Integrated Circuitry to Detect Slippage Inspired by Human Skin and Artificial Retinas

This paper presents a bioinspired integrated tactile coprocessor that is able to generate a warning in the case of slippage via the data provided by a tactile sensor. Some implementations use different layers of piezoresistive and piezoelectric materials to build upon the raw sensor and obtain the static (pressure) as well as the dynamic (slippage) information. In this paper, a simple raw sensor is used, and a circuitry is implemented, which is able to extract the dynamic information from a single piezoresistive layer. The circuitry was inspired by structures found in human skin and retina, as they are biological systems made up of a dense network of receptors. It is largely based on an artificial retina , which is able to detect motion by using relatively simple spatial temporal dynamics. The circuitry was adapted to respond in the bandwidth of microvibrations produced by early slippage, resembling human skin. Experimental measurements from a chip implemented in a 0.35-mum four-metal two-poly standard CMOS process are presented to show both the performance of the building blocks included in each processing node and the operation of the whole system as a detector of early slippage.Ministerio de Economía y Competitividad TEC2006-12376-C02-01Gobierno de España TEC2006- 1572

Integrated circuit interface for artificial skins

Artificial sensitive skins are intended to emulate the human skin to improve the skills of robots and machinery in complex unstructured environments. They are basically smart arrays of pressure sensors. As in the case of artificial retinas, one problem to solve is the management of the huge amount of information that such arrays provide, especially if this information should be used by a central processing unit to implement some control algorithms. An approach to manage such information is to increment the signal processing performed close to the sensor in order to extract the useful information and reduce the errors caused by long wires. This paper proposes the use of voltage to frequency converters to implement a quite straightforward analog to digital conversion as front end interface to digital circuitry in a smart tactile sensor. The circuitry commonly implemented to read out the information from a piezoresistive tactile sensor can be modified to turn it into an array of voltage to frequency converters. This is carried out in this paper, where the feasibility of the idea is shown through simulations and its performance is discussed.Gobierno de España TEC2006-12376-C02-01, TEC2006-1572

Phantom Membrane Microfluidic Cross-Flow Filtration Device for the Direct Optical Detection of Water Pollutants

The diffusion of autonomous sensing platforms capable of a remote large-scale surveillance of environmental water basins is currently limited by the cost and complexity of standard analytical methods. In order to create a new generation of water analysis systems suitable for continuous monitoring of a large number of sites, novel technical solutions for fluid handling and detection are needed. Here we present a microfluidic device hosting a perfluorinated microporous membrane with refractive index similar to that of water, which enables the combination of filtration and label-free sensing of adsorbing substances, mainly pollutants, in environmental water samples. The cross-flow design of the microfluidic device avoids the clogging of the membrane due to particulate, whereas molecules with some hydrophobic moiety contained in the crossing flow are partially retained and their adhesion on the inner surface of the membrane yields an increase of light scattering intensity, which can be easily measured using a simple instrument based on Light Emitting Diode illumination. By cycling sample water and pure water as a reference, we demonstrate the detection of 0.5 microM of a model cationic surfactant and regeneration of the sensing surface. The optical response of the membrane sensor was characterized using a simple theoretical model that enables to quantify the concentration of target molecules from the amplitude and kinetics of the measured binding curves. The device was tested with real water samples containing large amount of environmental particles, without showing clogging of the membrane, and enabling nonspecific quantification of adsorbing substances in a few minutes.This project has received funding from the European Union’s Seventh Framework Programme (FP7) for Research, Technological Development and Demonstration through the NAPES project(grant agreement no. 604241). FBL acknowledges the Ramón y CajalProgramme (Ministerio de Economía y Competitividad), Spain. FBL personally acknowledges to Elkartek (KK-2015/00088) Grant from the Gobierno Vasco and funding support from Gobierno de España, Ministerio de Economia y Competitividad, with Grant No. BIO2016-80417-P and to Marian M. De Pancorbo for letting him to use her laboratory facilities at UPV/EHU. PSA was generously provided byAdhesive Research, Ireland. We thank Aurora Giavazzi for helping in the collection of river water samples

