24 research outputs found

    Elemental and chemically specific x-ray fluorescence imaging of biological systems

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    Materials in the field: object-trajectories and object-positions in the field of contemporary art

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    The paper explores the centrality of the physical art object in the field of contemporary art with reference to their material specificities. It is based on an ethnographic study of the conservation laboratory at the Museum of Modern Art (MoMA) in New York and draws from three detailed case-studies where the temporal and spatial trajectory of art objects led to changes in the processes of collaboration, competition, and repositioning of those agents involved in production, exhibition and conservation. The study demonstrates the importance of artworks qua physical objects in the field of contemporary art, claiming attention to materiality in field theory and developing an object-orientation methodology in field analysis. Artworks are shown to intervene in field processes both reproducing divisions and re-drawing boundaries within and between fields, actualizing positions of individuals and institutions

    Elemental characterisation of the pyramidal neuron layer within the rat and mouse hippocampus.

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    A unique combination of sensitivity, resolution, and penetration make X-ray fluorescence imaging (XFI) ideally suited to investigate trace elemental distributions in the biological context. XFI has gained widespread use as an analytical technique in the biological sciences, and in particular enables exciting new avenues of research in the field of neuroscience. In this study, elemental mapping by XFI was applied to characterise the elemental content within neuronal cell layers of hippocampal sub-regions of mice and rats. Although classical histochemical methods for metal detection exist, such approaches are typically limited to qualitative analysis. Specifically, histochemical methods are not uniformly sensitive to all chemical forms of a metal, often displaying variable sensitivity to specific "pools" or chemical forms of a metal. In addition, histochemical methods require fixation and extensive chemical treatment of samples, creating the strong likelihood for metal redistribution, leaching, or contamination. Direct quantitative elemental mapping of total elemental pools, in situ within ex vivo tissue sections, without the need for chemical fixation or addition of staining reagents is not possible with traditional histochemical methods; however, such a capability, which is provided by XFI, can offer an enormous analytical advantage. The results we report herein demonstrate the analytical advantage of XFI elemental mapping for direct, label-free metal quantification, in situ within ex vivo brain tissue sections. Specifically, we definitively characterise for the first time, the abundance of Fe within the pyramidal cell layers of the hippocampus. Localisation of Fe to this cell layer is not reproducibly achieved with classical Perls histochemical Fe stains. The ability of XFI to directly quantify neuronal elemental (P, S, Cl, K, Ca, Fe, Cu, Zn) distributions, revealed unique profiles of Fe and Zn within anatomical sub-regions of the hippocampus i.e., cornu ammonis 1, 2 or 3 (CA1, CA2 or CA3) sub-regions. Interestingly, our study reveals a unique Fe gradient across neuron populations within the non-degenerating and pathology free rat hippocampus, which curiously mirrors the pattern of region-specific vulnerability of the hippocampus that has previously been established to occur in various neurodegenerative diseases
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