DETERMINATION OF DEVELOPMENTAL AND LIFE TABLE PARAMETERS OF RHYZOBIUS LOPHANTHAE BLAISDELL (COLEOPTERA COCCINELLIDAE) ON THREE ARMORED SCALE INSECTS (HEMIPTERA DIASPIDIDAE)

Scale insects are the most damaging pests of citrus production in Turkey. Rhyzobius lophanthae Blaisdell (Coleoptera: Coccinellidae), a polyphagous coccinellid, is one of the most important predators of these pests. In this study, the life tables of R. lophanthae on three different armored scale insects, Aonidiella aurantii (Maskell), Aspidiotus nerii Bouché, Chrysomphalus dictiyospermi (Morgan) (Hemiptera: Diaspididae), were produced. The study was conducted in a climate chamber under constant conditions of 26°C, 60% relative humidity and 16-hour photoperiod.The net reproductive rate (R0), intrinsic rate of increase (rm) and mean generation time (T0) were 36.027, 12.520 and 6.600 females/female/generation, 0.120, 0.061 and 0.041 females/female/day, and 30.005, 41.151, 45.826 days, respectively, when R. lophanthae was reared on A. nerii, C. dictiyospermi and A. aurantii, respectively. The doubling time (DT) and finite rates of increase (λ) were 5.803, 11.286 and 16.832 days, 1.127, 1.063 and 1.042 individuals/female/day, respectively, when R. lophanthae was reared on A. nerii, C. dictiyospermi and A. aurantii, respectively

A rarely seen breast tumor: looking over the literature related to intracystic papillary carcinoma

Intracystic papillary carcinoma of the breast is a rarely seen tumor. It is often classified as a variant of in situ ductal carcinoma. Intracystic papillary carcinoma is slow growing and usually surrounded and limited by fibrosis capsule. Presently described is case of a 51-year-old postmenopausal patient. She felt a tumor in her left breast, and after examination, it was diagnosed as intracystic papillary carcinoma. Following adjuvant chemotherapy breast excision, and axillar dissection, patient was given curative external radiotherapy and hormonal treatment. As result of 40-month follow-up, the patient was evaluated as having local recurrence and no distant metastases. Follow-up and treatment protocols should be observed on case-by-case basi

In vitro antimicrobial and anti-mycobacterial activity of Piper nigrum Linn. essential oil

In the present study, it was aimed to determine the phytochemical components and evaluate the antimicrobial and antimycobacterial activity of Piper nigrum L. (Piperaceae) essential oil. It is well known that black pepper preparations have a wide spectrum of biological activity along with its antimicrobial activity. The antimicrobial properties of black pepper extracts were evaluated previously [1,2].                Commercially available black pepper dried fruits were used as study material. The dry black pepper fruits were pounded, and the essential oil was obtained by hydrodistillation using the Clevenger apparatus. The phytochemical analysis of the essential oil was performed by GC-FID and GC/MS, simultaneously. In addition, in vitro antibacterial and antimycobacterial effects against Staphylococcus aureus, S. epidermidis, methicillin-resistant S. aureus, Streptococcus pyogenes, Moraxella catarrhalis, Haemophilus influenza, Mycobacterium smegmatis, M. avium and M. fortuitum subsp. fortuitum were assessed by microdilution methods according to CLSI standards [3,4].                The black pepper essential oil was obtained in 10.7 mL/kg yield. According to the GC/FID and GC/MS results, the major constituent of the oil was determined as caryophyllene oxide (28.7%). The antibacterial activity results showed that the oil was effective at different concentrations in the concentration range of 0.16-2.5 mg/mL. According to the antimycobacterial activity results, the essential oil’s minimum inhibitory concentrations were 0.08-0.31 mg/mL. LOX-antiinflammatory activity and Caenorhabditis elegans toxicity evaluations are under progress for the mode of action and selectivity of the activity

Intraoral premalign ve malign lezyonlu hastaların doku örneklerindeki candida türlerinin DNA analiziyle belirlenmesi ve sağlıklı doku ile karşılaştırılması

