Direct evidence for a functional role of HLA-DRB1 and -DRB3 gene products in the recognition of Dermatophagoides spp. (house dust mite) by helper T lymphocytes

The contribution of the HLA-DRB1, -B3, and -BS gene products in the recognition of Dermatophagoides spp. (house dust mite) by helper T cells Isolated from an atopic individual (HLA-DRw12, DR7; DRw52b) with perennial rhinitis was investigated. Using a panel of histocompatlble and histoincompatible accessory cells, the restriction specificity obtained for a long term T cell suggested that a component of the dust mite reactive repertoire recognized antigen in association with DRB3 gene products. Ollgonucleotide DNA typing of the presenting cell panel demonstrated a correlation between the DRw52b allele and T cell responsiveness. Murine fibroblasts expressing DRw52b, but not DRw52a or -c molecules, presented antigen to both the T cell line and cloned T cells (DE26) derived from the line, Indicating that the supertypic specificity DRw52b was able to restrict recognition of dust mite antigens. Additional T cell clones (DE9 and DE41) also isolated from the line were restricted by the products of the B1 gene locus (DRw12B1) as determined by murine fibroblasts transfected with the appropriate HLA-DR genes. Clone DE9 was degenerate in Its restriction specificity, also recognizing dust mite presented by accessory cells expressing the DR2 subtypes. Presentation by fibroblasts transfected with DRw12B1, DR2Dw2B5 genes and EBV-transformed B cell lines expressing DR2Dw21B1 and -B5 indicated that the functional site restricting recognition may be associated with residues 70 and 71 of the DR/3 chain helical wall of the antigen combining site. Furthermore, we have recently demonstrated that both T cell clones DE9 and DE26 induce allergen dependent IgE synthesis in vitro. Thus these results demonstrate directly that the DRB1, -B3, and -B5 gene products are functional in the restriction of T cell recognition of dust mite antigen

Etude de la régulation de la synthèse des isocatalases chez saccharomyces cerevisiae

Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe

Etude de la régulation de la synthèse des isocatalases chez saccharomyces cerevisiae

Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe

Anabolisme de la catalase chez la levure: Analyse d'un mutant de type constitutif

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Catalase anabolism in yeast: Loss of regulation by oxygen of catalase apoprotein synthesis after mutation

A mutant of Saccharomyces cerevisiae which displays catalase activity when grown under strictly anaerobic conditions has been selected on solid media. Although some preformed holoenzyme has accumulated in anaerobic cells, a sharp increase of activity is still measured during adaptation to oxygen in glucose-buffer; however, a striking difference with the wild-type strain is that in the mutant, catalase formation is observed in the presence of cycloheximide that totally inhibits cytoplasmic translation. It is concluded that kat 80 mutant has lost the regulatory control by oxygen of apocatalase synthesis; the latter precursor, characterized as apocatalase T, is thought to be activated in vivo, under aerobic conditions, by inclusion of prosthetic group. Regulation of enzyme synthesis by catabolite repression (glucose effect) persists, unmodified by reference to the wild-type parental strain. Mutation kat 80 specifically hits catalase anabolism, as no significant variations were observed for the edification of the respiratory system and (apo)cytochrome c peroxidase production. Genetic analysis shows that kat 80 phenotype, recessive in heterozygotes, results from a single nuclear mutation. © 1979 Springer-Verlag.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Global patterns and predictors of microplastic occurrence and abundance in lentic systems

The majority of microplastic research has focused on seawater, with fewer than 4% of microplasticsrelated studies occurring on freshwaters. The limited available information suggests that the abundance of microplastics in freshwaters is often as high or even higher than marine environments. However, comprehensive investigations on occurrence and fate of microplastics in freshwaters are scarce and highly fragmented, partly because detection and identification of microplastic particles is rather complex. In addition, up to now, harmonized and standardized protocols for the sampling and analysis of microplastics in freshwaters do not exist, and studies with different research aims and hypotheses often report unstandardized results, making comparison among studies difficult. In the present study, we performed the first global standardized sampling and analysis effort to investigate the occurrence and distribution of microplastics in surface water of lakes and reservoirs with different anthropogenic impacts. Participants aim to collect water samples of freshwater systems with different features (e.g., area, depth, thermal behavior, watershed), following a common protocol. This 18 establishes the collection of samples by horizontal trawling of a plankton net and, after treatment with hydrogen peroxide, the polymer identification through micro-Raman spectroscopy. This GLEON project will allow obtaining comparable data about microplastic contamination in different freshwater systems around the globe. With this global dataset, our goals are to determine whether a relationship exists between the abundance of microplastics and the waterbody/watershed attributes and understand which factors are likely to influence the occurrence of microplastics in surface water of lentic systems.info:eu-repo/semantics/publishedVersio

Global patterns and predictors of microplastic occurrence and abundance in lentic systems

