Autumn MIST 2017

Jasmine Sandhu, Georgina Graham, Sarah Bentley and John Coxon report on the annual Magnetosphere, Ionosphere, and Solar–Terrestrial (MIST) meeting, highlighting science results from the Cassini and Juno missions

Report of Virtual Conference. BASICS Phase I - Achievements and Learnings Meeting. May 18-19, 2020

The Building an Economically Sustainable Integrated Cassava Seed System in Nigeria (BASICS) project began in 2016 and formally ends on 30 June 2020. The project has made progress in demonstrating that commercially viable production and sale of breeder, foundation and certified seed is possible. Furthermore, the project has established a strong basis for building a sustainable seed system by developing building blocks across the seed value chain. This meeting had the following objectives: 1. To identify the achievements and lessons learned in each of the project components; 2. To identify the shortcomings in each component (what would I do differently, knowing what I know now?), remaining challenges and ideas to overcome them; 3. To assess and discuss the challenges and progress made in integrating the components into an integrated seed system and identify ways integration can be improved; 4. To assess and discuss the commercial sustainability of the seed system and identify options to promote its sustainability and further scaling; and 5. To make plans for the publication of the findings and lessons learned during BASICS-

Fluid Intake and Hydration Status of Forest Workers -- A Preliminary Investigation

Dehydration and its milder form hypohydration have both short term and long term health effects. In the short term poor, body hydration impairs cognitive performance, physical strength and aerobic power, rendering the worker prone to injury and heat illness. In the long term the potential consequences of hypohydration are kidney stones and bladder cancer. The aim of this study was to evaluate hydration status of forest workers in New Zealand and their preferred fluid replacement. The specific gravity (sg) of urine was used as an indicator of body fluid status. In addition daily fluid loss was compared with a tested algorithm of sweat rate to better understand if workers are hydrating at the desired rate. The results of this preliminary study clearly demonstrate that loggers are working at sub-optimal hydration levels and are consuming inappropriate fluids to replace sweat losses. The hypohydrated state of these workers may pose both an immediate and long term health and safety risk

Patterns of Ministry of clergy married to clergy in the Church of England

This is the author's manuscript of an article published in Journal of Anglican Studies.This article argues that for good practice, wellbeing and fruitful ministry, decisions by and about clergy married to clergy (CMC) in the Church of England require a clear quantitative picture of their ministry, and offers such a picture in early 2013 drawn primarily from published data, compared with national Church of England statistics. Over 26% more clergy dyads were found than previously thought, with many active in ministry. A wide variety of ministry patterns were identified, including a higher than normal percentage in non-parochial roles, supporting previous research noting high levels of boundary enmeshment and absorptiveness. Considerable gender inequality prevailed in shared parochial settings in spite of women having been ordained priest for nearly 20 years, with very few wives holding more senior positions than their husbands, while female CMC are more likely to be dignitaries than other ordained women

Var2CSA Minimal CSA Binding Region Is Located within the N-Terminal Region

Var2CSA, a key molecule linked with pregnancy-associated malaria (PAM), causes sequestration of Plasmodium falciparum infected erythrocytes (PEs) in the placenta by adhesion to chondroitin sulfate A (CSA). Var2CSA possesses a 300 kDa extracellular region composed of six Duffy-binding like (DBL) domains and a cysteine-rich interdomain region (CIDRpam) module. Although initial studies implicated several individual var2CSA DBL domains as important for adhesion of PEs to CSA, new studies revealed that these individual domains lack both the affinity and specificity displayed by the full-length extracellular region. Indeed, recent evidence suggests the presence of a single CSA-binding site formed by a higher-order domain organization rather than several independent binding sites located on the different domains. Here, we search for the minimal binding region within var2CSA that maintains high affinity and specificity for CSA binding, a characteristic feature of the full-length extracellular region. Accordingly, truncated recombinant var2CSA proteins comprising different domain combinations were expressed and their binding characteristics assessed against different sulfated glycosaminoglycans (GAGs). Our results indicate that the smallest region within var2CSA with similar binding properties to those of the full-length var2CSA is DBL1X-3X. We also demonstrate that inhibitory antibodies raised in rabbit against the full-length DBL1X-6ε target principally DBL3X and, to a lesser extent, DBL5ε. Taken together, our results indicate that efforts should focus on the DBL1X-3X region for developing vaccine and therapeutic strategies aimed at combating PAM

Case studies of Roots, Tubers and Bananas seed systems.

