Fission yeast obeys a linear size law under nutrient titration

Steady-state cell size and geometry depend on growth conditions. Here, we use an experimental setup based on continuous culture and single-cell imaging to study how cell volume, length, width and surface-to-volume ratio vary across a range of growth conditions including nitrogen and carbon titration, the choice of nitrogen source, and translation inhibition. Overall, we find cell geometry is not fully determined by growth rate and depends on the specific mode of growth rate modulation. However, under nitrogen and carbon titrations, we observe that the cell volume and the growth rate follow the same linear scaling

STL-based Analysis of TRAIL-induced Apoptosis Challenges the Notion of Type I/Type II Cell Line Classification

Extrinsic apoptosis is a programmed cell death triggered by external ligands, such as the TNF-related apoptosis inducing ligand (TRAIL). Depending on the cell line, the specific molecular mechanisms leading to cell death may significantly differ. Precise characterization of these differences is crucial for understanding and exploiting extrinsic apoptosis. Cells show distinct behaviors on several aspects of apoptosis, including (i) the relative order of caspases activation, (ii) the necessity of mitochondria outer membrane permeabilization (MOMP) for effector caspase activation, and (iii) the survival of cell lines overexpressing Bcl2. These differences are attributed to the activation of one of two pathways, leading to classification of cell lines into two groups: type I and type II. In this work we challenge this type I/type II cell line classification. We encode the three aforementioned distinguishing behaviors in a formal language, called signal temporal logic (STL), and use it to extensively test the validity of a previously-proposed model of TRAIL-induced apoptosis with respect to experimental observations made on different cell lines. After having solved a few inconsistencies using STL-guided parameter search, we show that these three criteria do not define consistent cell line classifications in type I or type II, and suggest mutants that are predicted to exhibit ambivalent behaviors. In particular, this finding sheds light on the role of a feedback loop between caspases, and reconciliates two apparently-conflicting views regarding the importance of either upstream or downstream processes for cell-type determination. More generally, our work suggests that these three distinguishing behaviors should be merely considered as type I/II features rather than cell-type defining criteria. On the methodological side, this work illustrates the biological relevance of STL-diagrams, STL population data, and STL-guided parameter search implemented in the tool Breach. Such tools are well-adapted to the ever-increasing availability of heterogeneous knowledge on complex signal transduction pathways

The HARPS search for southern extra-solar planets. XXIV. Companions to HD 85390, HD 90156 and HD 103197: A Neptune analogue and two intermediate mass planets

We report the detection of three new extrasolar planets orbiting the solar type stars HD 85390, HD 90156 and HD 103197 with the HARPS spectrograph mounted on the ESO 3.6-m telescope at La Silla observatory. HD 85390 has a planetary companion with a projected intermediate mass (42.0 Earth masses) on a 788-day orbit (a=1.52 AU) with an eccentricity of 0.41, for which there is no analogue in the solar system. A drift in the data indicates the presence of another companion on a long period orbit, which is however not covered by our measurements. HD 90156 is orbited by a warm Neptune analogue with a minimum mass of 17.98 Earth masses (1.05 Neptune masses), a period of 49.8 days (a=0.25 AU) and an eccentricity of 0.31. HD 103197 has an intermediate mass planet on a circular orbit (P=47.8 d, Msini=31.2 Earth masses). We discuss the formation of planets of intermediate mass (about 30-100 Earth masses) which should be rare inside a few AU according to core accretion formation models.Comment: 9 pages, 5 figures. Accepted to A&

Io: IUE observations of its atmosphere and the plasma torus

Two of the main components of the atmosphere of Io, neutral oxygen and sulfur, were detected with the IUE. Four observations yield brightnesses that are similar, regardless of whether the upstream or the downstream sides of the torus plasma flow around Io is observed. A simple model requires the emissions to be produced by the interaction of O and S columns in the exospheric range with 2 eV electrons. Cooling of the 5 eV torus electrons is required prior to their interaction with the atmosphere of Io. Inconsistencies in the characteristics of the spectra that cannot be accounted for in this model require further analysis with improved atomic data. The Io plasma torus was monitored with the IUE. The long-term stability of the warm torus is established. The observed brightnesses were analyzed using a model of the torus, and variations of less than 30 percent in the composition are observed, the quantitative results being model dependent

