Fall Prevention in Older Adults: Evidence and Key Indicators in the Promotion of Fall Prevention

This video presentation covers risk factors important to fall prevention in older adults. In addition to discussing the etiology of falls, the presentation outlines evidence-based assessments and treatments. Finally it briefly addresses the role of interdisciplinary team members in the prevention of falls

Preparing for a Short-Term Volunteer Experience

Occupational Therapy (OT) practitioners who volunteer for short-term medical service trips in developing countries face many challenges, including gaining an appreciation of that country\u27s culture (values, economy, political, and medical systems) and understanding how these factors influence provision of OT services. As OT practitioners, we contribute to the global health of society and individuals by enabling the right to engage in meaningful, purposeful occupations (World Federation of Occupational Therapists, n.d., para 4). The frequency of short-term medical service trips (STMST) undertaken by persons living in high income countries to low income countries is increasing (Sykes, 2014). Despite this fact, systematic research identifying best practices for such trips is limited to low-level evidence studies. Although the amount of OT literature extolling culturally-sensitive practices has grown (Began, 2015), most articles are directed toward first-world rather than third-world countries (like Haiti\u27s). These limitations handicap therapists who seek knowledge of how to provide occupation-based interventions in a developing country (Compton, 2013). This session will present an overview of global health initiatives involving OT, summarize available research about STMST, and identify key aspects of culture and diversity which need to be considered by therapists providing STMST experiences. A case study of service trips to Haiti will be used to demonstrate the application of research-based recommendations to clinical practice. Small group discussions will encourage self-reflection of cultural beliefs impacting the provision of international treatment. Resources and recommendations will be shared to assist therapists in planning short-term service trips that are culturally sensitive and collaborative

Centromere identity in Drosophila is not determined in vivo by replication timing

Centromeric chromatin is uniquely marked by the centromere-specific histone CENP-A. For assembly of CENP-A into nucleosomes to occur without competition from H3 deposition, it was proposed that centromeres are among the first or last sequences to be replicated. In this study, centromere replication in Drosophila was studied in cell lines and in larval tissues that contain minichromosomes that have structurally defined centromeres. Two different nucleotide incorporation methods were used to evaluate replication timing of chromatin containing CID, a Drosophila homologue of CENP-A. Centromeres in Drosophila cell lines were replicated throughout S phase but primarily in mid S phase. However, endogenous centromeres and X-derived minichromosome centromeres in vivo were replicated asynchronously in mid to late S phase. Minichromosomes with structurally intact centromeres were replicated in late S phase, and those in which centric and surrounding heterochromatin were partially or fully deleted were replicated earlier in mid S phase. We provide the first in vivo evidence that centromeric chromatin is replicated at different times in S phase. These studies indicate that incorporation of CID/CENP-A into newly duplicated centromeres is independent of replication timing and argue against determination of centromere identity by temporal sequestration of centromeric chromatin replication relative to bulk genomic chromatin

Variation in the CENP-A sequence association landscape across diverse inbred mouse strains.

Centromeres are crucial for chromosome segregation, but their underlying sequences evolve rapidly, imposing strong selection for compensatory changes in centromere-associated kinetochore proteins to assure the stability of genome transmission. While this co-evolution is well documented between species, it remains unknown whether population-level centromere diversity leads to functional differences in kinetochore protein association. Mice (Mus musculus) exhibit remarkable variation in centromere size and sequence, but the amino acid sequence of the kinetochore protein CENP-A is conserved. Here, we apply k-mer-based analyses to CENP-A chromatin profiling data from diverse inbred mouse strains to investigate the interplay between centromere variation and kinetochore protein sequence association. We show that centromere sequence diversity is associated with strain-level differences in both CENP-A positioning and sequence preference along the mouse core centromere satellite. Our findings reveal intraspecies sequence-dependent differences in CENP-A/centromere association and open additional perspectives for understanding centromere-mediated variation in genome stability

Reaching for the Cap and Gown: Progress Toward Success Boston's College Completion Goals for Graduates of the Boston Public Schools