The seed systems of RTB (root, tuber, and banana) crops are unique because they are propagated from vegetative parts of the plant, not from true seed. RTB seed is thus bulkier, more perishable, and more subject to the attacks of pests and diseases than is true seed. Because of this, there is often a gap between potential and real crop yields, which seed interventions seek to narrow. Seed systems are formal or informal networks of people and organizations that produce, plant, and distribute seed. Informal systems may deliver low quality seed, but not always. This book describes 13 RTB seed system interventions, using a framework based on the concepts of seed availability, access, and quality. The 13 case studies included (1) a potato-growers’ association in Ecuador, (2) a hydroponic seed potato in Peru, (3) a yam seed technology in Nigeria, (4) a banana and plantain project in Ghana, (5) a sweetpotato seed project in Tanzania and (6) one in Rwanda, (7) a seed potato system in Kenya, (8) cassava in Nicaragua, (9) seed potato in Malawi, (10) disease-resistant cassava varieties in seven African countries, (11) a tissue culture banana project, (12) an emergency plantain and banana project in East Africa, and (13) a large cassava seed project in six African countries

The Effect of Antioxidant Supplementation on Fatigue during Exercise: Potential Role for NAD+(H)

This study compared serum pyridine levels (NAD+ /NADH) in trained (n = 6) and untrained (n = 7) subjects after continuous progressive exercise at 50%, 70% then 95% of physical work capacity until fatigue (TTF) after consuming a placebo or antioxidant (AO) cocktail (Lactaway©). An increase of 17% in TTF was observed in AO as compared to placebo (p = 0.032). This was accompanied by a significant increase in serum NAD+ levels (p = 0.037) in the AO supplemented group post exercise. The increases in NAD+ and improved endurance reflect lower oxidative stress-induced suppression of aerobic respiration

The humoral response to Plasmodium falciparum VarO rosetting variant and its association with protection against malaria in Beninese children

<p>Abstract</p> <p>Background</p> <p>The capacity of <it>Plasmodium falciparum</it>-infected erythrocytes to bind uninfected erythrocytes (rosetting) is associated with severe malaria in African children. Rosetting is mediated by a subset of the variant surface antigens PfEMP1 targeted by protective antibody responses. Analysis of the response to rosette-forming parasites and their PfEMP1 adhesive domains is essential for understanding the acquisition of protection against severe malaria. To this end, the antibody response to a rosetting variant was analysed in children recruited with severe or uncomplicated malaria or asymptomatic <it>P. falciparum </it>infection.</p> <p>Methods</p> <p>Serum was collected from Beninese children with severe malaria, uncomplicated malaria or <it>P. falciparum </it>asymptomatic infection (N = 65, 37 and 52, respectively) and from immune adults (N = 30) living in the area. Infected erythrocyte surface-reactive IgG, rosette disrupting antibodies and IgG to the parasite crude extract were analysed using the single variant Palo Alto VarO-infected line. IgG, IgG1 and IgG3 to PfEMP1-varO-derived NTS-DBL1α₁, CIDRγ and DBL2βC2 recombinant domains were analysed by ELISA. Antibody responses were compared in the clinical groups. Stability of the response was studied using a blood sampling collected 14 months later from asymptomatic children.</p> <p>Results</p> <p>Seroprevalence of erythrocyte surface-reactive IgG was high in adults (100%) and asymptomatic children (92.3%) but low in children with severe or uncomplicated malaria (26.1% and 37.8%, respectively). The IgG, IgG1 and IgG3 antibody responses to the varO-derived PfEMP1 domains were significantly higher in asymptomatic children than in children with clinical malaria in a multivariate analysis correcting for age and parasite density at enrolment. They were essentially stable, although levels tended to decrease with time. VarO-surface reactivity correlated positively with IgG reactivity to the rosetting domain varO-NTS-DBL1α₁. None of the children sera, including those with surface-reactive antibodies possessed anti-VarO-rosetting activity, and few adults had rosette-disrupting antibodies.</p> <p>Conclusions</p> <p>Children with severe and uncomplicated malaria had similar responses. The higher prevalence and level of VarO-reactive antibodies in asymptomatic children compared to children with malaria is consistent with a protective role for anti-VarO antibodies against clinical falciparum malaria. The mechanism of such protection seems independent of rosette-disruption, suggesting that the cytophilic properties of antibodies come into play.</p

Crystal Structure of the Complex mAb 17.2 and the C-Terminal Region of Trypanosoma cruzi P2β Protein: Implications in Cross-Reactivity

Patients with Chronic Chagas' Heart Disease possess high levels of antibodies against the carboxyl-terminal end of the ribosomal P2ß protein of Trypanosoma cruzi (TcP2ß). These antibodies, as well as the murine monoclonal antibody (mAb) 17.2, recognize the last 13 amino acids of TcP2ß (called the R13 epitope: EEEDDDMGFGLFD) and are able to cross-react with, and stimulate, the ß1 adrenergic receptor (ß1-AR). Indeed, the mAb 17.2 was able to specifically detect human β1-AR, stably transfected into HEK cells, by flow cytometry and to induce repolarisation abnormalities and first degree atrioventricular conduction block after passive transfer to naïve mice. To study the structural basis of this cross-reactivity, we determined the crystal structure of the Fab region of the mAb 17.2 alone at 2.31 Å resolution and in complex with the R13 peptide at 1.89 Å resolution. We identified as key contact residues on R13 peptide Glu3, Asp6 and Phe9 as was previously shown by alanine scanning. Additionally, we generated a model of human β1-AR to elucidate the interaction with anti-R13 antibodies. These data provide an understanding of the molecular basis of cross-reactive antibodies induced by chronic infection with Trypanosoma cruzi