Tunable Superparamagnetic Ring (tSPRing) for Droplet Manipulation

The manipulation of droplets by magnetic field forms the basis of a fascinating technology that is currently in development. Often, the movement of droplets with magnets involves adding magnetic particles in or around the droplet; alternatively, magneto responsive surfaces may also be used. This work, presents and characterizes experimentally the formation and properties of a tunable superparamagnetic ring (tSPRing), which precisely adjusts itself around a water droplet, due to liquid-liquid interaction, and enables the physical manipulation of droplets. The ring is made of an oil-based ferrofluid, a stable suspension of ferromagnetic particles in an oily phase. It appears spontaneously due to the oil-water interfacial interaction under the influence of a magnetic field. The ferrofluid-water interaction resembles a cupcake assembly, with the surrounding ring only at the base of the droplet. The ring is analogous to a soft matter ring magnet, showing dipole repulsive forces, which stabilizes the droplets on a surface. It enables robust, controllable and programmable manipulation of enclosed water droplets. This work opens the door to new applications in open surface upside or upside-down microfluidics and lays the groundwork for new studies on tunable interfaces between two immiscible liquids.Authors acknowledge funding support from MaMi project, funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No. 766007, from the Basque Government, under Grupos Consolidados with Grant No. IT1271-19 and from Gobierno de España, Ministerio de Economia y Competitividad, with Grant No. BIO2016-80417-P (AEI/FEDER, UE

Tactile on-chip pre-processing with techniques from artificial retinas

The interest in tactile sensors is increasing as their use in complex unstructured environments is demanded, like in tele-presence, minimal invasive surgery, robotics etc. The matrix of pressure data these devices provide can be managed with many image processing algorithms to extract the required information. However, as in the case of vision chips or artificial retinas, problems arise when the array size and the computation complexity increase. Having a look to the skin, the information collected by every mechanoreceptor is not carried to the brain for its processing, but some complex pre-processing is performed to fit the limited throughput of the nervous system. This is specially important for high bandwidth demanding tasks. Experimental works report that neural response of skin mechanoreceptors encodes the change in local shape from an offset level rather than the absolute force or pressure distributions. This is also the behavior of the retina, which implements a spatio-temporal averaging. We propose the same strategy in tactile preprocessing, and we show preliminary results when it faces the detection of the slip, which involves fast real-time processing.Ministerio de Ciencia y Tecnología TIC2003 - 09817-C0

Reusable Ionogel-based Photo-actuators in a Lab-on-a-disc

This paper describes the design, fabrication and performance of a reusable ionogel-based photo-actuator, in-situ photopolymerised into a lab-on-a-disc microfluidic device, for flow control. The ionogel provides an effective barrier to liquids during storage of reagents and spinning of the disc. A simple LED (white light) triggers actuation of the ionogel for selective and precise channel opening at a desired location and time. The mechanism of actuation is reversible, and regeneration of the actuator is possible with an acid chloride solution. In order to achieve regeneration, the Lab-on-a-Disc device was designed with a microchannel connected perpendicularly to the bottom of the ionogel actuator (regeneration channel). This configuration allows the acid solution to reach the actuator, independently from the main channel, which initiates ionogel swelling and main channel closure, and thereby enables reusability of the whole device.Economía y Competitividad), Spain. This project has receivedfunding from the European Union Seventh Framework Programme(FP7) for Research, Technological Development and Demonstrationunder grant agreement no. 604241. JS and FBL acknowledge fund-ing support from Gobierno de Espa˜na, Ministerio de Economía yCompetitividad, with Grant No. BIO2016-80417-P and personallyacknowledge to Marian M. De Pancorbo for letting them to use herlaboratory facilities at UPV/EHU. A.T., L.F., and D.D. are grateful forfinancial support from the Marie Curie Innovative Training Net-work OrgBIO (Marie Curie ITN, GA607896) and Science FoundationIreland (SFI) under the Insight Centre for Data Analytics initiative,Grant Number SFI/12/RC/2289

Advances in Microtechnology for Improved Cytotoxicity Assessment

In vitro cytotoxicity testing is essential in the pharmaceutical and environmental industry to study the effects of potential harmful compounds for human health. Classical assays present several disadvantages: they are commonly based on live-death labelling, are highly time consuming and/or require skilled personnel to be performed. The current trend is to reduce the number of required cells and the time during the analysis, while increasing the screening capability and the accuracy and sensitivity of the assays, aiming single cell resolution. Microfabrication and surface engineering are enabling novel approaches for cytotoxicity assessment, offering high sensitivity and the possibility of automation in order to minimize user intervention. This review aims to overview the different microtechnology approaches available in this field, focusing on the novel developments for high-throughput, dynamic and real time screening of cytotoxic compounds.Funding support from: University of the Basque Country (PIF16/204), the funding support from Gobierno de España, Ministerio de Economía y Competitividad, with Grant No. BIO2016-80417-P (AEI/FEDER, UE) and Gobierno Vasco under grand IT1271-19