A new report, prepared for Mayor Martin J. Walsh and the Success Boston college completion initiative, shows a remarkable increase in both the percentage and the number of Boston Public Schools graduates who complete college within six years. The report also examines college completion for students with Success Boston coaches, a major intervention launched by the Boston Foundation and its partners, including the Boston Public Schools, in 2009. Success Boston, a citywide multi-sector college completion initiative, was launched in 2008 in response to a report that found that only 35% of the BPS Class of 2000 graduates who enrolled in college earned a degree within seven years of graduating high school. The initiative is guided by the Boston Public Schools, the Boston Foundation, UMass Boston, Bunker Hill Community College, and the Boston Private Industry Council, along with dozens of colleges, universities, and nonprofit organizations. Among the initiative's ambitious goals was pushing members of the BPS Class of 2009 to a 52%six-year college completion rate. Today's report, "Reaching for the Cap and Gown: Progress Toward Success Boston's College Completion Goals for Graduates of the Boston Public Schools," finds that the six-year college completion rate of first-year college enrollees from the BPS Class of 2009 was 51.3%--within one percentage point of the 52% goal set in 2008. Equally impressive is the gain in the number of BPS graduates completing college within six years of high school graduation--1,314 from the Class of 2009, compared to 735 from the Class of 2000, the equivalent of a 79% increase. The study also finds that college completion, at 54.7%, is even higher than the goal for students who enrolled in the fall immediately after graduating from high school

MU2 and HP1a Regulate the Recognition of Double Strand Breaks in Drosophila melanogaster

Chromatin structure regulates the dynamics of the recognition and repair of DNA double strand breaks; open chromatin enhances the recruitment of DNA damage response factors, while compact chromatin is refractory to the assembly of radiation-induced repair foci. MU2, an orthologue of human MDC1, a scaffold for ionizing radiation-induced repair foci, is a widely distributed chromosomal protein in Drosophila melanogaster that moves to DNA repair foci after irradiation. Here we show using yeast 2 hybrid screens and co-immunoprecipitation that MU2 binds the chromoshadow domain of the heterochromatin protein HP1 in untreated cells. We asked what role HP1 plays in the formation of repair foci and cell cycle control in response to DNA damage. After irradiation repair foci form in heterochromatin but are shunted to the edge of heterochromatic regions an HP1-dependent manner, suggesting compartmentalized repair. Hydroxyurea-induced repair foci that form at collapsed replication forks, however, remain in the heterochromatic compartment. HP1a depletion in irradiated imaginal disc cells increases apoptosis and disrupts G2/M arrest. Further, cells irradiated in mitosis produced more and brighter repair foci than to cells irradiated during interphase. Thus, the interplay between MU2 and HP1a is dynamic and may be different in euchromatin and heterochromatin during DNA break recognition and repair

Dedication to Prof. T. Suzuki

『千葉大学法学論集』第28巻第1・2号 半田吉信先生・鈴木庸夫先生 退職記念号HOGAKU RONSHU(CHIBA JOURNAL OF LAW AND POLITICS) Vol.28 Nos.1・2 Essays in Honor of Professors Yoshinobu Handa and Tsuneo Suzuki at their Retirement from Chiba Universit

Numerical Analysis of Etoposide Induced DNA Breaks

Background: Etoposide is a cancer drug that induces strand breaks in cellular DNA by inhibiting topoisomerase II (topoII) religation of cleaved DNA molecules. Although DNA cleavage by topoisomerase II always produces topoisomerase II-linked DNA double-strand breaks (DSBs), the action of etoposide also results in single-strand breaks (SSBs), since religation of the two strands are independently inhibited by etoposide. In addition, recent studies indicate that topoisomerase II-linked DSBs remain undetected unless topoisomerase II is removed to produce free DSBs. Methodology/Principal Findings: To examine etoposide-induced DNA damage in more detail we compared the relative amount of SSBs and DSBs, survival and H2AX phosphorylation in cells treated with etoposide or calicheamicin, a drug that produces free DSBs and SSBs. With this combination of methods we found that only 3 % of the DNA strand breaks induced by etoposide were DSBs. By comparing the level of DSBs, H2AX phosphorylation and toxicity induced by etoposide and calicheamicin, we found that only 10 % of etoposide-induced DSBs resulted in histone H2AX phosphorylation and toxicity. There was a close match between toxicity and histone H2AX phosphorylation for calicheamicin and etoposide suggesting that the few etoposide-induced DSBs that activated H2AX phosphorylation were responsible for toxicity. Conclusions/Significance: These results show that only 0.3 % of all strand breaks produced by etoposide activate H2A

Secure care pathway and standards : co-production process and implementation plans

The development of the Secure Care Pathway and Standards sets out, for the first time, national standards of what support children and young people in Scotland should expect when in, or on the edges of, secure care. Launched in October 2020, the Standards seek to ensure support is provided before, during and after a stay in secure care and that the rights of children and young people, often facing extreme vulnerabilities and risks in their lives, are respected. When implemented, the Standards will deliver a consistent, unified approach to caring for vulnerable children in all council areas, and to all children placed in secure care in Scotland. Crucially, the Standards were developed using co-production methods alongside children and young people. This article describes some of the elements of that process, as well as the impact that the new Standards will have on young people's lives in Scotland