Expression analysis of the family of 14-3-3 proteins in zebrafish development

Animal sciencesMicrobial Biotechnolog

Human Embryonic Stem Cell Technology: Large Scale Cell Amplification and Differentiation

Embryonic stem cells (ESC) hold the promise of overcoming many diseases as potential sources of, for example, dopaminergic neural cells for Parkinson’s Disease to pancreatic islets to relieve diabetic patients of their daily insulin injections. While an embryo has the innate capacity to develop fully functional differentiated tissues; biologists are finding that it is much more complex to derive singular, pure populations of primary cells from the highly versatile ESC from this embryonic parent. Thus, a substantial investment in developing the technologies to expand and differentiate these cells is required in the next decade to move this promise into reality. In this review we document the current standard assays for characterising human ESC (hESC), the status of ‘defined’ feeder-free culture conditions for undifferentiated hESC growth, examine the quality controls that will be required to be established for monitoring their growth, review current methods for expansion and differentiation, and speculate on the possible routes of scaling up the differentiation of hESC to therapeutic quantities

Structure of molten Al and eutectic Al–Si alloy studied by neutron diffraction

International audienc

Structure of molten Al and eutectic Al–Si alloy studied by neutron diffraction

International audienc

Anelastic strain and anisotropy in homogeneoesly deformed Cu64.5Zr35.5 metallic glass

Using plastic deformation tests and high-energy X-ray scattering, we examined the anelastic strain and Structural anisotropy in a binary Cu64.5Zr35.5 metallic glass deformed homogeneously under uniaxial compression at 425 degrees C, which is approximately 60 degrees C below the glass transition temperature. For a sample quenched immediately after deformation, we find that the atomic Structure observed by X-ray scattering is anisotropic with the average bond length parallel to the loading axis being smaller than that of an undeformed, structurally relaxed reference sample, while the average bond length normal to the loading axis is dilated relative to the same reference sample. For it different sample annealed at 425 degrees C for 500 s immediately following deformation, the magnitude of the structural anisotropy decreases as anelastic strain is recovered. The relationship between the atomic-scale Structural rearrangements that occur during annealing and the macroscopic anelastic strain recovery is discussed. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved

Diffusion of Cu in AlCu alloys of different composition by quasielastic neutron scattering

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Structure of molten Al–Si alloys

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Structure of molten Al–Si alloys

International audienc

Diffusion of Cu in AlCu alloys of different composition by quasielastic neutron scattering

International audienc

Synthesis of Cu 47 Ti 34 Zr 11 Ni 8 Bulk Metallic Glass by Warm Extrusion of Gas Atomized Powders

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