Direction of Translation and Size of Bacteriophage [var phi]X174 Cistrons

Translation of the bacteriophage [var phi]X174 genome follows cistron order D-E-F-G-H-A-B-C. To establish this, the position of a nonsense mutation on the genetic map was compared with the physical size (molecular weight) of the appropriate protein fragment generated in nonpermissive cells. Distances on the [var phi]X174 genetic map and distances on a physical map constructed from the molecular weights of [var phi]X174 proteins and protein fragments are proportional over most of the genome with the exception of the high recombination region within cistron A

Genetic Map of Bacteriophage [var phi]X174

Bacteriophage [var phi]X174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses. The genetic map is circular with a total length of 24 × 10−4wt recombinants per progeny phage. The cistron order is D-E-F-G-H-A-B-C. High negative interference is seen, consistent with a small closed circular deoxyribonucleic acid molecule as a genome

Genetic Recombination in Bacteriophage {varphi}X174

Genetic recombination in bacteriophage {varphi}X174 usually takes place early in the infection process and involves two parental replicative form (double-stranded) DNA molecules. The host recA protein is required; none of the nine known {varphi}X174 cistron products is essential. The products of a single recombination event are nonreciprocal and asymmetric. Typically, only one of the parental genotypes and one recombinant genotype are recovered from a single cell. An alternative, less efficient recombination mechanism which requires an active {varphi}X174 cistron A protein is observed in the absence of the host recA gene product

Advanced crew procedures development techniques

The development of an operational computer program, the Procedures and Performance Program (PPP), is reported which provides a procedures recording and crew/vehicle performance monitoring capability. The PPP provides real time CRT displays and postrun hardcopy of procedures, difference procedures, performance, performance evaluation, and training script/training status data. During post-run, the program is designed to support evaluation through the reconstruction of displays to any point in time. A permanent record of the simulation exercise can be obtained via hardcopy output of the display data, and via magnetic tape transfer to the Generalized Documentation Processor (GDP). Reference procedures data may be transferred from the GDP to the PPP

Life Cycle of a Torrenticolous Hawaiian Chironomid (Telmatogeton Torrenticola): Stream Flow and Microhabitat Effects

In this study we documented the instar densities and life cycle of Telmatogeton torrenticola Terry (Chironomidae : Telmatogetoninae) from Kinihapai Stream, Maui, Hawaii. Greatest larval densities of this midge are found on substrates of high velocity, shallow flows of cascades, and splash zones of waterfalls, with lower densities in riffles. In the summer of 1994 we compared the effects of two microhabitats (termed optimal and suboptimal) on inter-instar density and relative abundance. In a second year (1995), we evaluated the effect of long-term reduced stream flow on these variables only in optimal microhabitats. A significant reduction in stream flow from 1994 to 1995 was correlated with a similar reduction in larval densities that precluded larval colonization of suboptimal habitats in 1995, thereby preventing sampling in this microhabitat during that summer. Depth of optimal habitats of 1995 were significantly shallower than both habitats of 1994, with suboptimal habitats of 1994 the deepest. Total larval density was significantly higher in 1994 optimal habitats, while 1994 suboptimal and 1995 optimal habitats were statistically similar. Individual instar densities showed variable differences among habitats and years, with the first three instars always highest in 1994 optimal habitats. All instars and pupae were collected on most sampling dates in both years ; however, pupae were only collected on a two dates in suboptimal habitats of 1994. Based on larval size frequency histograms, T. torrenticola has a multivoltine, asynchronous life cycle, with continuous reproduction, which is variable among microhabitats characterized by different flow velocity and depth, and between years of differing stream discharge. Reduced stream flow during the summer of 1995 had effects of reducing densities and changing life cycle features similar to those found in 1994 (a year of higher stream flow) suboptimal habitats

A Spectacular VHE Gamma-Ray Outburst from PKS 2155-304 in 2006

Since 2002 the VHE (>100 GeV) gamma-ray flux of the high-frequency peaked BL Lac PKS 2155-304 has been monitored with the High Energy Stereoscopic System (HESS). An extreme gamma-ray outburst was detected in the early hours of July 28, 2006 (MJD 53944). The average flux above 200 GeV observed during this outburst is ~7 times the flux observed from the Crab Nebula above the same threshold. Peak fluxes are measured with one-minute time scale resolution at more than twice this average value. Variability is seen up to ~600 s in the Fourier power spectrum, and well-resolved bursts varying on time scales of ~200 seconds are observed. There are no strong indications for spectral variability within the data. Assuming the emission region has a size comparable to the Schwarzschild radius of a ~10^9 solar mass black hole, Doppler factors greater than 100 are required to accommodate the observed variability time scales.Comment: 4 pages, 3 figures; To appear in the Proceedings of the 30th ICRC (Merida, Mexico

Crew procedures development techniques

The study developed requirements, designed, developed, checked out and demonstrated the Procedures Generation Program (PGP). The PGP is a digital computer program which provides a computerized means of developing flight crew procedures based on crew action in the shuttle procedures simulator. In addition, it provides a real time display of procedures, difference procedures, performance data and performance evaluation data. Reconstruction of displays is possible post-run. Data may be copied, stored on magnetic tape and transferred to the document processor for editing and documentation distribution

Comparison of truck fuel consumption measurements with results of existing models and implications for road pavement LCA

Life Cycle Assessment (LCA) is increasingly used to evaluate the impact of all lifecycle phases of road pavements on the environment. From the late ‘90s, this technique has continuously evolved and improved, however, there are still limitations and uncertainties in the framework. In this regard, Santero et al (2011) showed that gaps still exist in the road pavement LCA methodology. More recently, Trupia et al (2016) highlighted how existing models of the impact of the road pavement condition on vehicle rolling resistance and hence, fuel consumption, can lead to very different results. This study presents a comparison between real measurements of truck fuel consumption from fleet manager’s databases, and results of existing pavement models, MIRAVEC, a model recently developed within an ERA-NET ROAD action, funded by the 6th framework programme of the EU, and HDM-4, one of the most widely used models for estimating vehicle operating costs in road asset management. The paper shows how far results of the considered models can be from reality and opens a discussion of the implications of these differences on pavement LCA and strategic decisions of managers of the road infrastructure