The HARPS search for southern extra-solar planets. XXVII. Up to seven planets orbiting HD 10180: probing the architecture of low-mass planetary systems

Context. Low-mass extrasolar planets are presently being discovered at an increased pace by radial velocity and transit surveys, opening a new window on planetary systems. Aims. We are conducting a high-precision radial velocity survey with the HARPS spectrograph which aims at characterizing the population of ice giants and super-Earths around nearby solar-type stars. This will lead to a better understanding of their formation and evolution, and yield a global picture of planetary systems from gas giants down to telluric planets. Methods. Progress has been possible in this field thanks in particular to the sub-m/s radial velocity precision achieved by HARPS. We present here new high-quality measurements from this instrument. Results. We report the discovery of a planetary system comprising at least five Neptune-like planets with minimum masses ranging from 12 to 25 M_Earth, orbiting the solar-type star HD 10180 at separations between 0.06 and 1.4 AU. A sixth radial velocity signal is present at a longer period, probably due to a 65-M_Earth object. Moreover, another body with a minimum mass as low as 1.4 M_Earth may be present at 0.02 AU from the star. This is the most populated exoplanetary system known to date. The planets are in a dense but still well-separated configuration, with significant secular interactions. Some of the orbital period ratios are fairly close to integer or half-integer values, but the system does not exhibit any mean-motion resonances. General relativity effects and tidal dissipation play an important role to stabilize the innermost planet and the system as a whole. Numerical integrations show long-term dynamical stability provided true masses are within a factor ~3 from minimum masses. We further note that several low-mass planetary systems exhibit a rather "packed" orbital architecture with little or no space left for additional planets. (Abridged)Comment: 20 pages, 15 figures, accepted for publication in A&

First climatology of polar mesospheric clouds from GOMOS/ENVISAT stellar occultation instrument

GOMOS (Global Ozone Monitoring by Occultation of Stars), on board the European platform ENVISAT launched in 2002, is a stellar occultation instrument combining four spectrometers and two fast photometers which measure light at 1 kHz sampling rate in the two visible channels 470–520 nm and 650–700 nm. On the day side, GOMOS does not measure only the light from the star, but also the solar light scattered by the atmospheric molecules. In the summer polar days, Polar Mesospheric Clouds (PMC) are clearly detected using the photometers signals, as the solar light scattered by the cloud particles in the instrument field of view. The sun-synchronous orbit of ENVISAT allows observing PMC in both hemispheres and the stellar occultation technique ensures a very good geometrical registration. Four years of data, from 2002 to 2006, are analyzed up to now. GOMOS data set consists of approximately 10 000 cloud observations all over the eight PMC seasons studied. The first climatology obtained by the analysis of this data set is presented, focusing on the seasonal and latitudinal coverage, represented by global maps. GOMOS photometers allow a very sensitive PMC detection, showing a frequency of occurrence of 100% in polar regions during the middle of the PMC season. According to this work mesospheric clouds seem to be more frequent in the Northern Hemisphere than in the Southern Hemisphere. The PMC altitude distribution was also calculated. The obtained median values are 82.7 km in the North and 83.2 km in the South

The Nature and Frequency of the Gas Outbursts in Comet 67P/Churyumov-Gerasimenko observed by the Alice Far-ultraviolet Spectrograph on Rosetta

Alice is a far-ultraviolet imaging spectrograph onboard Rosetta that, amongst multiple objectives, is designed to observe emissions from various atomic and molecular species from within the coma of comet 67P/Churyumov-Gerasimenko. The initial observations, made following orbit insertion in August 2014, showed emissions of atomic hydrogen and oxygen spatially localized close to the nucleus and attributed to photoelectron impact dissociation of H2O vapor. Weaker emissions from atomic carbon were subsequently detected and also attributed to electron impact dissociation, of CO2, the relative H I and C I line intensities reflecting the variation of CO2 to H2O column abundance along the line-of-sight through the coma. Beginning in mid-April 2015, Alice sporadically observed a number of outbursts above the sunward limb characterized by sudden increases in the atomic emissions, particularly the semi-forbidden O I 1356 multiplet, over a period of 10-30 minutes, without a corresponding enhancement in long wavelength solar reflected light characteristic of dust production. A large increase in the brightness ratio O I 1356/O I 1304 suggests O2 as the principal source of the additional gas. These outbursts do not correlate with any of the visible images of outbursts taken with either OSIRIS or the navigation camera. Beginning in June 2015 the nature of the Alice spectrum changed considerably with CO Fourth Positive band emission observed continuously, varying with pointing but otherwise fairly constant in time. However, CO does not appear to be a major driver of any of the observed outbursts.Comment: 6 pages, 4 figures, accepted for publication in the Astrophysical Journal Letter

