『千葉大学法学論集』第28巻第1・2号 半田吉信先生・鈴木庸夫先生 退職記念号HOGAKU RONSHU(CHIBA JOURNAL OF LAW AND POLITICS) Vol.28 Nos.1・2 Essays in Honor of Professors Yoshinobu Handa and Tsuneo Suzuki at their Retirement from Chiba Universit

Beth Sullivan (3578426)

Justyne Ross (3578429)

Kaitlin Woodlief (3578432)

Additional file 5: Figure S3. Experimental strategy for isolation of G1, G1/S, and S phase chromatin fibers. (a) G1 (top), G1/S (middle), S (bottom) phase synchronization scheme for LCL and fibroblast lines using nocodazole block and release, serum starvation, and/or double thymidine blocks. The thymidine analog EdU was used in each experiment to differentiate between unreplicated and replicated centromeres. (b) Representative chromatin fibers stained with DAPI, EdU, and CENP-A antibodies (not shown), showing that the synchronization schemes were successful. Only EdU-negative fibers were analyzed for G1 and G1/S experiments; only EdU-positive fibers were analyzed for S phase experiments

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MOESM5 of Inheritance of the CENP-A chromatin domain is spatially and temporally constrained at human centromeres

http://opac.ll.chiba-u.jp/da/curator/900117215/09127208_28-1_5.pdf

Dedication to Prof. T. Suzuki

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