Amaç: Bu retrospektif çalışmada premalign ve malign lezyonlu ile sağlıklı bireylere ait doku örneklerindeki Candida albicans, Candida kruseii, Candida parapsilosis, Candida tropicalis ve Candida glabrata türlerinin belirlenmesi amaçlanmıştır.  Gereç ve Yöntem: Çalışmaya oral premalign veya malign lezyon şüphesi nedeniyle biyopsi uygulanmış doku örnekleri dahil edildi. Örnekler histolojik özelliklerine göre sağlıklı (n=20), displazi (n=20), karsinoma in-situ (n=20) ve skuamoz hücreli karsinom (SHK) (n=20) olmak üzere 4 gruba ayrıldı. DNA izolasyonunun ardından Candida türlerine ait DNA örneklerinin miktar ve saflık kontrolleri gerçekleştirildi. Kantitatif ölçümler için Real-Time PCR (LightCycler 96, Almanya) kullanıldı ve standart eğriler hesaplandı. Veriler Mann-Whitney ve Wilcoxon Signed Ranks testleri ile değerlendirildi (P<0.05).Bulgular: Sağlıklı ve displazik örneklerin yer aldığı gruplarda C. parapsilosis (104DNA/50 mg) en sık görülen Candida türüydü. Karsinoma in-situ grubunda C. tropicalis ve C. kruseii (103DNA/50 mg), SHK grubunda ise C. kruseii (104DNA/50 mg) seviyelerinin daha yüksek olduğu belirlendi. Örneklerin hiçbirinde C. albicans varlığı gösterilemedi. Gruplar arasında farklı Candida türlerinin sayısına göre anlamlı bir fark gözlenmedi (P>0.05). Farklı Candida türlerinin gruplar içerisindeki seviyeleri incelendiğinde; sağlıklı örneklerde C. glabrata (103DNA/50 mg), displazi grubunda C. parapsilosis (104DNA/50 mg), karsinoma in-situ grubunda C. tropicalis-C. kruseii (103DNA/50 mg), SHK grubunda ise C. kruseii’nin (103DNA/50 mg) en fazla sayıda gözlenen Candida türü olduğu belirlendi. Candida türlerinin gruplar içerisindeki dağılımı anlamlı değildi (P>0.05).Sonuç: Sağlıklı, displazik, karsinoma in situ ve skuamöz hücreli karsinoma doku örneklerinde Real-Time PCR yöntemiyle yapılan kantitatif değerlendirmede Candida türlerinin tüm gruplarda benzer oranda mevcut oldukları belirlendi.ANAHTAR KELİMELER Oral Candida, C. albicans, ağız kanseri, Real-Time PCR, moleküler analiz &nbsp

Recent Advances in Health Biotechnology During Pandemic

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in 2019, cut the epoch that will make profound fluctuates in the history of the world in social, economic, and scientific fields. Urgent needs in public health have brought with them innovative approaches, including diagnosis, prevention, and treatment. To exceed the coronavirus disease 2019 (COVID-19) pandemic, various scientific authorities in the world have procreated advances in real time polymerase chain reaction (RT-PCR) based diagnostic tests, rapid diagnostic kits, the development of vaccines for immunization, and the purposing pharmaceuticals for treatment. Diagnosis, treatment, and immunization approaches put for- ward by scientific communities are cross-fed from the accrued knowledge of multidisciplinary sciences in health biotechnology. So much so that the pandemic, urgently prioritized in the world, is not only viral infections but also has been the pulsion in the development of novel approaches in many fields such as diagnosis, treatment, translational medicine, virology, mi- crobiology, immunology, functional nano- and bio-materials, bioinformatics, molecular biol- ogy, genetics, tissue engineering, biomedical devices, and artificial intelligence technologies. In this review, the effects of the COVID-19 pandemic on the development of various scientific areas of health biotechnology are discussed

Effects of some entomopathogenic fungi on Citrus Mealybug Planococcus citri (Risso) (Hemiptera: Pseudococcidae)

Citrus Mealybug Planococcus citri is an economically important pest species causing harm on citrus and some other plants. Biological control of the pest is gaining importance because of the problems caused by chemical control. In this study, effects of Beauveria bassiana, Paecilomyces fumosoroseus and Paecilomyces lilacinus against second instar larvae of mealybug were investigated. Healthy potato tubers were dipped into spore suspensions of the entomopathogens with 108 conidia/ml concentration and then infested with mealybugs. Potatoes were then kept in a climatic room with 25±1°C temperature and 60±5% humidity. Alive and dead mealybug individuals were determined 3, 5 and 7 days after applications and mortality rates were calculated. As a result, three entomopathogens caused different rates of mortality on the pest. B. bassiana isolate showed the highest effect and killed all pest individuals 5 days after the application. Mortality rates caused by P. fumosoroseus and P. lilacinus were lower in the first two observations, while they showed 96% and 82% mortality on the seventh day, respectively

Bazı Uçucu Yağların <i>Myzus persicae</i> ve Avcısı <i>Chrysoperla carnea</i> Üzerindeki Etkileri