The majority of microplastic research has focused on seawater, with fewer than 4% of microplasticsrelated studies occurring on freshwaters. The limited available information suggests that theabundance of microplastics in freshwaters is often as high or even higher than marine environments.However, comprehensive investigations on occurrence and fate of microplastics in freshwaters arescarce and highly fragmented, partly because detection and identification of microplastic particles israther complex. In addition, up to now, harmonized and standardized protocols for the sampling andanalysis of microplastics in freshwaters do not exist, and studies with different research aims andhypotheses often report unstandardized results, making comparison among studies difficult. In thepresent study, we performed the first global standardized sampling and analysis effort to investigatethe occurrence and distribution of microplastics in surface water of lakes and reservoirs with differentanthropogenic impacts. Participants aim to collect water samples of freshwater systems with differentfeatures (e.g., area, depth, thermal behavior, watershed), following a common protocol. This establishes the collection of samples by horizontal trawling of a plankton net and, after treatmentwith hydrogen peroxide, the polymer identification through micro-Raman spectroscopy. This GLEONproject will allow obtaining comparable data about microplastic contamination in different freshwatersystems around the globe. With this global dataset, our goals are to determine whether a relationshipexists between the abundance of microplastics and the waterbody/watershed attributes andunderstand which factors are likely to influence the occurrence of microplastics in surface water oflentic systems.Fil: Nava, Veronica. University Of Milano-Bicocca; ItaliaFil: Frezzotti, Maria Luce. University Of Milano-Bicocca; ItaliaFil: Aherne, Julian. Trent University; CanadáFil: Alfonso, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: AntãoGeraldes, Ana M.. Instituto Politécnico De Bragança; PortugalFil: Attermeyer, Katrin. No especifíca;Fil: Bah, Abdou R.. City University of New York; Estados UnidosFil: Bao, Roberto. Universidad da Coruña; EspañaFil: Bartrons, Mireia. Universidad Politécnica de Catalunya; EspañaFil: Berger, Stella A.. Leibniz Institute for Zoo and Wildlife Research; AlemaniaFil: Biernaczyk, Marcin. No especifíca;Fil: Breider, Florian. Institute Of Environmental Engineering; SueciaFil: Brookes, Justin. University of Adelaide; AustraliaFil: Cañedo Argüelles, Miguel. Universidad de Barcelona; EspañaFil: Canle, Moisés. Universidad da Coruña; EspañaFil: Capelli, Camilla. Institute Of Earth Sciences; SueciaFil: Cereijo, José. Universidad da Coruña; EspañaFil: Carballeira, Rafael. Universidad da Coruña; EspañaFil: Christensen, Søren T.. Cphbusiness Laboratory And Environment; DinamarcaFil: Christoffersen, Kirsten S.. Universidad de Copenhagen; DinamarcaFil: Clayer, Francois. Norwegian Institute For Water Research; NoruegaFil: De Eyto, Elvira. Marine Institute; MayotteFil: De Senerpont Domis, Lisette N.. Netherlands Institute of Ecology; Países BajosFil: Jordi Delgado, Martín. Universidad da Coruña; EspañaFil: Doubek, Jonathan P.. Lake Superior State University; Estados UnidosFil: Eaton, Ashley. University of Vermont; Estados UnidosFil: Erdogan, Seyda. Bozok University; TurquíaFil: Erina, Oxana. Lomonosov Moscow State University; RusiaFil: Ersoy, Zeynep. Universidade De Évora; PortugalFil: Feuchtmayr, Heidrun. No especifíca;Fil: Fugère, Vincent. Université du Québec a Montreal; CanadáFil: Galafassi, Silvia. Water Research Institute; ItaliaFil: Gonçalves, Vitor. Universidad de Porto. Facultad de Ciências. Centro de Investigação em Biodiversidade e Recursos Genéticos; PortugalFil: Grossart, Hans Peter. No especifíca;Fil: Hamilton, David. Griffith University; AustraliaFil: Hanson, Paul C.. University of Wisconsin; Estados UnidosFil: Harris, Ted. University of Kansas; Estados UnidosFil: Başaran Kankılıç, Gökben. Kırıkkale University; TurquíaFil: Kessler, Rebecca. University of Kansas; Estados UnidosFil: Jacquet, Stéphan. No especifíca;Fil: Kangur, Külli. Estonian University Of Life Sciences; EstoniaFil: Kiel, Christine. No especifíca;Fil: Knoll, Lesley. University of Minnesota; Estados UnidosFil: Kokorīte, Ilga. No especifíca;Fil: Lavoie, Isabelle. Institut National De La Recherche Scientifique; CanadáFil: Leiva Presa, Àngels. No especifíca;Fil: Lepori, Fabio. University Of Applied Sciences And Arts Of Southern Switzerland; SuizaFil: Lusher, Amy. Norwegian Institute For Water Research; NoruegaFil: Macintyre, Sally. University of California; Estados UnidosFil: Matias, Miguel. Universidade De Évora; PortugalFil: Matsuzaki, Shin Ichiro S.. National Institute For Enviromental Studies; JapónFil: Mccarthy, Valerie. Dundalk Institute Of Technology; IrlandaFil: Mcelarney, Yvonne. Agri - Food And Bioscience Institute; IrlandaFil: Mcnally, David. Winooski High School; Estados UnidosFil: Mekonen Belay, Berte. Netherlands Institute Of Ecology; Países BajosFil: Messyasz, Beata. Adam Mickiewicz University In Poznan; PoloniaFil: Mlambo, Musa. Rhodes University. Albany Museum.; SudáfricaFil: Nandini, Sarma. Universidad Nacional Autónoma de México; MéxicoFil: Nodine, Emily. Rollins College; Estados UnidosFil: Özen, Arda. Çankırı Karatekin University; TurquíaFil: Ozkundakci, Deniz. Waikato Regional Council Science; Nueva ZelandaFil: Vazquez Perez, Ricardo. Universidad da Coruña; EspañaFil: Pociecha, Agnieszka. Institute Of Nature Conservation; PoloniaFil: Raposeiro, Pedro. Universidad de Porto. Facultad de Ciências. Centro de Investigação em Biodiversidade e Recursos Genéticos; PortugalFil: Rõõm, Eva Ingrid. Estonian University Of Life Sciences; EstoniaFil: Salmaso, Nico. Research And Innovation Centre; ItaliaFil: Sarma, Singaraju S. S.. Facultad De Estudios Superiores Iztacala; MéxicoFil: Saulnier Talbot, Émilie. Laval University. Centre Hospitalier de L'universite Laval; CanadáFil: Scordo, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Sibomana, Claver. University Of Burundi; BurundiFil: Stepanowska, Katarzyna. West Pomeranian University Of Technology In Szczecin; PoloniaFil: Tavşanoğlu, Ü. Nihan. Çankırı Karatekin University; TurquíaFil: Tolotti, Monica. Research And Innovation Centre; ItaliaFil: Udoh, Abel. Gazi University; TurquíaFil: Urrutia Cordera, Pablo. Uppsala University; SueciaFil: Valois, Amanda. National Institute Of Water And Atmospheric Research; Nueva ZelandaFil: Vandergoes, Marcus. No especifíca;Fil: Verburg, Piet. National Institute Of Water And Atmospheric Research; Nueva ZelandaFil: Volta, Pietro. Water Research Institute - National Research Council; ItaliaFil: Wain, Danielle. No especifíca;Fil: Wesolek, Brian. Bay Mills Indian Community; Estados UnidosFil: Weyhenmeyer, Gesa. Uppsala University; SueciaFil: Wightman, Ryan. Agri - Food And Bioscience Institute; IrlandaFil: Wood, Susie. Cawthron Institute; Nueva ZelandaFil: Wu, Naicheng. Ningbo University; ChinaFil: Zawiska, Izabela. Polish Academy of Sciences; ArgentinaFil: Zawisza, Edyta. Polish Academy of Sciences; ArgentinaFil: Zink, Lauren. University Of Lethbridge; CanadáFil: Leoni, Barbara. University Of Milano Bicocca; ItaliaThe Global Lake Ecological Observatory Network All Hands' MeetingEstados UnidosGlobal Lake Ecological Observatory Networ