Low‑cost origami fabrication of 3D self‑aligned hybrid microfluidic structures

[EN] 3D microfluidic device fabrication methods are normally quite expensive and tedious. In this paper, we present an easy and cheap alternative wherein thin cyclic olefin polymer (COP) sheets and pressure sensitive adhesive(PSA) were used to fabricate hybrid 3D microfluidic structures, by the Origami technique, which enables the fabrication of microfluidic devices without the need of any alignment tool. The COP and PSA layers were both cut simultaneously using a portable, low-cost plotter allowing for rapid prototyping of a large variety of designs in a single production step. The devices were then manually assembled using the Origami technique by simply combining COP and PSA layers and mild pressure. This fast fabrication method was applied, as proof of concept, to the generation of a micromixer with a 3D-stepped serpentine design made of ten layers in less than 8 min. Moreover, the micromixer was characterized as a function of its pressure failure, achieving pressures of up to 1000 mbar. This fabrication method is readily accessible across a large range of potential end users, such as educational agencies (schools,universities), low-income/developing world research and industry or any laboratory without access to clean room facilities, enabling the fabrication of robust, reproducible microfluidic devices.Fernando Benito-Lopez acknowledges the Ramón y Cajal Programme (Ministerio de Economía y Competitividad), Spain. This project has received funding from the European Union´s Seventh Framework Programme (FP7) for Research, Technological Development and Demonstration under Grant agreement no. 604241. LBD personally acknowledges to Elkartek (KK-2015/00088) Grant form the Gobierno Vasco. JS and FBL personally acknowledge Marian Martínez de Pancorbo for let them use her laboratory facilities at UPV/EHU. Authors also acknowledge Adhesive Research for the donation of the PSA samples and to Iñaki Veci for the drawing of the 3D scheme

Imidazole-based ionogel as room temperature benzene and formaldehyde sensor

A room temperature benzene and formaldehyde gas sensor system with an ionogel as sensing material is presented. The sensing layer is fabricated employing poly(N-isopropylacrylamide) polymerized in the presence of 1-ethyl-3-methylimidazolium ethylsulfate ionic liquid onto gold interdigitated electrodes. When the ionogel is exposed to increasing formaldehyde concentrations employing N2as a carrier gas, a more stable response is observed in comparison to the bare ionic liquid, but no difference insensitivity occurs. On the other hand, when air is used as carrier gas the sensitivity of the system towards formaldehyde is decreased by one order of magnitude. At room temperature, the proposed sensor exhibited in air higher sensitivities to benzene, at concentrations ranging between 4 and 20 ppm resulting, in a limit of detection of 47 ppb, which is below the standard permitted concentrations. The selectivity of the IL towards HCHO and C6H6is demonstrated by the absence of response when another IL is employed. Humidity from the ambient air slightly affects the resistance of the system proving the protective role of the polymeric matrix. Furthermore, the gas sensor system showed fast response/recovery times considering the thickness of the material, suggesting that ionogel materials can be used as novel and highly efficient volatile organic compounds sensors operating at room temperature.This work was supported by the Ministry of Economy and Competitiveness (MINECO) of Spain under the TEMIN-++AIR programs. N.G.-G. was supported by a PhD fellowship from the University of Navarra. F. B.-L. acknowledges the funding support from Gobierno de España, Ministerio de Economía y Competitividad, with Grant No. BIO2016-80417-P, and Gobierno Vasco Dpto. Educación for the consolidation of the research groups (IT1271-19)

Predicting Dimensions in Microfluidic Paper Based Analytical Devices

The main problem for the expansion of the use of microfluidic paper-based analytical devices and, thus, their mass production is their inherent lack of fluid flow control due to its uncontrolled fabrication protocols. To address this issue, the first step is the generation of uniform and reliable microfluidic channels. The most common paper microfluidic fabrication method is wax printing, which consists of two parts, printing and heating, where heating is a critical step for the fabrication of reproducible device dimensions. In order to bring paper-based devices to success, it is essential to optimize the fabrication process in order to always get a reproducible device. Therefore, the optimization of the heating process and the analysis of the parameters that could affect the final dimensions of the device, such as its shape, the width of the wax barrier and the internal area of the device, were performed. Moreover, we present a method to predict reproducible devices with controlled working areas in a simple manner.The authors would like to acknowledge funding support from Gobierno de España, Ministerio de Economía y Competitividad, with Grant No. BIO2016-80417-P (AEI/FEDER, UE), the Gobierno Vasco Dpto. Educación for the consolidation of the research groups (IT1271-19) and from Proyectos Colaborativos from the University of the Basque Country UPV/EHU, BIOPLASMOF (COLAB19/05). This project received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 778001