Bu çalışmada, laboratuvar koşullarında farklı bitkisel uçucu yağların Şeftali yeşil yaprakbiti Myzus persicae (Sulz.) (Hemiptera: Aphididae) ve bu zararlının avcısı olan Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) üzerindeki etkisi belirlenmiştir. Denemeler 25±1° C sıcaklığa, %60±5 ve 16:8 aydınlatma koşullarına sahip iklim odalarında gerçekleştirilmiştir. Yaprak biti bireyleri biber bitkileri (Capsicum annuum L.) üzerinde üretilirken, avcılar bu afit ile beslenmiştir.  Bitkisel yağların etkileri püskürtme yöntemi ile araştırılmıştır. Kontrol uygulamalarında saf su kullanılmıştır. Uygulamadan 1., 3., 5. ve 7. günden sonra ölü ve canlı bireyler sayılmıştır. Bitkisel yağların etkisi Abbott formülü kullanılarak değerlendirilmiştir. Denemeler 5 tekerrürlü olarak yürütülmüştür. Elde edilen sonuçlara göre; karanfil, lavanta ve ardıcın M. persicae üzerindeki 7. gündeki etkisi sırasıyla %78.26, %69.57 ve %47.83 olarak bulunmuştur. Uygulanan tüm uçucu yağların C. carnea için zararsız olduğu görülmüştür.</p

Predictive risk factors in the treatment of gestational diabetes mellitus

WOS: 000215780200004PubMed ID: 26508897OBJECTIVE: This study aims to investigate predictive risk factors in the treatment of gestational diabetes mellitus (GDM). PATIENTS AND METHODS: A total of 256 pregnant tvomen who underwent 75 g oral glucose tolerance test (OGTT) during 24-28 weeks of pregnancy were included according to the World Health Organization criteria. Demographic characteristics of the patients, including age, parity, family' history of diabetes, body weight before pregnancy, and body weight at the diagnosis of GDM, were recorded. Fasting insulin and hemoglobin Ale (HbAlc) values at the time of diagnosis were evaluated. The patients were divided into two groups: those requiring insulin treatment (insulin group, n =89) and those receiving diet therapy (diet group, 71=167) during pregnancy according to the American Diabetes Association recommend ations. RESULTS: A total of 34.760 of the pregnant women with (TDM required insulin treatment. The mean age of these patients was significantly higher compared to the diet group (34.9 +/- 0.6 years vs. 31.9 0.6 years; PS 0.004). Body mass index before pregnancy was also significantly-higher in the insulin group than that in die diet group (32 0.9 kg/m(2) vs. 29 0.7 kg/m(2); P =0.004). Fasting blood glucose (FBG) during OGTT was 105.6 2.1 mg/d L and 96.7 1.1 mg/dL in the insulin group and diet group, respectively (P < 0.001). There was no significant difference in fasting plasma glucose during OGTT between the groups (P =0.069), while plasma glucose at two Flours was 161.1 +/- 6.8 mg/d L in the insulin group and 145.1 +/- 3.7 nig/dL in the diet group (P =0.027). At the dine of diagnosis, HbAlc values were significantly higher iii the insulin group compared to the diet group (5.3 0.1 vs. 4.9 0.1; P=0.001). There was no significant difference iii FBG and homeostasis model assessment -insulin resistance values between the groups (P=0.908, P=0.073). CONCLUSION: Our study results suggest that age, family history' of diabetes, body weight befivre pregnancy, FUG-, and HbAlc values are predictors for the necessity o f insulin treatment

The effects of polycystic ovary syndrome on gestational diabetes mellitus

WOS: 000374873900012PubMed ID: 26479686The aim of this study was to explore the inter-relationship between polycystic ovary syndrome and gestational diabetes mellitus, and demonstrate maternal and fetal outcomes. This was a case-control study in 1360 pregnant women who received a diagnosis of gestational diabetes mellitus between 24 and 28 weeks of gestational age. Among all diagnosed with gestational diabetes mellitus, 150 pregnant women had received a polycystic ovary syndrome, and 160 women who did not have polycystic ovary syndrome were designated as controls. The incidence of pregnancy-induced hypertension was 26.3% and 12% in the case and control groups, respectively. Preeclampsia was seen at an incidence of 12% and 6% in case and in control groups, respectively. The difference in neonatal hypoglycemia between the two groups was statistically significant, with an incidence of 17% and 5% in the case and in control groups, respectively. This study demonstrated that the presence of polycystic ovary syndrome along with gestational diabetes mellitus increases the risk of pregnancy induced hypertension by 2.4 fold, preeclampsia by 2 fold and neonatal hypoglycemia by 3.2 fold, compared to gestational diabetes mellitus alone