Diversity, Distribution and Biological Activity of Soft Corals (Octocorallia, Alcyonacea) in Singapore

The Southern Islands of Singapore are known to contain coral reefs which are high in biodiversity. However, the diversity of soft corals had received little attention to date. This study was conducted to determine the soft coral diversity in Singapore reefs as well as to conduct preliminary bioactivity tests on the organic extracts from these soft corals. A 100-meter line transect was used to survey soft corals at a 3m depth at ten different sample sites. Sclerites from samples were used to identify the soft corals to the generic level. This study uncovered the following genera of soft corals: Carijoa spp., Cladiella spp., Sinularia spp., Lobophytum spp., Sarcophyton spp., Stereonephthya spp., and Nephthea spp. In addition, an unidentified genus of soft coral was observed at Kusu Island. Cladiella spp. yielded the highest number of colonies, and Sarcophyton spp. had the highest coverage in terms of total colony diameter. The brine shrimp (Artemia salina) toxicity assay was carried out to screen for toxicity of the soft coral extracts at concentrations of 10, 100, and 1000 ppm. Results showed high levels of toxicity in extracts of Sarcophyton spp. and Cladiella spp., indicating that these soft corals are potentially good sources of bioactive compounds for drug discovery

Chandra Observations of the Interacting NGC 4410 Galaxy Group

We present high resolution X-ray imaging data from the ACIS-S instrument on the Chandra telescope of the nearby interacting galaxy group NGC 4410. Four galaxies in the inner portion of this group are clearly detected by Chandra, including the peculiar low luminosity radio galaxy NGC 4410A. In addition to a nuclear point source, NGC 4410A contains diffuse X-ray emission, including an X-ray ridge extending out to about 12" (6 kpc) to the northwest of the nucleus. This ridge is coincident with an arc of optical emission-line gas, which has previously been shown to have optical line ratios consistent with shock ionization. This structure may be due to an expanding superbubble of hot gas caused by supernovae and stellar winds or by the active nucleus. The Chandra observations also show four or five possible compact ultra-luminous X-ray (ULX) sources (L(x) >= 10^39 erg/s) associated with NGC 4410A. At least one of these candidate ULXs appears to have a radio counterpart, suggesting that it may be due to an X-ray binary with a stellar-mass black hole, rather than an intermediate mass black hole. In addition, a faint diffuse intragroup X-ray component has been detected between the galaxies (L(x) ~ 10^41 erg/s). This supports the hypothesis that the NGC 4410 group is in the process of evolving via mergers from a spiral-dominated group (which typically have no X-ray-emitting intragroup gas) to an elliptical-dominated group (which often have a substantial intragroup medium).Comment: 27 pages, 14 figures; Accepted by Astronomical Journal; color images at http://www.etsu.edu/physics/bsmith/research/n4410.htm

Atomic Hydrogen and Star Formation in the Bridge/Ring Interacting Galaxy Pair NGC 7714/7715 (Arp 284)

We present high spatial resolution 21 cm HI maps of the interacting galaxy pair NGC 7714/7715. We detect a massive (2 x 10**9 M(sun)) HI bridge connecting the galaxies that is parallel to but offset from the stellar bridge. A chain of HII regions traces the gaseous bridge, with H-alpha peaks near but not on the HI maxima. An HI tidal tail is also detected to the east of the smaller galaxy NGC 7715, similarly offset from a stellar tail. The strong partial stellar ring on the eastern side of NGC 7714 has no HI counterpart, but on the opposite side of NGC 7714 there is a 10**9 M(sun) HI loop 11 kpc in radius. Within the NGC 7714 disk, clumpy HI gas is observed associated with star formation regions. Redshifted HI absorption is detected towards the starburst nucleus. We compare the observed morphology and gas kinematics with gas dynamical models in which a low-mass companion has an off-center prograde collision with the outer disk of a larger galaxy. These simulations suggest that the bridge in NGC 7714/7715 is a hybrid between bridges seen in systems like M51 and the purely gaseous `splash' bridges found in ring galaxies like the Cartwheel. The offset between the stars and gas in the bridge may be due to dissipative cloud-cloud collisions occuring during the impact of the two gaseous disks.Comment: 31 pages, Latex, 11 figures, to be published in the July 10, 1997 issue of the Astrophysical Journa

