Comparação clínica e tomográfica de implantes dentários instalados de forma convencional e virtualmente guiados

TCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências da Saúde. Odontologia.Este trabalho tem por objetivo avaliar a diferença angular do posicionamento dos implantes dentários entre a cirurgia virtual guiada e a convencional a campo aberto, visando à constatação da técnica cirúrgica mais fácil, de maior precisão e com menor morbidade para o paciente. Foram selecionados 04 pacientes do sexo feminino, leucodermas, entre 45 e 55 anos, com ausência contralateral de dentes, onde cada lado foi operado por meio de uma técnica. Posteriormente, foi feita a comparação das imagens tomográficas do planejamento e da posição real dos implantes, por meio do software Dental Slice. De acordo com o teste estatístico, foi possível verificar que o valor de desvio angular é estatisticamente menor (p<0,05) na cirurgia virtual guiada. Os resultados obtidos demonstraram maior precisão e previsibilidade na técnica virtual guiada, quando comparada a técnica convencional

Does the number of implants have any relation with peri-implant disease?

Objective: The aim of this study was to evaluate the relationship between the number of pillar implants of implant-supported fixed prostheses and the prevalence of periimplant disease. Material and Methods: Clinical and radiographic data were obtained for the evaluation. The sample consisted of 32 patients with implant-supported fixed prostheses in function for at least one year. A total of 161 implants were evaluated. Two groups were formed according to the number of implants: G1) ≤5 implants and G2) >;5 implants. Data collection included modified plaque index (MPi), bleeding on probing (BOP), probing depth (PD), width of keratinized mucosa (KM) and radiographic bone loss (BL). Clinical and radiographic data were grouped for each implant in order to conduct the diagnosis of mucositis or peri-implantitis. Results: Clinical parameters were compared between groups using Student’s t test for numeric variables (KM, PD and BL) and Mann-Whitney test for categorical variables (MPi and BOP). KM and BL showed statistically significant differences between both groups (

Does the number of implants have any relation with peri-implant disease?

Objective: The aim of this study was to evaluate the relationship between the number of pillar implants of implant-supported fixed prostheses and the prevalence of periimplant disease. Material and Methods: Clinical and radiographic data were obtained for the evaluation. The sample consisted of 32 patients with implant-supported fixed prostheses in function for at least one year. A total of 161 implants were evaluated. Two groups were formed according to the number of implants: G1) ≤5 implants and G2) >5 implants. Data collection included modified plaque index (MPi), bleeding on probing (BOP), probing depth (PD), width of keratinized mucosa (KM) and radiographic bone loss (BL). Clinical and radiographic data were grouped for each implant in order to conduct the diagnosis of mucositis or peri-implantitis. Results: Clinical parameters were compared between groups using Student’s t test for numeric variables (KM, PD and BL) and Mann-Whitney test for categorical variables (MPi and BOP). KM and BL showed statistically significant differences between both groups (p<0.001). Implants from G1 – 19 (20.43%) – compared with G2 – 26 (38.24%) – showed statistically significant differences regarding the prevalence of peri-implantitis (p=0.0210). Conclusion: It seems that more than 5 implants in total fixed rehabilitations increase bone loss and consequently the prevalence of implants with periimplantitis. Notwithstanding, the number of implants does not have any influence on the prevalence of mucositis

Influência do momento e posicionamento de instalação do implante na formação óssea peri-implantar em diferentes tratamentos de superfície

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Odontologia, Florianópolis, 2014.Objetivos: verificar a influência do momento de instalação e do posicionamento do implante, bem como a presença ou não do GAP vestibular, associado ao seu tratamento de superfície, na formação óssea ao redor dos implantes. Materiais e métodos: Em um primeiro momento 3 pré-molares de um lado da arcada de 07 cães mongrel foram extraídos. Após 120 dias de cicatrização alveolar, em um segundo ato cirúrgico neste sextante, foi elevado um retalho de espessura total e foram instalados 3 implantes. Na mesma cirurgia os pré-molares contralaterais foram extraídos e feita a instalação imediata de 3 implantes, por meio da técnica do approach palatino, sem o rebatimento de retalho, totalizando 6 implantes por animal. Os 42 implantes instalados formaram grupos de acordo com o momento (imediato ou tardio) da instalação do implante e do tratamento de superfície. Após 4 meses do segundo ato cirúrgico realizou-se a coleta das amostras e a análise histomorfométrica, afim de se observar: BIC vestibular, BIC lingual, BIC total, área vestibular e área lingual de todos os implantes. Para análise estatística utilizou-se os testes Kruskal-wallis e Wilcoxon pareado (p Abstract : Objective: To investigate the influence of time of placement and positioning of the implant, the presence or absence of the buccal GAP, associated with its surface treatment on bone formation around the implants. Materials and methods: At first 3 premolars from one side of the arch of 07 mongrel dogs were extracted. After 120 days of alveolar healing, in a second surgery on this side, a full flap was elevated and 3 implants were placed. At the same surgery, the contralateral premolars were extracted and a immediate placement of 3 implants were done using the flapless technique of palatal approach. Resulting in a total of 6 implants per animal. The groups were formed according to the time of implant placement and surface treatment. 4 months after the second surgery was performed the sample collection and histomorphometric analysis to investigate: buccal BIC, lingual BIC, full BIC, buccal area and lingual area of all implants. Results: After 4 months of healing, it was observed that groups related to immediate implants showed better rates of BIC, especially on the buccal bone. The data also suggest an advantage in the bone area around the implants in these same groups. About surface treatment, the best results were found for sand-blasting + acid attack. Conclusion: It is concluded that performing the flapless surgical technique with palatal approach, the surface treatment and the time of implant placement have favorable effect on bone formation around them

