Hunting for differentially expressed genes

Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field

TRANSLATING THE PROBLEMS OF THE ELDERLY INTO EFFECTIVE POLICIES: AN ANALYSIS OF FILIAL ATTITUDES

This paper examines the political implications of current decision making efforts that underpin recent cost-cutting measures of federally supported programs serving the elderly. Current policy changes that favor the private sector (e.g., shifts of the cost of care to other levels of government and to elderly individuals and their families) have also generated a renewed interest in the policy of mandating family responsibility. The paper also examines attitudes concerning filial responsibility, such as multigenerational living. The inadequacy of current policy analysis that has encouraged the disestablishment of the current long-term care system and the shifting of care costs to the private sector, especially to the individual and the family are also examined. Copyright 1989 by The Policy Studies Organization.