New Algorithm for Mixmaster Dynamics

We present a new numerical algorithm for evolving the Mixmaster spacetimes. By using symplectic integration techniques to take advantage of the exact Taub solution for the scattering between asymptotic Kasner regimes, we evolve these spacetimes with higher accuracy using much larger time steps than previously possible. The longer Mixmaster evolution thus allowed enables detailed comparison with the Belinskii, Khalatnikov, Lifshitz (BKL) approximate Mixmaster dynamics. In particular, we show that errors between the BKL prediction and the measured parameters early in the simulation can be eliminated by relaxing the BKL assumptions to yield an improved map. The improved map has different predictions for vacuum Bianchi Type IX and magnetic Bianchi Type VI$_0$ Mixmaster models which are clearly matched in the simulation.Comment: 12 pages, Revtex, 4 eps figure

Structural basis for complement factor H-linked age-related macular degeneration

This is the final version of the article. Available from the publisher via the DOI in this record.Nearly 50 million people worldwide suffer from age-related macular degeneration (AMD), which causes severe loss of central vision. A single-nucleotide polymorphism in the gene for the complement regulator factor H (FH), which causes a Tyr-to-His substitution at position 402, is linked to approximately 50% of attributable risks for AMD. We present the crystal structure of the region of FH containing the polymorphic amino acid His402 in complex with an analogue of the glycosaminoglycans (GAGs) that localize the complement regulator on the cell surface. The structure demonstrates direct coordination of ligand by the disease-associated polymorphic residue, providing a molecular explanation of the genetic observation. This glycan-binding site occupies the center of an extended interaction groove on the regulator's surface, implying multivalent binding of sulfated GAGs. This finding is confirmed by structure-based site-directed mutagenesis, nuclear magnetic resonance-monitored binding experiments performed for both H402 and Y402 variants with this and another model GAG, and analysis of an extended GAG-FH complex.B. Prosser is funded by the Wellcome Trust Structural Biology Training Program (075415/Z/04/Z). S. Johnson and P. Roversi were funded by grants to S.M. Lea from the Medical Research Council (MRC) of the United Kingdom (grants G0400389 and G0400775). D. Uhrin and P.N. Barlow were funded by the Wellcome Trust (078780/ Z/05/Z). S.J. Clark was funded by an MRC Doctoral Training Account (G78/7925), and R.B. Sim and A.J. Day were funded by MRC core funding to the MRC Immunochemistry Unit

Mixmaster Behavior in Inhomogeneous Cosmological Spacetimes

Numerical investigation of a class of inhomogeneous cosmological spacetimes shows evidence that at a generic point in space the evolution toward the initial singularity is asymptotically that of a spatially homogeneous spacetime with Mixmaster behavior. This supports a long-standing conjecture due to Belinskii et al. on the nature of the generic singularity in Einstein's equations.Comment: 4 pages plus 4 figures. A sentence has been deleted. Accepted for publication in PR

Direct comparison between confocal and multiphoton microscopy for rapid histopathological evaluation of unfixed human breast tissue

Rapid histopathological examination of surgical specimen margins using fluorescence microscopy during breast conservation therapy has the potential to reduce the rate of positive margins on postoperative histopathology and the need for repeat surgeries. To assess the suitability of imaging modalities, we perform a direct comparison between confocal fluorescence microscopy and multiphoton microscopy for imaging unfixed tissue and compare to paraffin-embedded histology. An imaging protocol including dual channel detection of two contrast agents to implement virtual hematoxylin and eosin images is introduced that provides high quality imaging under both one and two photon excitation. Corresponding images of unfixed human breast tissue show that both confocal and multiphoton microscopy can reproduce the appearance of conventional histology without the need for physical sectioning. We further compare normal breast tissue and invasive cancer specimens imaged at multiple magnifications, and assess the effects of photobleaching for both modalities using the staining protocol. The results demonstrate that confocal fluorescence microscopy is a promising and cost-effective alternative to multiphoton microscopy for rapid histopathological evaluation of ex vivo breast tissue.National Institutes of Health (U.S.) (Grant R01-CA178636-02)National Institutes of Health (U.S.) (Grant R01-CA075289-18)National Institutes of Health (U.S.) (Grant F32-CA183400-03)United States. Air Force. Office of Scientific Research (Grant FA9550-12-1-0551)United States. Air Force. Office of Scientific Research (Grant FA9550-15-1-0473