula, are practically indistinguishable from the exact wave functions even for the lowest ($n=0$) state, apart from a tiny shift of its zeros that remains constant for large $n$.Comment: LaTeX (revtex 4), 18pp., 4 Figs., already published in Phys. Rev. A but here a note with a new referece is added on p. 1