Women and health professionals’ perspectives on a conditional cash transfer programme to improve pregnancy follow-up: a qualitative analysis of the NAITRE randomised controlled study

Objectives Women of low socioeconomic status have been described as having suboptimal prenatal care, which in turn has been associated with poor pregnancy outcomes. Many types of conditional cash transfer (CCT) programmes have been developed, including programmes to improve prenatal care or smoking cessation during pregnancy, and their effects demonstrated. However, ethical critiques have included paternalism and lack of informed choice. Our objective was to determine if women and healthcare professionals (HPs) shared these concerns.Design Prospective qualitative research.Setting We included economically disadvantaged women, as defined by health insurance data, who participated in the French NAITRE randomised trial assessing a CCT programme during prenatal follow-up to improve pregnancy outcomes. The HP worked in some maternities participating in this trial.Participants 26 women, 14 who received CCT and 12 who did not, mostly unemployed (20/26), and - 7 HPs.Interventions We conducted a multicentre cross-sectional qualitative study among women and HPs who participated in the NAITRE Study to assess their views on CCT. The women were interviewed after childbirth.Results Women did not perceive CCT negatively. They did not mention feeling stigmatised. They described CCT as a significant source of aid for women with limited financial resources. HP described the CCT in less positive terms, for example, expressing concern about discussing cash transfer at their first medical consultation with women. Though they emphasised ethical concerns about the basis of the trial, they recognised the importance of evaluating CCT.Conclusions In France, a high-income country where prenatal follow-up is free, HPs were concerned that the CCT programme would change their relationship with patients and wondered if it was the best use of funding. However, women who received a cash incentive said they did not feel stigmatised and indicated that these payments helped them prepare for their baby’s birth.Trial registration number NCT0240285