ATP4 and ciliation in the neuroectoderm and endoderm of Xenopus embryos and tadpoles

AbstractDuring gastrulation and neurulation, foxj1 expression requires ATP4a-dependent Wnt/β-catenin signaling for ciliation of the gastrocoel roof plate (Walentek et al. Cell Rep. 1 (2012) 516–527.) and the mucociliary epidermis (Walentek et al. Dev. Biol. (2015)) of Xenopus laevis embryos. These data suggested that ATP4a and Wnt/β-catenin signaling regulate foxj1 throughout Xenopus development. Here we analyzed whether foxj1 expression was also ATP4a-dependent in other ciliated tissues of the developing Xenopus embryo and tadpole. We found that in the floor plate of the neural tube ATP4a-dependent canonical Wnt signaling was required for foxj1 expression, downstream of or in parallel to Hedgehog signaling. In the developing tadpole brain, ATP4-function was a prerequisite for the establishment of cerebrospinal fluid flow. Furthermore, we describe foxj1 expression and the presence of multiciliated cells in the developing tadpole gastrointestinal tract. Our work argues for a general requirement of ATP4-dependent Wnt/β-catenin signaling for foxj1 expression and motile ciliogenesis throughout Xenopus development

A protocol for the development of Core Outcome Sets for effectiveness trials and clinical audits in Renal Cell Cancer (R-COS)

The data collection for the interview study is funded by NHS Grampian Endowments, and the costs of the interview transcriptions and eDelphi licences will be paid by the Arcobaleno Cancer Trust. Neither funder had any role in the design of the study. All other parts of the study are currently unfunded. The research team is not personally reimbursed for their time and efforts apart from research input by SD, which is financed by Swedish government funding of clinical research (ALF).Peer reviewedPublisher PD