Alice: The Rosetta Ultraviolet Imaging Spectrograph

We describe the design, performance and scientific objectives of the NASA-funded ALICE instrument aboard the ESA Rosetta asteroid flyby/comet rendezvous mission. ALICE is a lightweight, low-power, and low-cost imaging spectrograph optimized for cometary far-ultraviolet (FUV) spectroscopy. It will be the first UV spectrograph to study a comet at close range. It is designed to obtain spatially-resolved spectra of Rosetta mission targets in the 700-2050 A spectral band with a spectral resolution between 8 A and 12 A for extended sources that fill its ~0.05 deg x 6.0 deg field-of-view. ALICE employs an off-axis telescope feeding a 0.15-m normal incidence Rowland circle spectrograph with a concave holographic reflection grating. The imaging microchannel plate detector utilizes dual solar-blind opaque photocathodes (KBr and CsI) and employs a 2 D delay-line readout array. The instrument is controlled by an internal microprocessor. During the prime Rosetta mission, ALICE will characterize comet 67P/Churyumov-Gerasimenko's coma, its nucleus, and the nucleus/coma coupling; during cruise to the comet, ALICE will make observations of the mission's two asteroid flyby targets and of Mars, its moons, and of Earth's moon. ALICE has already successfully completed the in-flight commissioning phase and is operating normally in flight. It has been characterized in flight with stellar flux calibrations, observations of the Moon during the first Earth fly-by, and observations of comet Linear T7 in 2004 and comet 9P/Tempel 1 during the 2005 Deep Impact comet-collision observing campaignComment: 11 pages, 7 figure

Growth-rate dependent and nutrient-specific gene expression resource allocation in fission yeast

Cellular resources are limited and their relative allocation to gene expression programmes determines physiological states and global properties such as the growth rate. Here, we determinedtheimportanceof the growth rate in explaining relative changes in protein and mRNA levels in the simple eukaryote Schizosaccharomyces pombegrownon non-limiting nitrogen sources. Although expression of half of fission yeast genes was significantly correlated with the growth rate, this came alongside wide-spread nutrient-specific regulation. Proteome and transcriptome often showed coordinated regulation but with notable exceptions, such as metabolic enzymes. Genes positively correlated with growth rate participated in every level of protein production apart fromRNA polymerase II-dependent transcription.Negatively correlated genes belonged mainly to the environmental stress response programme. Critically, metabolic enzymes, which represent ~55-70% of the proteome by mass,showedmostly condition-specificregulation.In summary, we provide a rich account of resource allocation to gene expression in a simple eukaryote, advancing our 19basic understanding of the interplay between growth-rate dependent and nutrient-specific gene expression

The HARPS search for southern extra-solar planets. VI. A Neptune-mass planet around the nearby M dwarf Gl 581

We report the discovery of a Neptune-mass planet around Gl 581 (M3V, M = 0.31 Msol), based on precise Doppler measurements with the HARPS spectrograph at La Silla Observatory. The radial velocities reveal a circular orbit of period P = 5.366 days and semi-amplitude K1 = 13.2 m/s. The resulting minimum mass of the planet (m2 sin i) is only 0.052 Mjup = 0.97 Mnep = 16.6 Mearth making Gl 581b one of the lightest extra-solar planet known to date. The Gl 581 planetary system is only the third centered on an M dwarf, joining the Gl 876 three-planet system and the lone planet around Gl 436. Its discovery reinforces the emerging tendency of such planets to be of low mass, and found at short orbital periods. The statistical properties of the planets orbiting M dwarfs do not seem to match a simple mass scaling of their counterparts around solar-type stars.Comment: letter submitted to A&