Compostos anti-biofilme: estudo in vitro em biofilmes mono e multi-espécies

Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Odontologia, Florianópolis, 2017.Objetivos: O presente estudo tem como objetivo avaliar a citotoxicidade das lactamas em queratinócitos orais humanos (HOK-18A) e sua atividade anti-biofilme contra biofilmes mono e multi-espécie, constituídos por 14 cepas bactérias da microbiota oral. Materiais e métodos: O efeito citotóxico das lactamas foi avaliado através do teste XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] em queratinócitos orais humanos (HOK-18A). Para os testes de atividade anti-biofilme, as lactamas foram testadas contra 5 espécies colonizadoras iniciais, 6 patógenos orais e 3 cepas comensais. Os testes em biofilme mono-espécie foram realizados através do protocolo fixação com etanol e coloração com cristal violeta. Os testes multi-espécie foram realizados em um biofilme complexo multi-espécies derivado de um biorreator. Tanto o crescimento planquitônico quanto a formação de biofilme foram mensurados através de q-pcr. Adicionalmente, o efeito das lactamas em biofilmes multi-espécie formado sobre duas superfícies diferentes também foi avaliado através de q-pcr. Resultados: De 26 lactamas testadas, 24 mantiveram mais de 50% de viabilidade celular após 24h de exposição e foram selecionadas para os testes seguintes. Em relação aos testes anti-biofilme mono-espécie, a maior parte das lactamas apresentou atividade de inibição do biofilme em S. oralis, S. sanguinis, A naeslundii, P. intermedia, P. gingivalis e F. nucleatum (pAbstract : Objectives: The present study aims to evaluate the cytotoxicity of human oral keratinocytes (HOK-18A) and its anti-biofilm activity against mono and multi-species biofilms of 14 bacterial strains of the oral microbiota. Materials and methods: The cytotoxic effect of lactams was assessed by XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] test in human oral keratinocytes (HOK- 18A). For the anti-biofilm activity tests, the lactams were tested against 5 initial colonizers species, 6 oral pathogens and 3 commensals strains. The tests on mono-species biofilm were performed through ethanol fixation protocol and crystal violet staining. The multi-species tests were performed on a derived-bioreactor multi-species complex biofilm. Both, planktonic growth and biofilm formation were quantified by q-pcr. Additionally, the effect of lactams on multi-species biofilms formed on two different surfaces was also evaluated by q-pcr. Results: 24 out of 26 lactams maintained more than 50% of viable cells after 24 hours of exposure and were selected for the following tests (p <0.05). Regarding mono-species anti-biofilm experiments, most of the lactams presented biofilm inhibition activity in S. oralis, S. sanguinis, A naeslundii, P. intermedia, P. gingivalis and F. nucleatum (p<0,05). Furthermore, 9, 3 and 1 lactams were able to decrease biofilm formation in A.a., S. mutans and S. sobrinus respectively (p<0.05). Although in multi-species biofilm, a slight decrease in the biofilm formation can be observed in S. sanguinis, A. naeslundii, S. mitis and S. gordonii, only in P. gingivalis and P. intermedia, the inhibition was statistically significant. However, when tested on glass disks, 7 species were inhibited while only 2 on a polystyrene surface. Conclusion: Lactams proved to be successful by the results obtained both in the cytotoxic and mono-especies biofilm inhibition assays. However the anti-biofilm effect in multi-species biofilm was decreased by the complexity of bacterial interactions. Although most of lactams did not have anti-biofilm activity against multi-species biofilms on both surfaces. Due to the higher effectiveness of the lactam U12 was only on glass discs biofilms, it can be suggested that surface have an influence on its potential

Antimicrobial effects of commensal oral species are regulated by environmental factors

The objectives of this study are to identify oral commensal species which can inhibit the growth of the main periodontopathogens, to determine the antimicrobial substances involved in these inhibitory activities and to evaluate the influence of environmental factors on the magnitude of these inhibitions.publisher: Elsevier articletitle: Antimicrobial effects of commensal oral species are regulated by environmental factors journaltitle: Journal of Dentistry articlelink: http://dx.doi.org/10.1016/j.jdent.2016.02.007 content_type: article copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.status: publishe