Diversite Phlebotomienne Dans Trois Villages De La Commune De Bouake (Côte D’ivoire) Durant La Periode De Mars A Juin 2019

La leishmaniose cutanée est une parasitose due à des protozoaires du genre Leishmania et répandue dans le monde. Une enquête médicale réalisée dans trois villages de la ville de Bouaké, autour de cas suspects de leishmaniose cutanée, a permis de confirmer la présence de cette pathologie dans ces villages. Dans le cadre de l’identification des acteurs du cycle épidémiologique de la leishmaniose cutanée, une étude entomologique a été conduite dans ces villages afin d’identifier les phlébotomes potentiels vecteurs de leishmanies. Les pièges huileux et les pièges lumineux CDC, ont été posés entre mars et juin 2019, suivant une méthode de rotation entre les sites de piégeage. Ils ont été posés entre 17h et 18h et relevés le lendemain matin entre 7h et 8h. La diversité spécifique des espèces identifiées a été déterminée à partir des indices écologiques d’équitabilité et de Hill. Ces pièges ont permis la capture de 135 phlébotomes, dont 78 ont été morphologiquement identifiés. Le genre Sergentomyia constituait 91% de nos récoltes contre 9% pour le genre Phlebotomus. Ph. bergeroti, Ph. rodhaini et Ph. sergenti étaient les espèces du genre Phlebotomus, capturées dans ces sites. Des travaux approfondis portant sur l’identification du parasite à la fois chez les phlébotomes et chez l’homme doivent être effectués, afin d’identifier les phlébotomes incriminés dans la transmission des leishmanies à Bouaké. Cutaneous leishmaniasis is a parasitosis caused by protozoa of the genus Leishmania and is widespread worldwide. A medical survey carried out in three villages of the city of Bouaké, around suspected cases of cutaneous leishmaniasis, confirmed the presence of this pathology in these villages. As part of the identification of the actors of the epidemiological cycle of cutaneous leishmaniasis, an entomological study was conducted in these villages in order to identify potential phlebotomus vectors of leishmaniasis. Oil traps and CDC light traps were set between March and June 2019, following a rotation method between trapping sites. They were set between 5:00 pm and 6:00 pm and were collected the next morning between 7:00 am and 8:00 am. The specific diversity of the identified species was determined from the ecological indices of equitability and Hill's ecological indices. These traps allowed the capture of 135 sandfish, 78 of which were morphologically identified. The genus Sergentomyia constituted 91% of our harvests against 9% for the genus Phlebotomus. Ph. bergeroti, Ph. rodhaini and Ph. sergenti were the species of the genus Phlebotomus caught at these sites. Further work on the identification of the parasite in both sandflies and humans should be carried out in order to identify the sandflies incriminated in the transmission of leishmania in Bouaké

Use of vector control to protect people from sleeping sickness in the focus of Bonon (Côte d’Ivoire)

Background Gambian human African trypanosomiasis (gHAT) is a neglected tropical disease caused by Trypanosoma brucei gambiense transmitted by tsetse flies (Glossina). In Côte d’Ivoire, Bonon is the most important focus of gHAT, with 325 cases diagnosed from 2000 to 2015 and efforts against gHAT have relied largely on mass screening and treatment of human cases. We assessed whether the addition of tsetse control by deploying Tiny Targets offers benefit to sole reliance on the screen-and-treat strategy. Methodology and principal findings In 2015, we performed a census of the human population of the Bonon focus, followed by an exhaustive entomological survey at 278 sites. After a public sensitization campaign, ~2000 Tiny Targets were deployed across an area of 130 km2 in February of 2016, deployment was repeated annually in the same month of 2017 and 2018. The intervention’s impact on tsetse was evaluated using a network of 30 traps which were operated for 48 hours at three-month intervals from March 2016 to December 2018. A second comprehensive entomological survey was performed in December 2018 with traps deployed at 274 of the sites used in 2015. Sub-samples of tsetse were dissected and examined microscopically for presence of trypanosomes. The census recorded 26,697 inhabitants residing in 331 settlements. Prior to the deployment of targets, the mean catch of tsetse from the 30 monitoring traps was 12.75 tsetse/trap (5.047–32.203, 95%CI), i.e. 6.4 tsetse/trap/day. Following the deployment of Tiny Targets, mean catches ranged between 0.06 (0.016–0.260, 95%CI) and 0.55 (0.166–1.794, 95%CI) tsetse/trap, i.e. 0.03–0.28 tsetse/trap/day. During the final extensive survey performed in December 2018, 52 tsetse were caught compared to 1,909 in 2015, with 11.6% (5/43) and 23.1% (101/437) infected with Trypanosoma respectively. Conclusions The annual deployment of Tiny Targets in the gHAT focus of Bonon reduced the density of Glossina palpalis palpalis by >95%. Tiny Targets offer a powerful addition to current strategies towards eliminating gHAT from Côte d’Ivoire

Morphological characterization of ticks of the genus <em>Rhipicephalus (Boophilus)</em> based on geometric morphometry: the case of the Savannah District in Côte d’Ivoire

The introduction of the invasive tick Rhipicephalus (Boophilus) microplus in Côte d'Ivoire has led to a change in the population of ticks of the genus Rhipicephalus (Boophilus). Thus, this study aims to characterize morphologically the tick species of the genus Rhipicephalus (B.) by geometric morphometry. It consisted of collecting all ticks from cattle in 74 farms in the Savanes District. The collected ticks were identified in the laboratory and, after identification, only non-gorged adult ticks of the genus Rhipicephalus (B.) were retained for morphometric characterisation. A total of 394 ticks were examined under a digital microscope, images were digitised using the online program XYOM and the data were analysed. This study revealed that males of each species have a smaller average size than females. It also showed a variation in mean size in all species except for the females of Rhipicephalus (B.) decoloratus and Rhipicephalus (B.) geigyi whose mean sizes did not differ. Geometric morphometry allowed the separation of Rhipicephalus (B.) annulatus males from other males. In females, Rhipicephalus (B.) geigyi and Rhipicephalus (B.) microplus were distinguished from each other and from other females