Feeding behavior and activity of Phlebotomus pedifer and potential reservoir hosts of Leishmania aethiopica in southwestern Ethiopia

Background Cutaneous leishmaniasis (CL) is a major public health concern in Ethiopia. However, knowledge about the complex zoonotic transmission cycle is limited, hampering implementation of control strategies. We explored the feeding behavior and activity of the vector (Phlebotomus pedifer) and studied the role of livestock in CL transmission in southwestern Ethiopia. Methods Blood meal origins of engorged sand flies were determined by sequencing host DNA. A host choice experiment was performed to assess the feeding preference of P. pedifer when humans and hyraxes are equally accessible. Ear and nose biopsies from livestock were screened for the presence of Leishmania parasites. Sand flies were captured indoor and outdoor with human landing catches and CDC light traps to determine at which time and where P. pedifer is mostly active. Principal findings A total of 180 P. pedifer sand flies were found to bite hosts of 12 genera. Humans were the predominant blood meal source indoors (65.9%, p < 0.001), while no significant differences were determined outdoors and in caves. In caves, hyraxes were represented in blood meals equally as humans (45.5% and 42.4%, respectively), but the host choice experiment revealed that sand flies have a significant preference for feeding on hyraxes (p = 0.009). Only a single goat nose biopsy from 412 animal samples was found with Leishmania RNA. We found that P. pedifer is predominantly endophagic (p = 0.003), but occurs both indoors and outdoors. A substantial number of sand flies was active in the early evening, which increased over time reaching its maximum around midnight. Conclusion In contrast to earlier suggestions of exclusive zoonotic Leishmania transmission, we propose that there is also human-to-human transmission of CL in southwestern Ethiopia. Livestock does not play a role in CL transmission and combined indoor and outdoor vector control measures at night are required for efficient vector control.publishedVersio

Mouse Aortic Ring Assay: A New Approach of the Molecular Genetics of Angiogenesis

Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloproteases in angiogenesis, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report here that this model is attractive to elucidate the cellular and molecular mechanisms of angiogenesis, to identify, characterise or screen "pro- or anti-angiogenic agents that could be used for the treatment of angiogenesis-dependent diseases. Approaches include using recombinant proteins, synthetic molecules and adenovirus-mediated gene transfer

Schwanke Engines E-85 Sealed Engine Project

The objective of our project is to test E-85 and observe its potential for racing use in a 5.3-liter spec, engine. We will be testing and researching E-85 using the Minnesota State University Mankato facilities. Our group will be collaborating with local race engine builder Tim Schwanke from Springfield, Minnesota. Mr. Schwanke is on the verge of starting a sealed engine race class at the local dirt tracks. He is trying to make an affordable race class with the use of a sealed, low cost, highly reliable engine. In , addition to saving substantial amounts of money by having -, a sealed engine, Tim is also looking at lower cost fuel , alternatives. One of the fuels that make financial sense is ethanol. Die price of ethanol is usually comparable to normal pump gas, but has the higher octane benefits of \u27 racing fuel. We will be researching and testing E-85 by comparison with race gasoline and methanol to discover if ethanol will be a feasible alternative to using normal race fuels

Design considerations for interactive office lighting interface characteristics, shared and hybrid control

The inclusion of IoT in office lighting allows people to have personal lighting control at their workplace. To design lighting control interfaces that fit people’s everyday living, we need a better understanding of how people experience lighting interaction in the real world. Still, lighting control is often explored in controlled settings. This work presents a qualitative field study concerning the user experience of two control interfaces for a state-of-the-art lighting system of 400+ luminaires in a real-life office. In ten weeks, 43 people interacted 3937 times. The findings illustrate the effects of using a smartphone for lighting control, how people experience lighting control in shared situations, and issues with automatic system behavior. We define design considerations for interface characteristics, shared control, and hybrid control. The work contributes to making the potential benefits of interactive office lighting a reality

Do unions always decrease wage dispersion? The case of Brazilian manufacturing

The literature on the impact of unions on wages has established that unionized workers earn a wage premium when compared to their nonunion counterparts and that the dispersion of wages within the union sector is lower than in the nonunion sector. I examine the validity of these findings in the context of a developing country labor market and show that unionism does create a positive wage differential but that wage dispersion is greater in the union sector These findings are explained by the greater variance in the characteristics of unionized workers, the vulnerability of nonunion workers to market conditions, and the structure of wage bargaining